ID   RNB_PHOLL               Reviewed;         647 AA.
AC   Q7N4F5;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Exoribonuclease 2 {ECO:0000255|HAMAP-Rule:MF_01036};
DE            EC=3.1.13.1 {ECO:0000255|HAMAP-Rule:MF_01036};
DE   AltName: Full=Exoribonuclease II {ECO:0000255|HAMAP-Rule:MF_01036};
DE            Short=RNase II {ECO:0000255|HAMAP-Rule:MF_01036};
DE            Short=Ribonuclease II {ECO:0000255|HAMAP-Rule:MF_01036};
GN   Name=rnb {ECO:0000255|HAMAP-Rule:MF_01036}; OrderedLocusNames=plu2384;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS   TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA   Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA   Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Involved in mRNA degradation. Hydrolyzes single-stranded
CC       polyribonucleotides processively in the 3' to 5' direction.
CC       {ECO:0000255|HAMAP-Rule:MF_01036}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01036};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01036}.
CC   -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase II subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01036}.
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DR   EMBL; BX571867; CAE14708.1; -; Genomic_DNA.
DR   RefSeq; WP_011146619.1; NC_005126.1.
DR   AlphaFoldDB; Q7N4F5; -.
DR   SMR; Q7N4F5; -.
DR   STRING; 243265.plu2384; -.
DR   EnsemblBacteria; CAE14708; CAE14708; plu2384.
DR   GeneID; 24166267; -.
DR   KEGG; plu:plu2384; -.
DR   eggNOG; COG4776; Bacteria.
DR   HOGENOM; CLU_002333_7_3_6; -.
DR   OMA; CFTNYLP; -.
DR   OrthoDB; 1602988at2; -.
DR   BioCyc; PLUM243265:PLU_RS11845-MON; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 2.
DR   HAMAP; MF_01036; RNase_II; 1.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR040476; CSD2.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR013223; RNase_B_OB_dom.
DR   InterPro; IPR011804; RNase_II.
DR   InterPro; IPR001900; RNase_II/R.
DR   InterPro; IPR022966; RNase_II/R_CS.
DR   InterPro; IPR004476; RNase_II/RNase_R.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   Pfam; PF17876; CSD2; 1.
DR   Pfam; PF08206; OB_RNB; 1.
DR   Pfam; PF00773; RNB; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00357; CSP; 1.
DR   SMART; SM00955; RNB; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 4.
DR   TIGRFAMs; TIGR00358; 3_prime_RNase; 1.
DR   TIGRFAMs; TIGR02062; RNase_B; 1.
DR   PROSITE; PS01175; RIBONUCLEASE_II; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..647
FT                   /note="Exoribonuclease 2"
FT                   /id="PRO_0000166386"
FT   DOMAIN          564..646
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01036"
SQ   SEQUENCE   647 AA;  74010 MW;  A2EFC2A461711FD6 CRC64;
     MFQNNPLLAQ LKQQLHSQTL RVEGLVKGTE KGFGFLEVDG QKSYFIPPPH MKKVMHGDRV
     TAAIHTDKER EIAEPETLIE PFLNRFVGRI QKKENDNRLW ITPDHPLLKD AIPCRPTNQV
     THPFQHGDWA VAEMRHHPLK GSRGFHAEIT GYITESDNHY APWWVTLTRH NLERDAPAMT
     ADCQMNDGDL ERIDLTSLDF VTIDSATTED MDDALHIAKQ DDGSLKLSIA IADPTAYIAA
     GSELDQIAHQ RAFTNYLPGF NIPMLPRDLS ENLCSLRPNA RRPALVCQVS ILEDGQLGDD
     IAFFSTWVES KAKLVYDEVS DWLEKTGTWE PASEAIKTQI TLLKEMSDRR NQWRHQNALI
     FKDRPDYRFI LDENGYVLDI VVEQRRTANR IVEEAMITSN LCAAKILRDK LGFGIYNVHM
     GFEPLQIEQV VELLQENDID AKTEELLTLD GFCKLRRELD KKPTQFLDSR IRRFQTFAEI
     KPEPGPHFGL GFDAYATWTS PIRKYSDMIN HRLLKAIIQK TDVEQPSEET CLQLAERRRL
     NRMAERDVGD WLYARFLQPH AGTEQRFTAE IIDITRGGLR VRLADNGAVA FIPAPFLHAV
     RDELQCSQET GTVIIKGEVA YQLNDIIDVR IDEVRMETRN IVARPTA