ID   RMI1_ARATH              Reviewed;         644 AA.
AC   Q5XUX6; Q9FMU8;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=RecQ-mediated genome instability protein 1;
DE   AltName: Full=BLM-associated protein of 75 kDa homolog;
DE            Short=BLAP75;
GN   Name=RMI1; OrderedLocusNames=At5g63540; ORFNames=MLE2.17;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA   Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT   features of the regions of 1,191,918 bp covered by seventeen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:401-414(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, INTERACTION WITH TOP3A, AND DISRUPTION PHENOTYPE.
RX   PubMed=19096507; DOI=10.1371/journal.pgen.1000285;
RA   Hartung F., Suer S., Knoll A., Wurz-Wildersinn R., Puchta H.;
RT   "Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are
RT   essential for resolution of meiotic recombination intermediates in
RT   Arabidopsis thaliana.";
RL   PLoS Genet. 4:E1000285-E1000285(2008).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19096505; DOI=10.1371/journal.pgen.1000309;
RA   Chelysheva L., Vezon D., Belcram K., Gendrot G., Grelon M.;
RT   "The Arabidopsis BLAP75/Rmi1 homologue plays crucial roles in meiotic
RT   double-strand break repair.";
RL   PLoS Genet. 4:E1000309-E1000309(2008).
RN   [6]
RP   FUNCTION.
RX   PubMed=23956219; DOI=10.1093/nar/gkt730;
RA   Bonnet S., Knoll A., Hartung F., Puchta H.;
RT   "Different functions for the domains of the Arabidopsis thaliana RMI1
RT   protein in DNA cross-link repair, somatic and meiotic recombination.";
RL   Nucleic Acids Res. 41:9349-9360(2013).
CC   -!- FUNCTION: Essential component of the RMI complex, a complex that plays
CC       an important role in the resolution step of homologous recombination,
CC       in a process called Holliday Junction dissolution, to limit DNA
CC       crossover formation in cells. Together with TOP3A, is essential for the
CC       resolution of meiotic recombination intermediates, a step that prevents
CC       entanglement of the parental chromosomes. {ECO:0000269|PubMed:19096505,
CC       ECO:0000269|PubMed:19096507, ECO:0000269|PubMed:23956219}.
CC   -!- SUBUNIT: Component of the RMI complex, containing at least TOP3A and
CC       RMI1. The RMI complex interacts with RECQL4A (By similarity).
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q5XUX6-1; Sequence=Displayed;
CC   -!- DISRUPTION PHENOTYPE: sterile due to extensive chromosome breakage in
CC       meiosis I. {ECO:0000269|PubMed:19096505, ECO:0000269|PubMed:19096507}.
CC   -!- SIMILARITY: Belongs to the RMI1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08819.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB007649; BAB08819.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED97765.1; -; Genomic_DNA.
DR   EMBL; AY735746; AAU44616.1; -; mRNA.
DR   EMBL; AY954880; AAX55206.1; -; mRNA.
DR   RefSeq; NP_201159.2; NM_125749.5. [Q5XUX6-1]
DR   AlphaFoldDB; Q5XUX6; -.
DR   SMR; Q5XUX6; -.
DR   STRING; 3702.AT5G63540.2; -.
DR   PRIDE; Q5XUX6; -.
DR   ProteomicsDB; 228150; -. [Q5XUX6-1]
DR   EnsemblPlants; AT5G63540.1; AT5G63540.1; AT5G63540. [Q5XUX6-1]
DR   GeneID; 836473; -.
DR   Gramene; AT5G63540.1; AT5G63540.1; AT5G63540. [Q5XUX6-1]
DR   KEGG; ath:AT5G63540; -.
DR   Araport; AT5G63540; -.
DR   eggNOG; ENOG502QPJ3; Eukaryota.
DR   HOGENOM; CLU_014468_1_0_1; -.
DR   OMA; MQFALMN; -.
DR   PhylomeDB; Q5XUX6; -.
DR   PRO; PR:Q5XUX6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q5XUX6; baseline and differential.
DR   Genevisible; Q5XUX6; AT.
DR   GO; GO:0016604; C:nuclear body; IBA:GO_Central.
DR   GO; GO:0031422; C:RecQ family helicase-topoisomerase III complex; IBA:GO_Central.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:UniProtKB.
DR   Gene3D; 2.40.50.770; -; 1.
DR   InterPro; IPR033472; DUF1767.
DR   InterPro; IPR039215; RMI1.
DR   InterPro; IPR032199; RMI1_C.
DR   InterPro; IPR013894; RMI1_N.
DR   InterPro; IPR042470; RMI1_N_C_sf.
DR   PANTHER; PTHR14790; PTHR14790; 1.
DR   Pfam; PF16099; RMI1_C; 1.
DR   Pfam; PF08585; RMI1_N; 1.
DR   SMART; SM01161; DUF1767; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Meiosis; Reference proteome.
FT   CHAIN           1..644
FT                   /note="RecQ-mediated genome instability protein 1"
FT                   /id="PRO_0000429782"
FT   REGION          1..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..54
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   644 AA;  69970 MW;  8E51CC40D8007C11 CRC64;
     MRRRRLQLRY SSDEEEEDET GTSGVGDSVQ SFGIASSIQP ETSVSSNSNP NPGERIAISE
     VEIIDVFGNP QPTPPDSSIP TPYPVYPSES VSGNDYESPI SEVLSRMGIK LKREWWVSCL
     SGLETSIPQF SYLDVAAKAK HCFEQFMFFD MNLCGGGVLP PNVASMNRIE LAGPFVLQVD
     EIVNIGCPLK GRYENANAGL KRCLKLSMTD GVQRVFGMEY RPIKDLQVLA PAGLKIVVSS
     VQVRHGLLML VPEIVEVLGG MVEELEEARK RLVVEVNKPP RGKRTRVGVV PSLTTRATLA
     AWSLNGNDTG NHVHDTGNHV NSSASGNASH TQANQGIPVH VTRTHNSSRA MDEPLASTNV
     GATVSRVEHM QIDTASAHGE RTFSDIHSTS SNIHRAASTA GTGTSCSGAC SGARSFANNV
     GGNSLDQTSN VTSFVEEMHI DTGRVRDTTT HIYGSDSGGV AAEFSNMVVD LEGPSVLSTN
     TEKPFTYLAE LSQKWAVMKD TIHFVQGRIK CFLTGVKKFQ FKQQSTYELL CYVDDGSLIC
     EILLHNDVVQ KRIGHSSMEV TAALSSSAPT SLNAMMKEKL KRFQLFLADF EGIMVVEMNR
     SSQYPVAIEM NQGCSLTDAR LLLDRIKSSS RTSSSLNPVV VLSP