RMI1_YEAST
ID RMI1_YEAST Reviewed; 241 AA.
AC Q02685; D6W3Y8;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=RecQ-mediated genome instability protein 1;
GN Name=RMI1 {ECO:0000312|SGD:S000005945};
GN Synonyms=NCE4 {ECO:0000312|SGD:S000005945}; OrderedLocusNames=YPL024W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1] {ECO:0000312|EMBL:AAB68163.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3] {ECO:0000305}
RP FUNCTION IN MMS RESISTANCE.
RX PubMed=12482937; DOI=10.1073/pnas.262669299;
RA Chang M., Bellaoui M., Boone C., Brown G.W.;
RT "A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals
RT genes required for S phase progression in the presence of DNA damage.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16934-16939(2002).
RN [4] {ECO:0000305}
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5] {ECO:0000305}
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH SGS1 AND TOP3.
RX PubMed=15889139; DOI=10.1038/sj.emboj.7600684;
RA Chang M., Bellaoui M., Zhang C., Desai R., Morozov P., Delgado-Cruzata L.,
RA Rothstein R., Freyer G.A., Boone C., Brown G.W.;
RT "RMI1/NCE4, a suppressor of genome instability, encodes a member of the
RT RecQ helicase/Topo III complex.";
RL EMBO J. 24:2024-2033(2005).
RN [7] {ECO:0000305}
RP FUNCTION, DNA-BINDING, AND INTERACTION WITH SGS1 AND TOP3.
RX PubMed=15899853; DOI=10.1128/mcb.25.11.4476-4487.2005;
RA Mullen J.R., Nallaseth F.S., Lan Y.Q., Slagle C.E., Brill S.J.;
RT "Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3
RT complex.";
RL Mol. Cell. Biol. 25:4476-4487(2005).
CC -!- FUNCTION: Structure-specific DNA-binding protein with a preference for
CC cruciform structures. Also binds single-stranded DNA (ssDNA). Functions
CC together with SGS1 and TOP3 to maintain genome integrity. Essential for
CC proper meiotic cell division. Required for normal S-phase progression
CC and DNA damage response. Required for resistance to the DNA-damaging
CC agent methyl methanesulfonate (MMS). {ECO:0000269|PubMed:12482937,
CC ECO:0000269|PubMed:15889139, ECO:0000269|PubMed:15899853}.
CC -!- SUBUNIT: Forms a complex with SGS1 and TOP3.
CC {ECO:0000269|PubMed:15889139, ECO:0000269|PubMed:15899853}.
CC -!- INTERACTION:
CC Q02685; P35187: SGS1; NbExp=7; IntAct=EBI-38690, EBI-17059;
CC Q02685; P13099: TOP3; NbExp=9; IntAct=EBI-38690, EBI-19365;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1500 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RMI1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U36624; AAB68163.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11404.1; -; Genomic_DNA.
DR PIR; S63458; S63458.
DR RefSeq; NP_015301.1; NM_001183838.1.
DR AlphaFoldDB; Q02685; -.
DR BioGRID; 36153; 301.
DR ComplexPortal; CPX-1071; RecQ helicase-Topo III complex.
DR DIP; DIP-4039N; -.
DR IntAct; Q02685; 3.
DR MINT; Q02685; -.
DR STRING; 4932.YPL024W; -.
DR iPTMnet; Q02685; -.
DR PaxDb; Q02685; -.
DR PRIDE; Q02685; -.
DR EnsemblFungi; YPL024W_mRNA; YPL024W; YPL024W.
DR GeneID; 856083; -.
DR KEGG; sce:YPL024W; -.
DR SGD; S000005945; RMI1.
DR VEuPathDB; FungiDB:YPL024W; -.
DR eggNOG; KOG3683; Eukaryota.
DR HOGENOM; CLU_100701_0_0_1; -.
DR InParanoid; Q02685; -.
DR OMA; CEYLEAK; -.
DR BioCyc; YEAST:G3O-33941-MON; -.
DR PRO; PR:Q02685; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q02685; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0043596; C:nuclear replication fork; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0031422; C:RecQ family helicase-topoisomerase III complex; IDA:SGD.
DR GO; GO:0008047; F:enzyme activator activity; IDA:SGD.
DR GO; GO:0000400; F:four-way junction DNA binding; IDA:SGD.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:SGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IGI:SGD.
DR GO; GO:0006265; P:DNA topological change; IDA:SGD.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IDA:ComplexPortal.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007064; P:mitotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:0043388; P:positive regulation of DNA binding; IDA:SGD.
DR Gene3D; 2.40.50.770; -; 1.
DR InterPro; IPR013894; RMI1_N.
DR InterPro; IPR042470; RMI1_N_C_sf.
DR Pfam; PF08585; RMI1_N; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; DNA damage; DNA-binding; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..241
FT /note="RecQ-mediated genome instability protein 1"
FT /id="PRO_0000270571"
SQ SEQUENCE 241 AA; 26860 MW; 65EF152F50BF0084 CRC64;
MSFSSILSQD ITDDITPPAY SATLGSREQI VFRAYQNEPW LAGTASNLIL DKKLVIVDRE
LLFQVLMVEN ITKSKLTQID DIKTKLDPKK QKVDRLRSGA QGNGAKKYEV ITQVDMEDDG
NVADNNCAKE NNSNNNSSAA KNKAVFKLTL QSKSGDVFFA INSTPISWSS CMLGSKIVIL
PGTVFNRGVF ILKDSQVIFL GGINRVWNEN RDQKFCDYLE SKLQRDKQLV NGGSKKRKAN
D
