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RME6_CAEEL
ID   RME6_CAEEL              Reviewed;        1093 AA.
AC   Q9GYH7; Q4VQC9; Q8MNR6;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Receptor-mediated endocytosis protein 6;
GN   Name=rme-6; ORFNames=F49E7.1;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP   INTERACTION WITH RAB5 AND ALPHA-ADAPTIN, AND MUTAGENESIS OF VAL-65;
RP   CYS-1017 AND TRP-1079.
RX   PubMed=15895077; DOI=10.1038/ncb1261;
RA   Sato M., Sato K., Fonarev P., Huang C.-J., Liou W., Grant B.D.;
RT   "Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the
RT   clathrin-coated pit.";
RL   Nat. Cell Biol. 7:559-569(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Acts both as a GTPase-activating protein (GAP) and a guanine
CC       nucleotide exchange factor (GEF), and participates in endocytosis. Acts
CC       by regulating the activation of rab-5 by exchanging bound GDP for free
CC       GTP at clathrin coated pits. {ECO:0000269|PubMed:15895077}.
CC   -!- SUBUNIT: Interacts with GDP-bound rab-5. Interacts with alpha-adaptin.
CC       {ECO:0000269|PubMed:15895077}.
CC   -!- INTERACTION:
CC       Q9GYH7; Q22601: apa-2; NbExp=3; IntAct=EBI-320814, EBI-5323248;
CC       Q9GYH7; P91857: rab-5; NbExp=2; IntAct=EBI-320814, EBI-2655330;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:15895077};
CC       Peripheral membrane protein {ECO:0000269|PubMed:15895077}. Cytoplasmic
CC       vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:15895077}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=Q9GYH7-1; Sequence=Displayed;
CC       Name=b;
CC         IsoId=Q9GYH7-2; Sequence=VSP_032369;
CC   -!- SIMILARITY: Belongs to the GAPVD1 family. {ECO:0000305}.
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DR   EMBL; AY750054; AAW73253.1; -; mRNA.
DR   EMBL; FO080361; CCD63167.1; -; Genomic_DNA.
DR   EMBL; FO080361; CCD63168.1; -; Genomic_DNA.
DR   PIR; T25788; T25788.
DR   PIR; T31057; T31057.
DR   RefSeq; NP_001294826.1; NM_001307897.1.
DR   RefSeq; NP_508208.2; NM_075807.3. [Q9GYH7-1]
DR   AlphaFoldDB; Q9GYH7; -.
DR   SMR; Q9GYH7; -.
DR   BioGRID; 45416; 3.
DR   DIP; DIP-25631N; -.
DR   IntAct; Q9GYH7; 2.
DR   STRING; 6239.F49E7.1a; -.
DR   EPD; Q9GYH7; -.
DR   PaxDb; Q9GYH7; -.
DR   PeptideAtlas; Q9GYH7; -.
DR   PRIDE; Q9GYH7; -.
DR   EnsemblMetazoa; F49E7.1a.1; F49E7.1a.1; WBGene00004377. [Q9GYH7-1]
DR   EnsemblMetazoa; F49E7.1b.1; F49E7.1b.1; WBGene00004377. [Q9GYH7-2]
DR   EnsemblMetazoa; F49E7.1b.2; F49E7.1b.2; WBGene00004377. [Q9GYH7-2]
DR   EnsemblMetazoa; F49E7.1b.3; F49E7.1b.3; WBGene00004377. [Q9GYH7-2]
DR   GeneID; 180463; -.
DR   KEGG; cel:CELE_F49E7.1; -.
DR   UCSC; F49E7.1a; c. elegans. [Q9GYH7-1]
DR   CTD; 180463; -.
DR   WormBase; F49E7.1a; CE38712; WBGene00004377; rme-6. [Q9GYH7-1]
DR   WormBase; F49E7.1b; CE26468; WBGene00004377; rme-6. [Q9GYH7-2]
DR   eggNOG; KOG2319; Eukaryota.
DR   GeneTree; ENSGT00940000156611; -.
DR   HOGENOM; CLU_012490_0_0_1; -.
DR   InParanoid; Q9GYH7; -.
DR   OMA; SCFLGKK; -.
DR   OrthoDB; 944088at2759; -.
DR   PhylomeDB; Q9GYH7; -.
DR   Reactome; R-CEL-8856828; Clathrin-mediated endocytosis.
DR   PRO; PR:Q9GYH7; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00004377; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR   GO; GO:0045334; C:clathrin-coated endocytic vesicle; IDA:WormBase.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005769; C:early endosome; IDA:WormBase.
DR   GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:WormBase.
DR   GO; GO:0032794; F:GTPase activating protein binding; ISS:UniProtKB.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR   GO; GO:0031267; F:small GTPase binding; IPI:WormBase.
DR   GO; GO:0006897; P:endocytosis; IMP:WormBase.
DR   GO; GO:0018996; P:molting cycle, collagen and cuticulin-based cuticle; IGI:WormBase.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IGI:WormBase.
DR   GO; GO:2000369; P:regulation of clathrin-dependent endocytosis; IMP:WormBase.
DR   GO; GO:0051223; P:regulation of protein transport; ISS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.10.506.10; -; 1.
DR   Gene3D; 1.20.1050.80; -; 1.
DR   InterPro; IPR001936; RasGAP_dom.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR003123; VPS9.
DR   InterPro; IPR045046; Vps9-like.
DR   InterPro; IPR037191; VPS9_dom_sf.
DR   PANTHER; PTHR23101; PTHR23101; 1.
DR   Pfam; PF00616; RasGAP; 1.
DR   Pfam; PF02204; VPS9; 1.
DR   SMART; SM00167; VPS9; 1.
DR   SUPFAM; SSF109993; SSF109993; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
DR   PROSITE; PS51205; VPS9; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasmic vesicle; Endocytosis; GTPase activation;
KW   Guanine-nucleotide releasing factor; Membrane; Reference proteome.
FT   CHAIN           1..1093
FT                   /note="Receptor-mediated endocytosis protein 6"
FT                   /id="PRO_0000324775"
FT   DOMAIN          140..355
FT                   /note="Ras-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT   DOMAIN          955..1093
FT                   /note="VPS9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00550"
FT   REGION          547..610
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          643..669
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        565..601
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        651..666
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..999
FT                   /note="Missing (in isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_032369"
FT   MUTAGEN         65
FT                   /note="V->D: In ar483; shows endocytic defects."
FT                   /evidence="ECO:0000269|PubMed:15895077"
FT   MUTAGEN         1017
FT                   /note="C->Y: In ar507; shows endocytic defects."
FT                   /evidence="ECO:0000269|PubMed:15895077"
FT   MUTAGEN         1079
FT                   /note="W->R: In b1015; shows endocytic defects."
FT                   /evidence="ECO:0000269|PubMed:15895077"
SQ   SEQUENCE   1093 AA;  124005 MW;  8C50161C988EF4B2 CRC64;
     MADIIERIRS QDEYREFYEL ATKIRNQKLL TDAELHSLNR HQQEVSDNEI KLLTEAWRSS
     YWNNVGRQLK NQALEPTIAC RLLKEISETE ALPAYKHVGH HFSTLDQLLT ILYNEPQSVA
     ELLNSIDRND ITSNDSTIQV LFHLVYSCAL YPEDELKIAQ VVCNLLKLQL YRSGSEMRTI
     LRKETSISTR FYKHFVELIH PTMVYLTKAL RKGVLDVIQL GSFWLDIDAK QSTARFLRDN
     KQNEDRLPEY RALVVSKLVD FVDTFLENIS KALPMLPPNL NWIIRDIFCS LYEVVDDVSA
     IELSHACKDM IVSNLICPAI ISPQKFGVVD NDVRVGTIVN HNLVQIAMII QMISLREFES
     PPEEYKEFLS QCRNTHLISE MMDALLVEKL APDVEITSLI ANGTAESDMA TKSSFVGTVA
     DVNVLIKIIR NAPSTSDAKL SRILSVCDKL PATISSTTQK SHFDNLEASP KISTLRNIHR
     KVQHSFKRTG DSFNDINELG AKQGEALAFG KENFDLFYLE YSPKNYKDVA EADRLKKKAE
     ERRQLADLEK LLEPTPAPKE TPVENNLIDF SSGSAETNSE HLSDSTSVSP EPLTSTEELQ
     IGDQGVGGEQ RGKLAKLKSL SDRMKKGITQ SNTLSDIREH LRRSGSFVKP PPSGIPTSSS
     EQNLPDVATG PRDDILAKYA SISSIKEQKP ALGNLIDGTA SASSTPRKEP LEPYYSSENL
     VGCRAFQDTL RKMITVLGNI SYLPRIGYRN ETKQNEWDKK VLLGRFLDGV LVETEHRREY
     GLAAQLREVK RCIELFEHAG VEILMDHLKL NEAEQDKIVN GMREERASLM RKSNDISSLE
     QRVLLNRRLT EQILVDFLMR TFLETGFNNK HTVGKTQEVT AVLKFYDEFK YLRAQDERAE
     FLKNLLTFLR DRLMQNVDWN FATDTMMSRA MTTIERYVIF AVYDNAFYPN RDADHHRDKL
     LRGTIAKVSD VVTPVNDFLK IPEHLHGEAP WPSAQAELSM LDIYVTAQDK LNCVVRCCDV
     INNLVALSSK NAVASADDLT PVLVFVIIKA NPRALLSNVQ FVETFAGDRI ESGRDAYYWV
     NFKSAVEYIK TIL
 
 
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