ID   RNAS4_RAT               Reviewed;         147 AA.
AC   O55004;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Ribonuclease 4;
DE            Short=RNase 4;
DE            EC=3.1.27.-;
DE   AltName: Full=RL3;
DE   Flags: Precursor;
GN   Name=Rnase4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=9602056; DOI=10.1016/s0167-4838(97)00213-6;
RA   Zhao W., Kote-Jarai Z., van Santen Y., Hofsteenge J., Beintema J.J.;
RT   "Ribonucleases from rat and bovine liver: purification, specificity and
RT   structural characterization.";
RL   Biochim. Biophys. Acta 1384:55-65(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: This RNase has marked specificity towards the 3' side of
CC       uridine nucleotides.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the pancreatic ribonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; AF041066; AAC23487.1; -; mRNA.
DR   EMBL; BC070953; AAH70953.1; -; mRNA.
DR   RefSeq; NP_064467.1; NM_020082.2.
DR   RefSeq; XP_006251968.1; XM_006251906.2.
DR   RefSeq; XP_006251969.1; XM_006251907.3.
DR   AlphaFoldDB; O55004; -.
DR   SMR; O55004; -.
DR   STRING; 10116.ENSRNOP00000043003; -.
DR   jPOST; O55004; -.
DR   PaxDb; O55004; -.
DR   PRIDE; O55004; -.
DR   Ensembl; ENSRNOT00000041495; ENSRNOP00000043003; ENSRNOG00000025625.
DR   Ensembl; ENSRNOT00000089631; ENSRNOP00000073131; ENSRNOG00000025625.
DR   GeneID; 56759; -.
DR   KEGG; rno:56759; -.
DR   UCSC; RGD:61823; rat.
DR   CTD; 6038; -.
DR   RGD; 61823; Rnase4.
DR   eggNOG; ENOG502S9Q1; Eukaryota.
DR   GeneTree; ENSGT00940000157645; -.
DR   HOGENOM; CLU_117006_3_1_1; -.
DR   InParanoid; O55004; -.
DR   OMA; VPNCRYR; -.
DR   OrthoDB; 1549558at2759; -.
DR   PhylomeDB; O55004; -.
DR   TreeFam; TF333393; -.
DR   PRO; PR:O55004; -.
DR   Proteomes; UP000002494; Chromosome 15.
DR   Bgee; ENSRNOG00000025625; Expressed in liver and 18 other tissues.
DR   ExpressionAtlas; O55004; baseline and differential.
DR   Genevisible; O55004; RN.
DR   GO; GO:0005576; C:extracellular region; ISO:RGD.
DR   GO; GO:0005615; C:extracellular space; ISO:RGD.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0004540; F:ribonuclease activity; ISO:RGD.
DR   GO; GO:0009267; P:cellular response to starvation; ISO:RGD.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR   GO; GO:0090501; P:RNA phosphodiester bond hydrolysis; ISO:RGD.
DR   Gene3D; 3.10.130.10; -; 1.
DR   InterPro; IPR001427; RNaseA.
DR   InterPro; IPR036816; RNaseA-like_dom_sf.
DR   InterPro; IPR023411; RNaseA_AS.
DR   InterPro; IPR023412; RNaseA_domain.
DR   PANTHER; PTHR11437; PTHR11437; 1.
DR   Pfam; PF00074; RnaseA; 1.
DR   PRINTS; PR00794; RIBONUCLEASE.
DR   SMART; SM00092; RNAse_Pc; 1.
DR   SUPFAM; SSF54076; SSF54076; 1.
DR   PROSITE; PS00127; RNASE_PANCREATIC; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Endonuclease; Hydrolase;
KW   Nuclease; Pyrrolidone carboxylic acid; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000250"
FT   CHAIN           29..147
FT                   /note="Ribonuclease 4"
FT                   /id="PRO_0000030887"
FT   ACT_SITE        40
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        144
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         38
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         68..72
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         93
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         110
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         29
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P15467"
FT   DISULFID        53..109
FT                   /evidence="ECO:0000250|UniProtKB:P34096"
FT   DISULFID        67..120
FT                   /evidence="ECO:0000250|UniProtKB:P34096"
FT   DISULFID        85..135
FT                   /evidence="ECO:0000250|UniProtKB:P34096"
FT   DISULFID        92..99
FT                   /evidence="ECO:0000250|UniProtKB:P34096"
SQ   SEQUENCE   147 AA;  16903 MW;  110D54CF7C691BB3 CRC64;
     MDIQRTQSLL LLLLLTLLGL GLVQPSYGQD RMYQRFLRQH VDPEGTGGSD NYCNVMMQRR
     RMTSTQCKRF NTFIHEDIWN IRSICDTANI PCKNGNMNCH EGIVRVTDCR ETGSSVPHNC
     RYRARASTRR VVIACEGTPE VPVHFDR