RMI2_CAEEL
ID RMI2_CAEEL Reviewed; 107 AA.
AC Q8MXU4;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=RecQ-mediated genome instability protein 2 homolog {ECO:0000305};
DE AltName: Full=RMI2 functional homolog 2 {ECO:0000303|PubMed:34252074};
GN Name=rmif-2 {ECO:0000303|PubMed:34252074, ECO:0000312|WormBase:Y104H12D.4};
GN ORFNames=Y104H12D.4 {ECO:0000312|WormBase:Y104H12D.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN BTR COMPLEX, INTERACTION WITH RMH-1,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=34252074; DOI=10.1371/journal.pgen.1009663;
RA Velkova M., Silva N., Dello Stritto M.R., Schleiffer A., Barraud P.,
RA Hartl M., Jantsch V.;
RT "Caenorhabditis elegans RMI2 functional homolog-2 (RMIF-2) and RMI1 (RMH-1)
RT have both overlapping and distinct meiotic functions within the BTR
RT complex.";
RL PLoS Genet. 17:e1009663-e1009663(2021).
CC -!- FUNCTION: Essential component of the RMI complex, a complex that plays
CC an important role in the processing of homologous recombination
CC intermediates (By similarity). Component of the BTR double Holliday
CC Junction dissolution complex, which is involved in homologous
CC recombination during meiotic double strand break in the germline
CC (Probable). Plays a role in double strand break repair by positively
CC regulating the accumulation of rad-51 at double strand breaks
CC (PubMed:34252074). Stabilizes and positively regulates the localization
CC of the BTR double Holliday Junction dissolution complex components rmh-
CC 1, him-6 and top-3 at nuclear foci during meiotic recombination
CC (PubMed:34252074). Positively regulates meiotic recombination, chiasma
CC formation, and chromosome segregation in meiosis (PubMed:34252074).
CC Positively regulates DNA crossover formation and positioning on
CC chromosome arms (away from the chromosome center) during homologous
CC recombination (PubMed:34252074). {ECO:0000250|UniProtKB:Q96E14,
CC ECO:0000269|PubMed:34252074, ECO:0000305|PubMed:34252074}.
CC -!- SUBUNIT: Component of the RMI complex, containing at least top-3, rmh-1
CC and rmh-2 (By similarity). Component of the BTR double Holliday
CC Junction dissolution complex composed of at least him-6, top-3, rmh-1
CC and rmif-2, which is involved in double strand break repair in the
CC germline (Probable). Interacts with rmh-1; the interaction is direct
CC and is required for mutual stability and localization at nuclear foci
CC (PubMed:34252074). {ECO:0000250|UniProtKB:Q96E14,
CC ECO:0000269|PubMed:34252074, ECO:0000305|PubMed:34252074}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Note=Co-localizes with rmh-1 in nuclei
CC foci during pachytene in meiotic prophase I (PubMed:34252074).
CC Localization at nuclear foci is dependent on rmh-1 during meiotic
CC recombination (PubMed:34252074). {ECO:0000269|PubMed:34252074}.
CC -!- TISSUE SPECIFICITY: Expressed in the germline.
CC {ECO:0000269|PubMed:34252074}.
CC -!- DEVELOPMENTAL STAGE: First expressed in early pachynema nuclei during
CC meiotic prophase I in the gonad (PubMed:34252074). Expression increases
CC in throughout mid pachynema and decreases in the late stages of
CC pachynema (PubMed:34252074). {ECO:0000269|PubMed:34252074}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality in 40% of animals, reduced
CC brood size and high incidence of males phenotypes (PubMed:34252074).
CC Presence of a mix of univalent and bivalent chromosomes during
CC diakinesis, impaired double strand breaks and chiasma formation and
CC defective chromosome segregation (PubMed:34252074). Delayed double
CC break strand repair with an accumulation of rad-51-positive foci in
CC early/mid pachynema in meiotic prophase I during meiotic recombination
CC (PubMed:34252074). Delayed accumulation of cosa-1-positive and msh-5-
CC positive foci at recombination foci (composed of crossover and non-
CC crossover DNA intermediates) in late pachynema indicative of a
CC decreased efficiency in crossover designation (PubMed:34252074).
CC Increased number of crossover events and impaired positioning of
CC crossovers on chromosome arms during homologous recombination
CC (PubMed:34252074). Animals have an extended mitotic zone
CC (PubMed:34252074). Abolishes localization of rmh-1 to nuclear foci, and
CC instead rmh-1 is localized in the cytoplasm (PubMed:34252074). Impairs
CC the localization of him-6 to nuclear foci during pachynema
CC (PubMed:34252074). {ECO:0000269|PubMed:34252074}.
CC -!- SIMILARITY: Belongs to the RMI2 family. {ECO:0000305}.
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DR EMBL; BX284604; CCD67731.1; -; Genomic_DNA.
DR RefSeq; NP_741302.1; NM_171253.3.
DR AlphaFoldDB; Q8MXU4; -.
DR STRING; 6239.Y104H12D.4; -.
DR PaxDb; Q8MXU4; -.
DR EnsemblMetazoa; Y104H12D.4.1; Y104H12D.4.1; WBGene00022428.
DR UCSC; Y104H12D.4; c. elegans.
DR WormBase; Y104H12D.4; CE31633; WBGene00022428; rmif-2.
DR eggNOG; ENOG502TIES; Eukaryota.
DR HOGENOM; CLU_2199385_0_0_1; -.
DR InParanoid; Q8MXU4; -.
DR OMA; GETIICA; -.
DR OrthoDB; 1831376at2759; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00022428; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051026; P:chiasma assembly; IMP:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0090727; P:positive regulation of brood size; IMP:UniProtKB.
DR GO; GO:0040019; P:positive regulation of embryonic development; IMP:UniProtKB.
DR GO; GO:1905134; P:positive regulation of meiotic chromosome separation; IMP:UniProtKB.
DR GO; GO:0010845; P:positive regulation of reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0034504; P:protein localization to nucleus; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; DNA replication; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..107
FT /note="RecQ-mediated genome instability protein 2 homolog"
FT /id="PRO_0000455413"
SQ SEQUENCE 107 AA; 12006 MW; 0D2A6BC3F18EE10F CRC64;
MCDRDDCASI ASRCTGETII CAFVESITPP KIRDKTGTYE LKCPFAEGFT MEIGDLFLFH
FVFDGSSEKC TRLTPIPKML AMVYEKLLKD LRKAKKEGKM IISPTAN
