ID   RN182_XENLA             Reviewed;         246 AA.
AC   Q6INB3;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=E3 ubiquitin-protein ligase RNF182;
DE            EC=2.3.2.27;
DE   AltName: Full=RING finger protein 182;
DE   AltName: Full=RING-type E3 ubiquitin transferase RNF182 {ECO:0000305};
GN   Name=rnf182;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase that mediates the ubiquitination
CC       of atp6v0c and targets it to degradation via the ubiquitin-proteasome
CC       pathway. {ECO:0000250|UniProtKB:Q8N6D2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with ATP6V0C. {ECO:0000250|UniProtKB:Q8N6D2}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}. Cytoplasm {ECO:0000250|UniProtKB:Q8N6D2}.
CC   -!- DOMAIN: The RING-type zinc finger domain is required for E3 ligase
CC       activity. {ECO:0000250}.
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DR   EMBL; BC072370; AAH72370.1; -; mRNA.
DR   RefSeq; NP_001085077.1; NM_001091608.1.
DR   AlphaFoldDB; Q6INB3; -.
DR   SMR; Q6INB3; -.
DR   PRIDE; Q6INB3; -.
DR   DNASU; 432148; -.
DR   GeneID; 432148; -.
DR   KEGG; xla:432148; -.
DR   CTD; 432148; -.
DR   Xenbase; XB-GENE-17336205; rnf182.S.
DR   OrthoDB; 1216224at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Chromosome 6S.
DR   Bgee; 432148; Expressed in camera-type eye and 1 other tissue.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR042285; RNF182.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   PANTHER; PTHR46675; PTHR46675; 1.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Membrane; Metal-binding; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix; Ubl conjugation pathway; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..246
FT                   /note="E3 ubiquitin-protein ligase RNF182"
FT                   /id="PRO_0000261622"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ZN_FING         22..70
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ   SEQUENCE   246 AA;  27604 MW;  BFFDA2F535460856 CRC64;
     MTSQLSEDNS ESPNLNSDEL ECKICYNRYN LRQRKPKVLG CCHRVCAKCL YKLVDCGESP
     QCVIVCPFCR FETRMPEDEV SSLPDDNNIL LNLACGGRGK CVGDNPTELL LTPKRLSTIV
     TPSHTSTNCL VITIMEVQRE SSPALNTTPM VEFYRPSNYD PVSIPQNWTV WNCTSLICKT
     SVRVFVWLLG LLYFSSLPLG IYLLVSKKVT LGVVFVSLVP SSLVILMIYG FCQCMCHEFL
     DCMSTP