ID   RN2CA_LITCL             Reviewed;          31 AA.
AC   P82878;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Ranatuerin-2Ca {ECO:0000303|PubMed:10822101};
OS   Lithobates clamitans (Green frog) (Rana clamitans).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=145282;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=10822101; DOI=10.1016/s0196-9781(00)00178-9;
RA   Halverson T., Basir Y.J., Knoop F.C., Conlon J.M.;
RT   "Purification and characterization of antimicrobial peptides from the skin
RT   of the North American green frog Rana clamitans.";
RL   Peptides 21:469-476(2000).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus and Gram-negative bacterium E.coli. Has activity against
CC       C.albicans. {ECO:0000250|UniProtKB:P82879}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10822101}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:10822101}.
CC   -!- MASS SPECTROMETRY: Mass=3156.4; Mass_error=0.02; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10822101};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P82878; -.
DR   SMR; P82878; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IEA:UniProt.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..31
FT                   /note="Ranatuerin-2Ca"
FT                   /evidence="ECO:0000269|PubMed:10822101"
FT                   /id="PRO_0000044658"
FT   DISULFID        24..29
FT                   /evidence="ECO:0000269|PubMed:10822101"
SQ   SEQUENCE   31 AA;  3159 MW;  79DC2D856D32D2E0 CRC64;
     GLFLDTLKGA AKDVAGKLLE GLKCKIAGCK P