RN150_MOUSE
ID RN150_MOUSE Reviewed; 437 AA.
AC Q5DTZ6;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=RING finger protein 150;
DE Flags: Precursor;
GN Name=Rnf150; Synonyms=Kiaa1214;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5DTZ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5DTZ6-2; Sequence=VSP_023848;
CC Name=3;
CC IsoId=Q5DTZ6-3; Sequence=VSP_023849, VSP_023850;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90433.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK220374; BAD90433.1; ALT_INIT; mRNA.
DR EMBL; AK041412; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC124757; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC132372; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC166939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40402.1; -. [Q5DTZ6-1]
DR RefSeq; NP_796352.2; NM_177378.4. [Q5DTZ6-1]
DR AlphaFoldDB; Q5DTZ6; -.
DR SMR; Q5DTZ6; -.
DR STRING; 10090.ENSMUSP00000077610; -.
DR GlyGen; Q5DTZ6; 4 sites.
DR PhosphoSitePlus; Q5DTZ6; -.
DR MaxQB; Q5DTZ6; -.
DR PaxDb; Q5DTZ6; -.
DR PRIDE; Q5DTZ6; -.
DR ProteomicsDB; 299918; -. [Q5DTZ6-1]
DR ProteomicsDB; 299919; -. [Q5DTZ6-2]
DR ProteomicsDB; 299920; -. [Q5DTZ6-3]
DR Antibodypedia; 27282; 153 antibodies from 22 providers.
DR DNASU; 330812; -.
DR Ensembl; ENSMUST00000078525; ENSMUSP00000077610; ENSMUSG00000047747. [Q5DTZ6-1]
DR GeneID; 330812; -.
DR KEGG; mmu:330812; -.
DR UCSC; uc009mjt.2; mouse. [Q5DTZ6-1]
DR UCSC; uc029wub.2; mouse. [Q5DTZ6-3]
DR CTD; 57484; -.
DR MGI; MGI:2443860; Rnf150.
DR VEuPathDB; HostDB:ENSMUSG00000047747; -.
DR eggNOG; KOG0800; Eukaryota.
DR GeneTree; ENSGT00940000156171; -.
DR HOGENOM; CLU_049885_2_1_1; -.
DR InParanoid; Q5DTZ6; -.
DR OMA; VNITYMD; -.
DR OrthoDB; 1487241at2759; -.
DR PhylomeDB; Q5DTZ6; -.
DR TreeFam; TF317486; -.
DR BioGRID-ORCS; 330812; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Rnf150; mouse.
DR PRO; PR:Q5DTZ6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q5DTZ6; protein.
DR Bgee; ENSMUSG00000047747; Expressed in pigmented layer of retina and 196 other tissues.
DR Genevisible; Q5DTZ6; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Metal-binding;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Zinc;
KW Zinc-finger.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..437
FT /note="RING finger protein 150"
FT /id="PRO_0000280698"
FT TOPO_DOM 35..207
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 80..182
FT /note="PA"
FT ZN_FING 277..318
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 152
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 185
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023848"
FT VAR_SEQ 245..286
FT /note="RRLGDAAKKAISKLQVRTIRKGDKETESDFDNCAVCIEGYKP -> VSSSLR
FT KAWLCKLSANHTCQGINVVAHKSRDSKCLLTPPHTC (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023849"
FT VAR_SEQ 287..437
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023850"
FT CONFLICT 108
FT /note="A -> V (in Ref. 1; BAD90433)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="E -> K (in Ref. 2; AK041412)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 437 AA; 48013 MW; 148A94349EDD2CD9 CRC64;
MTMSLIQACR SLALSTWLLS FCFVHLLCLD FTVAEKEEWY TAFVNITYLE PEPGAAVAGS
GGGAELHTEK SECGRYGEHS PKQDARGEVV MASSAQDRLA CDPNTKFAAP AHGKHWIALI
PKGNCTYRDK IRNAFLQNAS AVVIFNVGSN TNETITMPHA GVEDIVAIMI PEPKGKEIVS
LLERNITVTM YITIGTRNLQ KYVSRTSVVF VSISFIVLMI ISLAWLVFYY IQRFRYANAR
DRNQRRLGDA AKKAISKLQV RTIRKGDKET ESDFDNCAVC IEGYKPNDVV RILPCRHLFH
KSCVDPWLLD HRTCPMCKMN ILKALGIPPN ADCMDDLPID FEGSLGGPPT NQITGASDTT
VNESSVTLDP AVRTVGALQV VQDPDPAPQE GEAIFTTNSG QEPALSSDSD ISLIMALEVG
LSDVELSTDQ DCEEVKS
