ID   RNAS2_PIG               Reviewed;          20 AA.
AC   P80551;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Non-secretory ribonuclease;
DE            EC=4.6.1.18 {ECO:0000250|UniProtKB:P47784};
DE   AltName: Full=Eosinophil-derived neurotoxin;
DE   Flags: Fragment;
GN   Name=RNASE2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RX   PubMed=8645990; DOI=10.1159/000237277;
RA   Fornhem C., Peterson C.G.B., Alving K.;
RT   "Isolation and characterization of porcine cationic eosinophil granule
RT   proteins.";
RL   Int. Arch. Allergy Immunol. 110:132-142(1996).
CC   -!- FUNCTION: This is a non-secretory ribonuclease. It is a pyrimidine
CC       specific nuclease with a slight preference for U. Cytotoxin and
CC       helminthotoxin. Possesses a wide variety of biological activities (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-
CC         phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA].; EC=4.6.1.18;
CC         Evidence={ECO:0000250|UniProtKB:P47784};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-
CC         phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA].; EC=4.6.1.18;
CC         Evidence={ECO:0000250|UniProtKB:P47784};
CC   -!- SUBUNIT: Interacts with and forms a tight 1:1 complex with RNH1.
CC       Dimerization of two such complexes may occur (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000305|PubMed:8645990}.
CC       Cytoplasmic granule {ECO:0000269|PubMed:8645990}. Note=Matrix of
CC       eosinophil's large specific granule.
CC   -!- SIMILARITY: Belongs to the pancreatic ribonuclease family.
CC       {ECO:0000305}.
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DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004522; F:ribonuclease A activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Lyase; Lysosome;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..>20
FT                   /note="Non-secretory ribonuclease"
FT                   /id="PRO_0000057160"
FT   ACT_SITE        16
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   NON_TER         20
SQ   SEQUENCE   20 AA;  2145 MW;  2CD0A6E1ABAE8A6A CRC64;
     VPPGGLTXAQ XFTIQHIXMT