RMAR_YEAST
ID RMAR_YEAST Reviewed; 398 AA.
AC P02381; A0A0A7NYM3; A2KBL8; Q95946; Q9ZZW5;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 3.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ribosomal protein VAR1, mitochondrial;
DE AltName: Full=Mitochondrial small ribosomal subunit protein uS3m {ECO:0000303|PubMed:28154081};
GN Name=VAR1; OrderedLocusNames=Q0140;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6754087; DOI=10.1016/0092-8674(82)90258-6;
RA Hudspeth M.E.S., Ainley W.M., Shumard D.S., Butow R.A., Grossman L.I.;
RT "Location and structure of the var1 gene on yeast mitochondrial DNA:
RT nucleotide sequence of the 40.0 allele.";
RL Cell 30:617-626(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3549452; DOI=10.1016/0378-1119(86)90060-0;
RA de Zamaroczy M., Bernardi G.;
RT "The primary structure of the mitochondrial genome of Saccharomyces
RT cerevisiae -- a review.";
RL Gene 47:155-177(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9755179; DOI=10.1093/emboj/17.19.5796;
RA Sanchirico M.E., Fox T.D., Mason T.L.;
RT "Accumulation of mitochondrially synthesized Saccharomyces cerevisiae Cox2p
RT and Cox3p depends on targeting information in untranslated portions of
RT their mRNAs.";
RL EMBO J. 17:5796-5804(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9872396; DOI=10.1016/s0014-5793(98)01467-7;
RA Foury F., Roganti T., Lecrenier N., Purnelle B.;
RT "The complete sequence of the mitochondrial genome of Saccharomyces
RT cerevisiae.";
RL FEBS Lett. 440:325-331(1998).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION.
RX PubMed=7770043; DOI=10.1007/bf00293206;
RA Davis S.C., Ellis S.R.;
RT "Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into
RT ribosomal subunits requires the mitochondrially encoded Var1 protein.";
RL Mol. Gen. Genet. 247:379-386(1995).
RN [7]
RP IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11278769; DOI=10.1074/jbc.m010864200;
RA Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A.,
RA Jacquier A.;
RT "Identification of 12 new yeast mitochondrial ribosomal proteins including
RT 6 that have no prokaryotic homologues.";
RL J. Biol. Chem. 276:15861-15867(2001).
RN [8]
RP IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT spectrometric identification of its new components.";
RL Eur. J. Biochem. 269:5203-5214(2002).
RN [9]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=25609543; DOI=10.1038/ncomms7019;
RA Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT "Organization of the mitochondrial translation machinery studied in situ by
RT cryoelectron tomography.";
RL Nat. Commun. 6:6019-6019(2015).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.30 ANGSTROMS), AND SUBUNIT.
RX PubMed=28154081; DOI=10.1126/science.aal2415;
RA Desai N., Brown A., Amunts A., Ramakrishnan V.;
RT "The structure of the yeast mitochondrial ribosome.";
RL Science 355:528-531(2017).
CC -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC mitochondrial genome-encoded proteins, including at least some of the
CC essential transmembrane subunits of the mitochondrial respiratory
CC chain. The mitoribosomes are attached to the mitochondrial inner
CC membrane and translation products are cotranslationally integrated into
CC the membrane (PubMed:25609543, PubMed:28154081). uS3m is essential for
CC mitochondrial protein synthesis and required for the maturation of
CC small ribosomal subunits (PubMed:7770043). {ECO:0000269|PubMed:7770043,
CC ECO:0000305|PubMed:25609543, ECO:0000305|PubMed:28154081}.
CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-
CC SSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC and a large (54S) subunit. The 37S small subunit contains a 15S
CC ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC uS3m, uS4m and uS5m form the narrow entry site of the mRNA channel.
CC {ECO:0000269|PubMed:11278769, ECO:0000269|PubMed:12392552,
CC ECO:0000269|PubMed:28154081}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14576278}.
CC Note=Mitoribosomes are tethered to the mitochondrial inner membrane and
CC spatially aligned with the membrane insertion machinery through two
CC distinct membrane contact sites, formed by the 21S rRNA expansion
CC segment 96-ES1 and the inner membrane protein MBA1.
CC {ECO:0000269|PubMed:25609543}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS3 family.
CC {ECO:0000305}.
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DR EMBL; V00705; CAA24077.1; -; Genomic_DNA.
DR EMBL; L36900; AAA67536.1; -; Genomic_DNA.
DR EMBL; AJ010480; CAA09218.1; -; Genomic_DNA.
DR EMBL; KP263414; AIZ98895.1; -; Genomic_DNA.
DR PIR; S78670; R3BYM1.
DR RefSeq; NP_009320.1; NC_001224.1.
DR PDB; 5MRC; EM; 3.25 A; CC=1-398.
DR PDB; 5MRE; EM; 3.75 A; CC=1-398.
DR PDB; 5MRF; EM; 4.97 A; CC=1-398.
DR PDBsum; 5MRC; -.
DR PDBsum; 5MRE; -.
DR PDBsum; 5MRF; -.
DR AlphaFoldDB; P02381; -.
DR SMR; P02381; -.
DR BioGRID; 34782; 33.
DR ComplexPortal; CPX-1603; 37S mitochondrial small ribosomal subunit.
DR IntAct; P02381; 2.
DR STRING; 4932.Q0140; -.
DR MaxQB; P02381; -.
DR PaxDb; P02381; -.
DR PRIDE; P02381; -.
DR EnsemblFungi; Q0140_mRNA; Q0140; Q0140.
DR GeneID; 854586; -.
DR KEGG; sce:Q0140; -.
DR SGD; S000007275; VAR1.
DR VEuPathDB; FungiDB:Q0140; -.
DR eggNOG; ENOG502SVA8; Eukaryota.
DR HOGENOM; CLU_714008_0_0_1; -.
DR InParanoid; P02381; -.
DR OMA; YMNINND; -.
DR BioCyc; YEAST:G3O-34384-MON; -.
DR PRO; PR:P02381; -.
DR Proteomes; UP000002311; Mitochondrion.
DR RNAct; P02381; protein.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005761; C:mitochondrial ribosome; IDA:SGD.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD.
DR InterPro; IPR007980; Ribosomal_VAR1.
DR Pfam; PF05316; VAR1; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein.
FT CHAIN 1..398
FT /note="Ribosomal protein VAR1, mitochondrial"
FT /id="PRO_0000220076"
FT CONFLICT 169..170
FT /note="Missing (in Ref. 1; CAA24077 and 3; CAA09218)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="K -> N (in Ref. 1; CAA24077, 2; AAA67536 and 3;
FT CAA09218)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="N -> NNNNNNN (in Ref. 2; AAA67536)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 398 AA; 47123 MW; ECA1161E08E4205F CRC64;
MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN
NMNPAGANPV VHRIGPAGNI NNKLQHLNNM NNWNTQIYNY NKNMEIMNTM NDKLINKLLY
KMMTLKLNNM NINKIIMSKT INQHSLNKLN IKFYYYNNDI NNNNNNNNNN YYMNMMNKLM
NIMNNNMNNN LCNILSYYYK KKVTIEPIKL SYIYLNSDIF SKYISLNDMD KYNNGILTNY
QRMLNNIMPK LNDHNISMNY INNINNINNN KYNNMINLLN NNNNINNNNN YNNNNNNYIG
NINNIYNNMT IDNIPMDILM YKYLVGWSIK FKGRLSNNNG RTSTTNLLNG TFNNKKYLWS
NINNNYKLNY IPSNHNLYNN SNINKNGKYN IKVKLNFI
