ID   RNB_VIBC3               Reviewed;         678 AA.
AC   A5F0G1; C3M699;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Exoribonuclease 2 {ECO:0000255|HAMAP-Rule:MF_01036};
DE            EC=3.1.13.1 {ECO:0000255|HAMAP-Rule:MF_01036};
DE   AltName: Full=Exoribonuclease II {ECO:0000255|HAMAP-Rule:MF_01036};
DE            Short=RNase II {ECO:0000255|HAMAP-Rule:MF_01036};
DE            Short=Ribonuclease II {ECO:0000255|HAMAP-Rule:MF_01036};
GN   Name=rnb {ECO:0000255|HAMAP-Rule:MF_01036};
GN   OrderedLocusNames=VC0395_0429, VC395_A0829;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Involved in mRNA degradation. Hydrolyzes single-stranded
CC       polyribonucleotides processively in the 3' to 5' direction.
CC       {ECO:0000255|HAMAP-Rule:MF_01036}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01036};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01036}.
CC   -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase II subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01036}.
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DR   EMBL; CP000626; ABQ18712.1; -; Genomic_DNA.
DR   EMBL; CP001236; ACP11662.1; -; Genomic_DNA.
DR   RefSeq; WP_000485063.1; NZ_JAACZH010000012.1.
DR   AlphaFoldDB; A5F0G1; -.
DR   SMR; A5F0G1; -.
DR   STRING; 345073.VC395_A0829; -.
DR   EnsemblBacteria; ABQ18712; ABQ18712; VC0395_0429.
DR   KEGG; vco:VC0395_0429; -.
DR   KEGG; vcr:VC395_A0829; -.
DR   PATRIC; fig|345073.21.peg.3561; -.
DR   eggNOG; COG4776; Bacteria.
DR   HOGENOM; CLU_002333_7_3_6; -.
DR   OMA; CFTNYLP; -.
DR   Proteomes; UP000000249; Chromosome 1.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 2.
DR   HAMAP; MF_01036; RNase_II; 1.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR013223; RNase_B_OB_dom.
DR   InterPro; IPR011804; RNase_II.
DR   InterPro; IPR001900; RNase_II/R.
DR   InterPro; IPR022966; RNase_II/R_CS.
DR   InterPro; IPR004476; RNase_II/RNase_R.
DR   InterPro; IPR003029; S1_domain.
DR   Pfam; PF08206; OB_RNB; 1.
DR   Pfam; PF00773; RNB; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00357; CSP; 1.
DR   SMART; SM00955; RNB; 1.
DR   SUPFAM; SSF50249; SSF50249; 4.
DR   TIGRFAMs; TIGR00358; 3_prime_RNase; 1.
DR   TIGRFAMs; TIGR02062; RNase_B; 1.
DR   PROSITE; PS01175; RIBONUCLEASE_II; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; RNA-binding.
FT   CHAIN           1..678
FT                   /note="Exoribonuclease 2"
FT                   /id="PRO_1000072975"
FT   DOMAIN          568..650
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01036"
FT   REGION          658..678
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   678 AA;  76155 MW;  EA026FF6993F1808 CRC64;
     MFQDNPRLAQ LKQKIQETLP KKEGTIKASD KGFGFLEVDS KTSYFVPPPY MKKCMHGDKV
     VAFIRTENER EVAEPSELIE QSLTRFIGRV KLFKGKLNVA PDHPQLKKLS LKAKTKKGLN
     EADFQEGDWV VAHLVRHPLK GDDGFFVQIS HKITDANDKI APWWVTLAEN DLPNSEPAGI
     DDWQLKDDAD LVREDLTALP FVTIDGESTK DMDDALYAQQ LPNGDFALTI AIADPTAYIT
     PEDEMDKVAR ERGFTIYLPG RNIPMLPRDL ADELCSLMEN QVRPALCCSV TIRKDGVIGD
     DIRFFAANIK SHARLVYDHV SDWLETGSSE QWQPSEEIAQ VVRDLYAFSQ ARANWCETHA
     VVFPDRPDYR FELSADNDVV AIHADMRRTA NRLVEESMIT ANICAGKTLQ TTFGFGVFNT
     HAGFKAEKMA DVVELMAVNG APNADAETLA TVEGFAALRR WLATQETSYL DNRIRKYQSY
     SEIGNQPLPH FAMGLDVYAT WTSPIRKYGD MINHRLLKAH ILGKAPVQTP DETVGEELAL
     HRKHHKIAER NVADWLYART LADEPAKETR FQAEIFDINR PGMRVRLLEN GAMAFIPGAL
     ILDNKERIEC NGEDGTILID KEVVYKLGDV LEIVLTEVNQ ENRSLVGKPT QVFADLVSET
     QTSTEQPAEG AENNEPQA