ID   RNC_TRES6               Reviewed;         369 AA.
AC   F2NV13;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Ribonuclease 3;
DE            EC=3.1.26.3;
DE   AltName: Full=Ribonuclease III;
DE            Short=RNase III;
GN   Name=rnc; OrderedLocusNames=Tresu_0151;
OS   Treponema succinifaciens (strain ATCC 33096 / DSM 2489 / 6091).
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=869209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33096 / DSM 2489 / 6091;
RA   Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA   Peters L., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G.,
RA   Teshima H., Detter J.C., Tapia R., Han C., Land M., Hauser L.,
RA   Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Gronow S.,
RA   Wellnitz S., Brambilla E., Klenk H.-P., Eisen J.A.;
RT   "The complete genome of chromosome of Treponema succinifaciens DSM 2489.";
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Digests double-stranded RNA. Involved in the processing of
CC       primary rRNA transcript to yield the immediate precursors to the large
CC       and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when
CC       they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA
CC       of type II CRISPR loci if present in the organism (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ribonuclease III family. {ECO:0000305}.
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DR   EMBL; CP002631; AEB13117.1; -; Genomic_DNA.
DR   RefSeq; WP_013700428.1; NC_015385.1.
DR   AlphaFoldDB; F2NV13; -.
DR   SMR; F2NV13; -.
DR   STRING; 869209.Tresu_0151; -.
DR   EnsemblBacteria; AEB13117; AEB13117; Tresu_0151.
DR   KEGG; tsu:Tresu_0151; -.
DR   eggNOG; COG0571; Bacteria.
DR   HOGENOM; CLU_775860_0_0_12; -.
DR   OrthoDB; 1890943at2; -.
DR   Proteomes; UP000006852; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd00593; RIBOc; 1.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   HAMAP; MF_00104; RNase_III; 1.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR011907; RNase_III.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00035; dsrm; 1.
DR   Pfam; PF14622; Ribonucleas_3_3; 1.
DR   SMART; SM00535; RIBOc; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
DR   PROSITE; PS50137; DS_RBD; 1.
DR   PROSITE; PS50142; RNASE_3_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   mRNA processing; Nuclease; Reference proteome; RNA-binding;
KW   rRNA processing; rRNA-binding; tRNA processing.
FT   CHAIN           1..369
FT                   /note="Ribonuclease 3"
FT                   /id="PRO_0000416614"
FT   DOMAIN          6..142
FT                   /note="RNase III"
FT   DOMAIN          272..341
FT                   /note="DRBM"
FT   ACT_SITE        50
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        131
FT                   /evidence="ECO:0000250"
FT   BINDING         46
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         128
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         131
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   369 AA;  42826 MW;  6657D14A15ED1B13 CRC64;
     MNRQDIGFVQ SSINYQFKNL QLLEQAFTRK SYSEEHPELQ NNEVLEFYGD EILDFFVTKM
     MYKRFSRILN NELVSEKNEG ELTKLKSILV SKESLARCMY NIGFSKFLYL GKSDEKNEVY
     KSKSVNEDLF EAIIGAVAAD CDWNYEKLEK VCRTMLDMET TNGYLALLVK EKSHRLGFGE
     PCYRPFAWQT DNPEDWQSDN LFNMGIGFGG FGSTSKNPKT GKHEYGIQVG ENKFTGIGDG
     IAQAKLNAEQ KAYHFLIQEE IKQQFQNLDY TNPASTLHEL FQKKVIMEVR YEFNEYHDEN
     GNPIWNCKAI LEGYGTFEAD NASKKQVKQD ASLKLLKFIA KTKIEQTEKW EIPTFYHGMA
     RIWHDEGKI