RN214_HUMAN
ID RN214_HUMAN Reviewed; 703 AA.
AC Q8ND24; B2RUW0; B4DTD1;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=RING finger protein 214;
GN Name=RNF214;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 472-703 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-497 AND SER-511, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-40; SER-47; SER-54
RP AND SER-176, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8ND24-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8ND24-2; Sequence=VSP_054566;
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DR EMBL; AK298923; BAG61028.1; -; mRNA.
DR EMBL; AK300160; BAG61943.1; -; mRNA.
DR EMBL; AP000892; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471065; EAW67306.1; -; Genomic_DNA.
DR EMBL; BC031347; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC146891; AAI46892.1; -; mRNA.
DR EMBL; BC146901; AAI46902.1; -; mRNA.
DR EMBL; BC064581; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AL834448; CAD39108.1; -; mRNA.
DR CCDS; CCDS41720.1; -. [Q8ND24-1]
DR CCDS; CCDS60976.1; -. [Q8ND24-2]
DR RefSeq; NP_001070707.1; NM_001077239.1. [Q8ND24-1]
DR RefSeq; NP_001265178.1; NM_001278249.1. [Q8ND24-2]
DR RefSeq; NP_997226.2; NM_207343.3. [Q8ND24-1]
DR AlphaFoldDB; Q8ND24; -.
DR SMR; Q8ND24; -.
DR BioGRID; 129202; 98.
DR IntAct; Q8ND24; 8.
DR STRING; 9606.ENSP00000300650; -.
DR iPTMnet; Q8ND24; -.
DR PhosphoSitePlus; Q8ND24; -.
DR BioMuta; RNF214; -.
DR DMDM; 134035028; -.
DR EPD; Q8ND24; -.
DR jPOST; Q8ND24; -.
DR MassIVE; Q8ND24; -.
DR MaxQB; Q8ND24; -.
DR PaxDb; Q8ND24; -.
DR PeptideAtlas; Q8ND24; -.
DR PRIDE; Q8ND24; -.
DR ProteomicsDB; 5098; -.
DR ProteomicsDB; 72975; -. [Q8ND24-1]
DR Antibodypedia; 32345; 37 antibodies from 14 providers.
DR DNASU; 257160; -.
DR Ensembl; ENST00000300650.9; ENSP00000300650.4; ENSG00000167257.11. [Q8ND24-1]
DR Ensembl; ENST00000531287.5; ENSP00000435361.1; ENSG00000167257.11. [Q8ND24-2]
DR Ensembl; ENST00000531452.5; ENSP00000431643.1; ENSG00000167257.11. [Q8ND24-1]
DR GeneID; 257160; -.
DR KEGG; hsa:257160; -.
DR MANE-Select; ENST00000300650.9; ENSP00000300650.4; NM_207343.4; NP_997226.2.
DR UCSC; uc001pqt.5; human. [Q8ND24-1]
DR CTD; 257160; -.
DR DisGeNET; 257160; -.
DR GeneCards; RNF214; -.
DR HGNC; HGNC:25335; RNF214.
DR HPA; ENSG00000167257; Low tissue specificity.
DR neXtProt; NX_Q8ND24; -.
DR OpenTargets; ENSG00000167257; -.
DR PharmGKB; PA162401772; -.
DR VEuPathDB; HostDB:ENSG00000167257; -.
DR eggNOG; ENOG502QTPR; Eukaryota.
DR GeneTree; ENSGT00940000159470; -.
DR InParanoid; Q8ND24; -.
DR OMA; QEMTSEK; -.
DR PhylomeDB; Q8ND24; -.
DR TreeFam; TF333981; -.
DR PathwayCommons; Q8ND24; -.
DR SignaLink; Q8ND24; -.
DR BioGRID-ORCS; 257160; 29 hits in 1125 CRISPR screens.
DR ChiTaRS; RNF214; human.
DR GenomeRNAi; 257160; -.
DR Pharos; Q8ND24; Tdark.
DR PRO; PR:Q8ND24; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q8ND24; protein.
DR Bgee; ENSG00000167257; Expressed in body of pancreas and 125 other tissues.
DR ExpressionAtlas; Q8ND24; baseline and differential.
DR Genevisible; Q8ND24; HS.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Metal-binding;
KW Phosphoprotein; Reference proteome; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330"
FT CHAIN 2..703
FT /note="RING finger protein 214"
FT /id="PRO_0000280546"
FT ZN_FING 658..700
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 486..552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 220..379
FT /evidence="ECO:0000255"
FT COMPBIAS 37..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 488..507
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT MOD_RES 15
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 40
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163"
FT MOD_RES 54
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 176
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 196
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BFU3"
FT MOD_RES 497
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 511
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 516
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT VAR_SEQ 52..206
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054566"
SQ SEQUENCE 703 AA; 77667 MW; F19F79FD8EECB150 CRC64;
MAASEVAGVV ANAPSPPESS SLCASKSDEG LPDGLSTKDS AQKQKNSPLL SVSSQTITKE
NNRNVHLEHS EQNPGSSAGD TSAAHQVVLG ENLIATALCL SGSGSQSDLK DVASTAGEEG
DTSLRESLHP VTRSLKAGCH TKQLASRNCS EEKSPQTSIL KEGNRDTSLD FRPVVSPANG
VEGVRVDQDD DQDSSSLKLS QNIAVQTDFK TADSEVNTDQ DIEKNLDKMM TERTLLKERY
QEVLDKQRQV ENQLQVQLKQ LQQRREEEMK NHQEILKAIQ DVTIKREETK KKIEKEKKEF
LQKEQDLKAE IEKLCEKGRR EVWEMELDRL KNQDGEINRN IMEETERAWK AEILSLESRK
ELLVLKLEEA EKEAELHLTY LKSTPPTLET VRSKQEWETR LNGVRIMKKN VRDQFNSHIQ
LVRNGAKLSS LPQIPTPTLP PPPSETDFML QVFQPSPSLA PRMPFSIGQV TMPMVMPSAD
PRSLSFPILN PALSQPSQPS SPLPGSHGRN SPGLGSLVSP HGPHMPPAAS IPPPPGLGGV
KASAETPRPQ PVDKLEKILE KLLTRFPQCN KAQMTNILQQ IKTARTTMAG LTMEELIQLV
AARLAEHERV AASTQPLGRI RALFPAPLAQ ISTPMFLPSA QVSYPGRSSH APATCKLCLM
CQKLVQPSEL HPMACTHVLH KECIKFWAQT NTNDTCPFCP TLK
