RNC_MYCS2
ID RNC_MYCS2 Reviewed; 230 AA.
AC A0QV20;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Ribonuclease III;
DE Short=RNase III;
GN Name=rnc; OrderedLocusNames=MSMEG_2418, MSMEI_2357;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22014150; DOI=10.1111/j.1365-2958.2011.07888.x;
RA Taverniti V., Forti F., Ghisotti D., Putzer H.;
RT "Mycobacterium smegmatis RNase J is a 5'-3' exo-/endoribonuclease and both
RT RNase J and RNase E are involved in ribosomal RNA maturation.";
RL Mol. Microbiol. 82:1260-1276(2011).
CC -!- FUNCTION: Digests double-stranded RNA. Involved in the processing of
CC primary rRNA transcript to yield the immediate precursors to the all
CC rRNAs (23S, 16S and 5S) (Probable). Processes some mRNAs, and tRNAs
CC when they are encoded in the rRNA operon. Processes pre-crRNA and
CC tracrRNA of type II CRISPR loci if present in the organism (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:22014150, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ribonuclease III family. {ECO:0000305}.
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DR EMBL; CP000480; ABK72959.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP38825.1; -; Genomic_DNA.
DR RefSeq; WP_011728329.1; NZ_SIJM01000012.1.
DR RefSeq; YP_886758.1; NC_008596.1.
DR AlphaFoldDB; A0QV20; -.
DR SMR; A0QV20; -.
DR STRING; 246196.MSMEI_2357; -.
DR EnsemblBacteria; ABK72959; ABK72959; MSMEG_2418.
DR EnsemblBacteria; AFP38825; AFP38825; MSMEI_2357.
DR GeneID; 66733832; -.
DR KEGG; msg:MSMEI_2357; -.
DR KEGG; msm:MSMEG_2418; -.
DR PATRIC; fig|246196.19.peg.2383; -.
DR eggNOG; COG0571; Bacteria.
DR OMA; LTHKSCK; -.
DR OrthoDB; 1890943at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-UniRule.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd00593; RIBOc; 1.
DR Gene3D; 1.10.1520.10; -; 1.
DR HAMAP; MF_00104; RNase_III; 1.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR11207; PTHR11207; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF14622; Ribonucleas_3_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; SSF69065; 1.
DR TIGRFAMs; TIGR02191; RNaseIII; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW mRNA processing; Nuclease; Reference proteome; RNA-binding;
KW rRNA processing; rRNA-binding; tRNA processing.
FT CHAIN 1..230
FT /note="Ribonuclease 3"
FT /id="PRO_0000429580"
FT DOMAIN 19..134
FT /note="RNase III"
FT DOMAIN 161..229
FT /note="DRBM"
FT ACT_SITE 48
FT /evidence="ECO:0000250"
FT BINDING 44
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 120
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 123
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
SQ SEQUENCE 230 AA; 24205 MW; 4AAF4F9CCCFC5BFC CRC64;
MTDSHAALLE ALGVALPAEL LTIALTHRSY SYENGGLPTN ERLEFLGDAV LGLTITEELY
HRHPDRAEGD LAKLRASIVN TQALADVGRG LTDEGLGAHL FLGKGEENSG GADKSSILAD
GVESLLGAIY LEHGLTVVRE VILRLFGELL DTAPTLGAGL DWKSSLQELT AARGLGAPAY
VVTSTGPDHD KEFSATVVIG EAEYGHGVGR TKKEAELKAA ASAYKTLDES
