ID   RN2CB_LITCL             Reviewed;          27 AA.
AC   P82879;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Ranatuerin-2Cb {ECO:0000303|PubMed:10822101};
OS   Lithobates clamitans (Green frog) (Rana clamitans).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=145282;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=10822101; DOI=10.1016/s0196-9781(00)00178-9;
RA   Halverson T., Basir Y.J., Knoop F.C., Conlon J.M.;
RT   "Purification and characterization of antimicrobial peptides from the skin
RT   of the North American green frog Rana clamitans.";
RL   Peptides 21:469-476(2000).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus (MIC=40 uM) and Gram-negative bacterium E.coli (MIC=2 uM). Has
CC       activity against C.albicans (MIC=46 uM). {ECO:0000269|PubMed:10822101}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10822101}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:10822101}.
CC   -!- MASS SPECTROMETRY: Mass=2784.0; Mass_error=0.02; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10822101};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82879; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..27
FT                   /note="Ranatuerin-2Cb"
FT                   /evidence="ECO:0000269|PubMed:10822101"
FT                   /id="PRO_0000043568"
FT   DISULFID        20..25
FT                   /evidence="ECO:0000269|PubMed:10822101"
SQ   SEQUENCE   27 AA;  2786 MW;  9912DD7904E723A0 CRC64;
     GLFLDTLKGL AGKLLQGLKC IKAGCKP