ID   RN1T_RANTE              Reviewed;          33 AA.
AC   P82740; P82236;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Ranatuerin-1T;
DE   AltName: Full=Brevinin-2T;
OS   Rana temporaria (European common frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=8407;
RN   [1]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Skin secretion;
RX   PubMed=10333736;
RX   DOI=10.1002/(sici)1097-0282(1998)47:6<435::aid-bip3>3.0.co;2-8;
RA   Simmaco M., Mignogna G., Barra D.;
RT   "Antimicrobial peptides from amphibian skin: what do they tell us?";
RL   Biopolymers 47:435-450(1998).
RN   [2]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=10422869; DOI=10.1016/s0196-9781(98)00174-0;
RA   Goraya J., Knoop F.C., Conlon J.M.;
RT   "Ranatuerin 1T: an antimicrobial peptide isolated from the skin of the
RT   frog, Rana temporaria.";
RL   Peptides 20:159-163(1999).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus and Gram-negative bacterium E.coli. No activity against
CC       C.albicans. {ECO:0000269|PubMed:10333736, ECO:0000269|PubMed:10422869}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=3397.3; Mass_error=0.02; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10422869};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82740; -.
DR   SMR; P82740; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..33
FT                   /note="Ranatuerin-1T"
FT                   /id="PRO_0000044661"
FT   DISULFID        27..33
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   33 AA;  3400 MW;  CCE5C1C4D37FB41B CRC64;
     GLLSGLKKVG KHVAKNVAVS LMDSLKCKIS GDC