RMV1_ARATH
ID RMV1_ARATH Reviewed; 490 AA.
AC Q9FFL1;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Polyamine transporter RMV1;
DE AltName: Full=Protein RESISTANT TO METHYL VIOLOGEN 1;
GN Name=RMV1; OrderedLocusNames=At5g05630; ORFNames=MJJ3.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, MUTAGENESIS OF ILE-377, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=22492932; DOI=10.1073/pnas.1121406109;
RA Fujita M., Fujita Y., Iuchi S., Yamada K., Kobayashi Y., Urano K.,
RA Kobayashi M., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Natural variation in a polyamine transporter determines paraquat tolerance
RT in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:6343-6347(2012).
CC -!- FUNCTION: Cell membrane polyamine/proton symporter involved in the
CC polyamine uptake in cells. Possesses high affinity for spermine and
CC spermidine and lower affinity for putrescine. Transports paraquat, a
CC polyamine analog, and thus confers sensitivity to this chemical which
CC is used as a herbicide. {ECO:0000269|PubMed:22492932}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.6 uM for spermine {ECO:0000269|PubMed:22492932};
CC KM=2.2 uM for spermidine {ECO:0000269|PubMed:22492932};
CC KM=56.5 uM for putrescine {ECO:0000269|PubMed:22492932};
CC KM=24.4 uM for paraquat {ECO:0000269|PubMed:22492932};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:22492932};
CC Multi-pass membrane protein {ECO:0000305|PubMed:22492932}. Note=Plasma
CC membrane.
CC -!- DEVELOPMENTAL STAGE: Expressed in hypocotyls and petioles of cotyledons
CC in 1- to 3-day-old seedlings. {ECO:0000269|PubMed:22492932}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants show increased tolerance to paraquat.
CC {ECO:0000269|PubMed:22492932}.
CC -!- MISCELLANEOUS: Methyl viologen is the brand name of paraquat. Plants
CC over-expressing RMV1 show hypersensitivity to paraquat
CC (PubMed:22492932). {ECO:0000305|PubMed:22492932}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.
CC {ECO:0000305}.
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DR EMBL; AB005237; BAB09657.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90901.1; -; Genomic_DNA.
DR EMBL; AK119108; BAC43680.1; -; mRNA.
DR EMBL; BT008298; AAP37657.1; -; mRNA.
DR RefSeq; NP_196182.1; NM_120645.3.
DR AlphaFoldDB; Q9FFL1; -.
DR SMR; Q9FFL1; -.
DR STRING; 3702.AT5G05630.1; -.
DR TCDB; 2.A.3.12.3; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; Q9FFL1; -.
DR PRIDE; Q9FFL1; -.
DR ProteomicsDB; 228186; -.
DR EnsemblPlants; AT5G05630.1; AT5G05630.1; AT5G05630.
DR GeneID; 830446; -.
DR Gramene; AT5G05630.1; AT5G05630.1; AT5G05630.
DR KEGG; ath:AT5G05630; -.
DR Araport; AT5G05630; -.
DR TAIR; locus:2166354; AT5G05630.
DR eggNOG; KOG1287; Eukaryota.
DR HOGENOM; CLU_007946_17_3_1; -.
DR InParanoid; Q9FFL1; -.
DR OMA; ASLNICH; -.
DR OrthoDB; 621852at2759; -.
DR PhylomeDB; Q9FFL1; -.
DR PRO; PR:Q9FFL1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFL1; baseline and differential.
DR Genevisible; Q9FFL1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015203; F:polyamine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015839; P:cadaverine transport; IMP:TAIR.
DR GO; GO:0015846; P:polyamine transport; IDA:TAIR.
DR GO; GO:0009408; P:response to heat; IMP:TAIR.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR044566; RMV1-like.
DR PANTHER; PTHR45826; PTHR45826; 1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..490
FT /note="Polyamine transporter RMV1"
FT /id="PRO_0000418912"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 377
FT /note="I->F: Loss of sensitivity to paraquat."
FT /evidence="ECO:0000269|PubMed:22492932"
SQ SEQUENCE 490 AA; 53694 MW; E478743784CFE309 CRC64;
MTELSSPNLD SASQKPRIST ENPPPPPPHI SIGVTTGDPA TSPARTVNQI KKITVLPLVF
LIFYEVSGGP FGIEDSVKAA GPLLAIVGFI VFPFIWSIPE ALITAEMGTM FPENGGYVVW
VTLAMGPYWG FQQGWVKWLS GVIDNALYPI LFLDYLKSGI PILGSGIPRV AAILVLTVAL
TYLNYRGLSI VGVAAVLLGV FSILPFVVMS FMSIPKLKPS RWLVVSKKMK GVNWSLYLNT
LFWNLNYWDS VSTLTGEVEN PSKTLPRALF YALLLVVFSY IFPVLTGTGA IALDQKLWTD
GYFADIGKVI GGVWLGWWIQ AAAATSNMGM FLAEMSSDSF QLLGMAERGM LPEVFAKRSR
YRTPWVGILF SASGVIILSW LSFQEIVAAE NLLYCFGMVL EFITFVRLRM KYPAASRPFK
IPVGVLGSVL MCIPPTVLIG VIMAFTNLKV ALVSLAAIVI GLVLQPCLKQ VEKKGWLKFS
TSSHLPNLME
