ID   RNAM_PLEOS              Reviewed;          12 AA.
AC   P84867;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   11-DEC-2019, entry version 15.
DE   RecName: Full=Ribonuclease;
DE            EC=3.1.-.-;
DE   Flags: Fragment;
OS   Pleurotus ostreatus (Oyster mushroom) (White-rot fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=5322;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND MASS SPECTROMETRY.
RC   TISSUE=Fruiting body {ECO:0000269|PubMed:15946190};
RX   PubMed=15946190; DOI=10.1111/j.1399-3011.2005.00266.x;
RA   Xia L., Chu K.T., Ng T.B.;
RT   "A low-molecular mass ribonuclease from the brown oyster mushroom.";
RL   J. Pept. Res. 66:1-8(2005).
CC   -!- FUNCTION: Has ribonuclease activity towards yeast tRNA, polyadenine,
CC       polycytosine and polyguanine, but lacks ribonuclease activity against
CC       polyuracil. Has antiproliferative activity towards L1210 tumor cell
CC       line with an IC(50) of 41 uM. Lacks mitogenic activity towards murine
CC       splenocytes and does not inhibit HIV-1 reverse transcriptase.
CC       {ECO:0000269|PubMed:15946190}.
CC   -!- ACTIVITY REGULATION: Activated by Mg(2+) and Zn(2+) ions at low
CC       concentrations. Inhibited by Ca(2+), Cu(2+), Fe(2+), Mg(2+), Mn(2+) and
CC       Zn(2+) at higher concentrations. {ECO:0000269|PubMed:15946190}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=60 uM for yeast tRNA {ECO:0000269|PubMed:15946190};
CC       pH dependence:
CC         Optimum pH is 8. Active from pH 3 to 9.
CC         {ECO:0000269|PubMed:15946190};
CC       Temperature dependence:
CC         Optimum temperature is 50-70 degrees Celsius.
CC         {ECO:0000269|PubMed:15946190};
CC   -!- MASS SPECTROMETRY: Mass=8371; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15946190};
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DR   GO; GO:0004540; F:ribonuclease activity; IDA:UniProtKB.
DR   GO; GO:0045839; P:negative regulation of mitotic nuclear division; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..>12
FT                   /note="Ribonuclease"
FT                   /id="PRO_0000244520"
FT   NON_TER         12
FT                   /evidence="ECO:0000303|PubMed:15946190"
SQ   SEQUENCE   12 AA;  1481 MW;  43CDECD3D7CDC2C7 CRC64;
     VRPYLVAFYE SH