RMLC_MYCS2
ID RMLC_MYCS2 Reviewed; 201 AA.
AC A0QSK5; I7FGH4;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=dTDP-4-dehydrorhamnose 3,5-epimerase {ECO:0000305};
DE EC=5.1.3.13 {ECO:0000269|PubMed:16472764};
DE AltName: Full=Thymidine diphospho-4-keto-rhamnose 3,5-epimerase {ECO:0000305};
DE AltName: Full=dTDP-4-keto-6-deoxyglucose 3,5-epimerase {ECO:0000305};
DE AltName: Full=dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase {ECO:0000305};
DE AltName: Full=dTDP-L-rhamnose synthase {ECO:0000305};
GN Name=rmlC; OrderedLocusNames=MSMEG_1510, MSMEI_1475;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS, AND CATALYTIC ACTIVITY.
RX PubMed=16472764; DOI=10.1016/j.bbrc.2006.01.130;
RA Li W., Xin Y., McNeil M.R., Ma Y.;
RT "rmlB and rmlC genes are essential for growth of mycobacteria.";
RL Biochem. Biophys. Res. Commun. 342:170-178(2006).
CC -!- FUNCTION: Catalyzes the epimerization of the C3' and C5'positions of
CC dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.
CC Involved in the biosynthesis of the dTDP-L-rhamnose which is a
CC component of the critical linker, D-N-acetylglucosamine-L-rhamnose
CC disaccharide, which connects the galactan region of arabinogalactan to
CC peptidoglycan via a phosphodiester linkage.
CC {ECO:0000269|PubMed:16472764}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-
CC L-rhamnose; Xref=Rhea:RHEA:16969, ChEBI:CHEBI:57649,
CC ChEBI:CHEBI:62830; EC=5.1.3.13;
CC Evidence={ECO:0000269|PubMed:16472764};
CC -!- PATHWAY: Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
CC {ECO:0000250|UniProtKB:P26394}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P9WH11}.
CC -!- SIMILARITY: Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK71239.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37948.1; -; Genomic_DNA.
DR RefSeq; WP_011727714.1; NZ_SIJM01000016.1.
DR RefSeq; YP_885893.1; NC_008596.1.
DR AlphaFoldDB; A0QSK5; -.
DR SMR; A0QSK5; -.
DR STRING; 246196.MSMEI_1475; -.
DR EnsemblBacteria; ABK71239; ABK71239; MSMEG_1510.
DR EnsemblBacteria; AFP37948; AFP37948; MSMEI_1475.
DR GeneID; 66732968; -.
DR KEGG; msg:MSMEI_1475; -.
DR KEGG; msm:MSMEG_1510; -.
DR PATRIC; fig|246196.19.peg.1496; -.
DR eggNOG; COG1898; Bacteria.
DR OMA; RGVFLEW; -.
DR OrthoDB; 1618609at2; -.
DR UniPathway; UPA00124; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0008830; F:dTDP-4-dehydrorhamnose 3,5-epimerase activity; IGI:UniProtKB.
DR GO; GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IGI:UniProtKB.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR000888; dTDP_sugar_isom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR21047; PTHR21047; 1.
DR Pfam; PF00908; dTDP_sugar_isom; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Isomerase; Reference proteome.
FT CHAIN 1..201
FT /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
FT /id="PRO_0000399899"
FT ACT_SITE 62
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT ACT_SITE 132
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 23
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 28
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 47..49
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 59
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 72
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 166
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT SITE 138
FT /note="Participates in a stacking interaction with the
FT thymidine ring of dTDP-4-oxo-6-deoxyglucose"
FT /evidence="ECO:0000250|UniProtKB:Q5SFD1"
SQ SEQUENCE 201 AA; 22241 MW; 7E3E273F547CBE7F CRC64;
MTARELSIAG AWEITPVLRT DSRGLFFEWF TDAGFTEFAG HQFDMRQANC SVSARGVLRG
VHFAQVPPSQ AKYVTCVRGA VFDVVVDIRV GSPTFGQWDA VLLDDKDRRS IYISEGLGHA
FLALDDDSTV MYLCSAPYAP QREHTVRPTD FGIEWPEVPE LILSDRDAQA PSLAEAQAAG
VLPTWADCQA FVETLRRNLV S
