RMLC_SHIFL
ID RMLC_SHIFL Reviewed; 181 AA.
AC P37780;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=dTDP-4-dehydrorhamnose 3,5-epimerase {ECO:0000250|UniProtKB:P26394};
DE EC=5.1.3.13 {ECO:0000250|UniProtKB:P26394};
DE AltName: Full=Thymidine diphospho-4-keto-rhamnose 3,5-epimerase {ECO:0000250|UniProtKB:P26394};
DE AltName: Full=dTDP-4-keto-6-deoxyglucose 3,5-epimerase {ECO:0000250|UniProtKB:P26394};
DE AltName: Full=dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase {ECO:0000250|UniProtKB:P26394};
DE AltName: Full=dTDP-L-rhamnose synthase {ECO:0000250|UniProtKB:P26394};
GN Name=rfbC; Synonyms=rmlC; OrderedLocusNames=SF2101, S2223;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PE577 / Serotype 2a;
RX PubMed=8170390; DOI=10.1111/j.1365-2958.1994.tb00308.x;
RA Macpherson D.F., Manning P.A., Morona R.;
RT "Characterization of the dTDP-rhamnose biosynthetic genes encoded in the
RT rfb locus of Shigella flexneri.";
RL Mol. Microbiol. 11:281-292(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6200 / Serotype 2a;
RX PubMed=8157605; DOI=10.1128/jb.176.8.2362-2373.1994;
RA Rajakumar K., Jost B.H., Sasakawa C., Okada N., Yoshikawa M., Adler B.;
RT "Nucleotide sequence of the rhamnose biosynthetic operon of Shigella
RT flexneri 2a and role of lipopolysaccharide in virulence.";
RL J. Bacteriol. 176:2362-2373(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Catalyzes the epimerization of the C3' and C5'positions of
CC dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.
CC {ECO:0000250|UniProtKB:P26394}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-
CC L-rhamnose; Xref=Rhea:RHEA:16969, ChEBI:CHEBI:57649,
CC ChEBI:CHEBI:62830; EC=5.1.3.13;
CC Evidence={ECO:0000250|UniProtKB:P26394};
CC -!- PATHWAY: Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
CC {ECO:0000250|UniProtKB:P26394}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC biosynthesis. {ECO:0000250|UniProtKB:P26394}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P26394}.
CC -!- SIMILARITY: Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.
CC {ECO:0000305}.
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DR EMBL; X71970; CAA50770.1; -; Genomic_DNA.
DR EMBL; L14842; AAA53682.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN43640.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP17469.1; -; Genomic_DNA.
DR PIR; E55213; E55213.
DR RefSeq; NP_707933.1; NC_004337.2.
DR RefSeq; WP_001100804.1; NZ_WPGV01000030.1.
DR AlphaFoldDB; P37780; -.
DR SMR; P37780; -.
DR STRING; 198214.SF2101; -.
DR EnsemblBacteria; AAN43640; AAN43640; SF2101.
DR EnsemblBacteria; AAP17469; AAP17469; S2223.
DR GeneID; 1026775; -.
DR KEGG; sfl:SF2101; -.
DR KEGG; sfx:S2223; -.
DR PATRIC; fig|198214.7.peg.2509; -.
DR HOGENOM; CLU_090940_1_1_6; -.
DR OMA; RGVFLEW; -.
DR OrthoDB; 1618609at2; -.
DR UniPathway; UPA00124; -.
DR UniPathway; UPA00281; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0008830; F:dTDP-4-dehydrorhamnose 3,5-epimerase activity; ISS:UniProtKB.
DR GO; GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; ISS:UniProtKB.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; ISS:UniProtKB.
DR GO; GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR000888; dTDP_sugar_isom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR21047; PTHR21047; 1.
DR Pfam; PF00908; dTDP_sugar_isom; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR TIGRFAMs; TIGR01221; rmlC; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Isomerase; Lipopolysaccharide biosynthesis;
KW Reference proteome.
FT CHAIN 1..181
FT /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
FT /id="PRO_0000207980"
FT ACT_SITE 62
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT ACT_SITE 132
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 23
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 28
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 47..49
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 59
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 72
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT BINDING 167
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9HU21"
FT SITE 138
FT /note="Participates in a stacking interaction with the
FT thymidine ring of dTDP-4-oxo-6-deoxyglucose"
FT /evidence="ECO:0000250|UniProtKB:Q5SFD1"
SQ SEQUENCE 181 AA; 20823 MW; 25C1DB7591CDB229 CRC64;
MNVIKTEIPD VLIFEPKVFG DERGFFMESF NQKVFEEAVG RKVEFVQDNH SKSTKGVLRG
LHYQLEPYAQ GKLVRCVVGE VFDVAVDIRK SSPTFGKWVG VNLSAENKRQ LWIPEGFAHG
FCVLSDEAEF VYKTNNFYSK MQERGILWSD KSINIEWPVQ NPLLSDKDIN GQKFVDADYF
I
