ID   RNAS8_SAGOE             Reviewed;         155 AA.
AC   Q8SPZ4;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Ribonuclease 8;
DE            Short=RNase 8;
DE            EC=3.1.27.-;
DE   Flags: Precursor;
GN   Name=RNASE8;
OS   Saguinus oedipus (Cotton-top tamarin).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini; Cebidae;
OC   Callitrichinae; Saguinus.
OX   NCBI_TaxID=9490;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11861908; DOI=10.1093/nar/30.5.1169;
RA   Zhang J., Dyer K.D., Rosenberg H.F.;
RT   "RNase 8, a novel RNase A superfamily ribonuclease expressed uniquely in
RT   placenta.";
RL   Nucleic Acids Res. 30:1169-1175(2002).
CC   -!- FUNCTION: Has a low ribonuclease activity. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the pancreatic ribonuclease family.
CC       {ECO:0000305}.
CC   -!- CAUTION: May have lost its catalytic activity. {ECO:0000305}.
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DR   EMBL; AF473861; AAL89650.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8SPZ4; -.
DR   SMR; Q8SPZ4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.10.130.10; -; 1.
DR   InterPro; IPR001427; RNaseA.
DR   InterPro; IPR036816; RNaseA-like_dom_sf.
DR   InterPro; IPR023411; RNaseA_AS.
DR   InterPro; IPR023412; RNaseA_domain.
DR   PANTHER; PTHR11437; PTHR11437; 1.
DR   Pfam; PF00074; RnaseA; 1.
DR   PRINTS; PR00794; RIBONUCLEASE.
DR   SMART; SM00092; RNAse_Pc; 1.
DR   SUPFAM; SSF54076; SSF54076; 1.
DR   PROSITE; PS00127; RNASE_PANCREATIC; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Endonuclease; Hydrolase; Nuclease; Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..155
FT                   /note="Ribonuclease 8"
FT                   /id="PRO_0000030907"
FT   ACT_SITE        150
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         66..70
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         91
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            43
FT                   /note="Ancestral active site"
FT   DISULFID        65..119
FT                   /evidence="ECO:0000250"
FT   DISULFID        83..134
FT                   /evidence="ECO:0000250"
FT   DISULFID        90..97
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   155 AA;  17295 MW;  386BCF955F9DF444 CRC64;
     MAPARAGCCP LLLLLLLGLW VAEIPVSAKP ESMTPSQWFK TQDVQPSPQA CDSAMRNINN
     YKKWCKDLNT FLHEPFSSVA ATCQTPNITC KNGHKNCHQS HRPVSLTMCG LISGKYLNCK
     YKEEHQNKSY IVACDPPQQG DPEYPLVPVH LDKVV