RN121_HUMAN
ID RN121_HUMAN Reviewed; 327 AA.
AC Q9H920; B3KSW8; Q6IA57; Q6P449; Q96DB4;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=RING finger protein 121;
GN Name=RNF121;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain cortex, and Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-327 (ISOFORM 1).
RC TISSUE=Duodenum, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H920-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H920-2; Sequence=VSP_054133;
CC -!- SIMILARITY: Belongs to the RNF121 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH09672.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH63680.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK023139; BAB14423.1; -; mRNA.
DR EMBL; AK094508; BAG52880.1; -; mRNA.
DR EMBL; CR457298; CAG33579.1; -; mRNA.
DR EMBL; AP002490; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471076; EAW74810.1; -; Genomic_DNA.
DR EMBL; BC009672; AAH09672.2; ALT_INIT; mRNA.
DR EMBL; BC063680; AAH63680.1; ALT_INIT; mRNA.
DR CCDS; CCDS8203.1; -. [Q9H920-1]
DR RefSeq; NP_060790.2; NM_018320.4. [Q9H920-1]
DR RefSeq; XP_006718693.1; XM_006718630.1. [Q9H920-2]
DR RefSeq; XP_011543452.1; XM_011545150.1. [Q9H920-2]
DR AlphaFoldDB; Q9H920; -.
DR BioGRID; 120585; 12.
DR IntAct; Q9H920; 3.
DR STRING; 9606.ENSP00000354571; -.
DR iPTMnet; Q9H920; -.
DR PhosphoSitePlus; Q9H920; -.
DR SwissPalm; Q9H920; -.
DR BioMuta; RNF121; -.
DR CPTAC; CPTAC-1633; -.
DR EPD; Q9H920; -.
DR jPOST; Q9H920; -.
DR MassIVE; Q9H920; -.
DR MaxQB; Q9H920; -.
DR PaxDb; Q9H920; -.
DR PeptideAtlas; Q9H920; -.
DR PRIDE; Q9H920; -.
DR ProteomicsDB; 3656; -.
DR ProteomicsDB; 81272; -. [Q9H920-1]
DR Antibodypedia; 16872; 101 antibodies from 20 providers.
DR DNASU; 55298; -.
DR Ensembl; ENST00000361756.8; ENSP00000354571.3; ENSG00000137522.18. [Q9H920-1]
DR GeneID; 55298; -.
DR KEGG; hsa:55298; -.
DR MANE-Select; ENST00000361756.8; ENSP00000354571.3; NM_018320.5; NP_060790.2.
DR UCSC; uc001ora.4; human. [Q9H920-1]
DR CTD; 55298; -.
DR DisGeNET; 55298; -.
DR GeneCards; RNF121; -.
DR HGNC; HGNC:21070; RNF121.
DR HPA; ENSG00000137522; Low tissue specificity.
DR neXtProt; NX_Q9H920; -.
DR OpenTargets; ENSG00000137522; -.
DR PharmGKB; PA134985388; -.
DR VEuPathDB; HostDB:ENSG00000137522; -.
DR eggNOG; KOG1734; Eukaryota.
DR GeneTree; ENSGT00390000013075; -.
DR InParanoid; Q9H920; -.
DR OMA; HQQHMDL; -.
DR OrthoDB; 1167893at2759; -.
DR PhylomeDB; Q9H920; -.
DR TreeFam; TF314357; -.
DR PathwayCommons; Q9H920; -.
DR SignaLink; Q9H920; -.
DR BioGRID-ORCS; 55298; 32 hits in 1117 CRISPR screens.
DR ChiTaRS; RNF121; human.
DR GenomeRNAi; 55298; -.
DR Pharos; Q9H920; Tbio.
DR PRO; PR:Q9H920; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9H920; protein.
DR Bgee; ENSG00000137522; Expressed in quadriceps femoris and 105 other tissues.
DR ExpressionAtlas; Q9H920; baseline and differential.
DR Genevisible; Q9H920; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR040176; RNF121/RNF175.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR13407; PTHR13407; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Metal-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..327
FT /note="RING finger protein 121"
FT /id="PRO_0000261407"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 226..276
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VAR_SEQ 1..81
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054133"
FT CONFLICT 327
FT /note="E -> D (in Ref. 2; CAG33579)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 327 AA; 37882 MW; FFC170FF52AB15CD CRC64;
MAAVVEVEVG GGAAGERELD EVDMSDLSPE EQWRVEHARM HAKHRGHEAM HAEMVLILIA
TLVVAQLLLV QWKQRHPRSY NMVTLFQMWV VPLYFTVKLH WWRFLVIWIL FSAVTAFVTF
RATRKPLVQT TPRLVYKWFL LIYKISYATG IVGYMAVMFT LFGLNLLFKI KPEDAMDFGI
SLLFYGLYYG VLERDFAEMC ADYMASTIGF YSESGMPTKH LSDSVCAVCG QQIFVDVSEE
GIIENTYRLS CNHVFHEFCI RGWCIVGKKQ TCPYCKEKVD LKRMFSNPWE RPHVMYGQLL
DWLRYLVAWQ PVIIGVVQGI NYILGLE
