ID   RN2BA_RANBO             Reviewed;          32 AA.
AC   P84114;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Ranatuerin-2BYa;
OS   Rana boylii (Foothill yellow-legged frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=160499;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:14531844};
RX   PubMed=14531844; DOI=10.1034/j.1399-3011.2003.00090.x;
RA   Conlon J.M., Sonnevend A., Patel M., Davidson C., Nielsen P.F., Pal T.,
RA   Rollins-Smith L.A.;
RT   "Isolation of peptides of the brevinin-1 family with potent candidacidal
RT   activity from the skin secretions of the frog Rana boylii.";
RL   J. Pept. Res. 62:207-213(2003).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus and Gram-negative bacterium E.coli. Weak hemolytic activity.
CC       {ECO:0000269|PubMed:14531844}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14531844}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=3306.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14531844};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000269|PubMed:14531844}.
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DR   AlphaFoldDB; P84114; -.
DR   SMR; P84114; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Hemolysis; Secreted.
FT   PEPTIDE         1..32
FT                   /note="Ranatuerin-2BYa"
FT                   /id="PRO_0000044655"
FT   DISULFID        27..32
FT                   /evidence="ECO:0000269|PubMed:14531844"
SQ   SEQUENCE   32 AA;  3311 MW;  51AE6734F570DF51 CRC64;
     GILSTFKGLA KGVAKDLAGN LLDKFKCKIT GC