RNF32_HUMAN
ID RNF32_HUMAN Reviewed; 362 AA.
AC Q9H0A6; Q6FIB3; Q6X7T4; Q8N6V8; Q8TDG0; Q96BM5; Q9Y6U1;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=RING finger protein 32;
GN Name=RNF32; ORFNames=FKSG33, HSD15;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND ALTERNATIVE SPLICING.
RX PubMed=11890671; DOI=10.1006/bbrc.2002.6612;
RA van Baren M.J., van der Linde H.C., Breedveld G.J., Baarends W.M.,
RA Rizzu P., de Graaff E., Oostra B.A., Heutink P.;
RT "A double RING-H2 domain in RNF32, a gene expressed during sperm
RT formation.";
RL Biochem. Biophys. Res. Commun. 292:58-65(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Wang Y.-G., Gong L.;
RT "Characterization of FKSG33, a novel gene located on human chromosome 7q35-
RT 36.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Testis;
RA Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.;
RT "A new spermatogenesis-related gene.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Duodenum;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in sperm formation.
CC {ECO:0000269|PubMed:11890671}.
CC -!- INTERACTION:
CC Q9H0A6; Q9NXK8: FBXL12; NbExp=2; IntAct=EBI-724829, EBI-719790;
CC Q9H0A6; Q96QH2: PRAM1; NbExp=2; IntAct=EBI-724829, EBI-2860740;
CC Q9H0A6; Q96FJ0: STAMBPL1; NbExp=2; IntAct=EBI-724829, EBI-745021;
CC Q9H0A6-4; P61086: UBE2K; NbExp=3; IntAct=EBI-25868153, EBI-473850;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11890671}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Comment=Other isoforms seem to exist.;
CC Name=1;
CC IsoId=Q9H0A6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H0A6-2; Sequence=VSP_019740;
CC Name=3;
CC IsoId=Q9H0A6-4; Sequence=VSP_019743, VSP_019744;
CC Name=4;
CC IsoId=Q9H0A6-5; Sequence=VSP_019741;
CC Name=5;
CC IsoId=Q9H0A6-6; Sequence=VSP_019738, VSP_019739;
CC -!- TISSUE SPECIFICITY: Highly expressed in testis, less abundant in ovary.
CC {ECO:0000269|PubMed:11890671}.
CC -!- MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
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DR EMBL; AF441222; AAM18664.1; -; mRNA.
DR EMBL; AF441224; AAM18666.1; -; mRNA.
DR EMBL; AF325690; AAG50281.1; -; mRNA.
DR EMBL; AL136874; CAB66808.1; -; mRNA.
DR EMBL; AY251165; AAP20062.1; -; mRNA.
DR EMBL; BT007037; AAP35686.1; -; mRNA.
DR EMBL; CR533513; CAG38544.1; -; mRNA.
DR EMBL; AC005534; AAD43189.1; -; Genomic_DNA.
DR EMBL; BC015416; AAH15416.1; -; mRNA.
DR CCDS; CCDS5944.1; -. [Q9H0A6-1]
DR CCDS; CCDS78292.1; -. [Q9H0A6-4]
DR RefSeq; NP_001171925.1; NM_001184996.1. [Q9H0A6-1]
DR RefSeq; NP_001171926.1; NM_001184997.1. [Q9H0A6-1]
DR RefSeq; NP_001295202.1; NM_001308273.1.
DR RefSeq; NP_001295203.1; NM_001308274.1. [Q9H0A6-4]
DR RefSeq; NP_112198.1; NM_030936.3. [Q9H0A6-1]
DR RefSeq; XP_005249579.1; XM_005249522.4. [Q9H0A6-1]
DR RefSeq; XP_011514106.1; XM_011515804.2. [Q9H0A6-1]
DR RefSeq; XP_011514107.1; XM_011515805.2. [Q9H0A6-1]
DR RefSeq; XP_011514108.1; XM_011515806.2. [Q9H0A6-1]
DR RefSeq; XP_011514109.1; XM_011515807.2. [Q9H0A6-1]
DR RefSeq; XP_011514110.1; XM_011515808.2. [Q9H0A6-1]
DR RefSeq; XP_011514111.1; XM_011515809.2. [Q9H0A6-1]
DR RefSeq; XP_011514112.1; XM_011515810.2. [Q9H0A6-1]
DR RefSeq; XP_011514113.1; XM_011515811.2. [Q9H0A6-1]
DR RefSeq; XP_011514114.1; XM_011515812.2. [Q9H0A6-1]
DR RefSeq; XP_016867244.1; XM_017011755.1. [Q9H0A6-4]
DR AlphaFoldDB; Q9H0A6; -.
DR SMR; Q9H0A6; -.
DR BioGRID; 126622; 46.
DR IntAct; Q9H0A6; 33.
DR STRING; 9606.ENSP00000385285; -.
DR MoonDB; Q9H0A6; Predicted.
DR PhosphoSitePlus; Q9H0A6; -.
DR BioMuta; RNF32; -.
DR DMDM; 74717970; -.
DR jPOST; Q9H0A6; -.
DR MassIVE; Q9H0A6; -.
DR PaxDb; Q9H0A6; -.
DR PeptideAtlas; Q9H0A6; -.
DR PRIDE; Q9H0A6; -.
DR Antibodypedia; 18881; 80 antibodies from 21 providers.
DR DNASU; 140545; -.
DR Ensembl; ENST00000317955.10; ENSP00000315950.5; ENSG00000105982.17. [Q9H0A6-1]
DR Ensembl; ENST00000392741.6; ENSP00000376497.2; ENSG00000105982.17. [Q9H0A6-4]
DR Ensembl; ENST00000392743.6; ENSP00000376499.2; ENSG00000105982.17. [Q9H0A6-1]
DR Ensembl; ENST00000392747.6; ENSP00000376503.2; ENSG00000105982.17. [Q9H0A6-6]
DR Ensembl; ENST00000405335.5; ENSP00000385285.1; ENSG00000105982.17. [Q9H0A6-1]
DR Ensembl; ENST00000432459.6; ENSP00000405588.2; ENSG00000105982.17. [Q9H0A6-1]
DR GeneID; 140545; -.
DR KEGG; hsa:140545; -.
DR MANE-Select; ENST00000317955.10; ENSP00000315950.5; NM_030936.4; NP_112198.1.
DR UCSC; uc003wmo.4; human. [Q9H0A6-1]
DR CTD; 140545; -.
DR DisGeNET; 140545; -.
DR GeneCards; RNF32; -.
DR HGNC; HGNC:17118; RNF32.
DR HPA; ENSG00000105982; Tissue enriched (testis).
DR MIM; 610241; gene.
DR neXtProt; NX_Q9H0A6; -.
DR OpenTargets; ENSG00000105982; -.
DR PharmGKB; PA34435; -.
DR VEuPathDB; HostDB:ENSG00000105982; -.
DR eggNOG; KOG0800; Eukaryota.
DR GeneTree; ENSGT00390000003759; -.
DR HOGENOM; CLU_064517_0_0_1; -.
DR InParanoid; Q9H0A6; -.
DR OMA; KWYKNLR; -.
DR PhylomeDB; Q9H0A6; -.
DR TreeFam; TF329796; -.
DR PathwayCommons; Q9H0A6; -.
DR SignaLink; Q9H0A6; -.
DR BioGRID-ORCS; 140545; 13 hits in 1116 CRISPR screens.
DR ChiTaRS; RNF32; human.
DR GeneWiki; RNF32; -.
DR GenomeRNAi; 140545; -.
DR Pharos; Q9H0A6; Tdark.
DR PRO; PR:Q9H0A6; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9H0A6; protein.
DR Bgee; ENSG00000105982; Expressed in left testis and 108 other tissues.
DR ExpressionAtlas; Q9H0A6; baseline and differential.
DR Genevisible; Q9H0A6; HS.
DR GO; GO:0016235; C:aggresome; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005768; C:endosome; IDA:LIFEdb.
DR GO; GO:0016604; C:nuclear body; IDA:HPA.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.40.10; -; 2.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR042862; RNF32.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR14991; PTHR14991; 1.
DR Pfam; PF00612; IQ; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00184; RING; 2.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS50089; ZF_RING_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Metal-binding; Reference proteome; Repeat;
KW Zinc; Zinc-finger.
FT CHAIN 1..362
FT /note="RING finger protein 32"
FT /id="PRO_0000245532"
FT DOMAIN 186..215
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT ZN_FING 127..169
FT /note="RING-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 293..352
FT /note="RING-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT VAR_SEQ 92..95
FT /note="AQKL -> GYHS (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11890671"
FT /id="VSP_019738"
FT VAR_SEQ 96..362
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11890671"
FT /id="VSP_019739"
FT VAR_SEQ 141..169
FT /note="LLSCSHVFHKACLQAFEKFTNKKTCPLCR -> FSIRG (in isoform
FT 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_019740"
FT VAR_SEQ 170..352
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_019741"
FT VAR_SEQ 229..235
FT /note="FTEISHR -> TQDWKPA (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.5"
FT /id="VSP_019743"
FT VAR_SEQ 236..362
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.5"
FT /id="VSP_019744"
FT VARIANT 288
FT /note="R -> Q (in dbSNP:rs2302148)"
FT /id="VAR_026982"
FT VARIANT 291
FT /note="H -> Q (in dbSNP:rs2302147)"
FT /id="VAR_026983"
FT VARIANT 307
FT /note="R -> C (in dbSNP:rs2302146)"
FT /id="VAR_026984"
FT CONFLICT 97
FT /note="L -> P (in Ref. 6; CAG38544)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 362 AA; 41516 MW; 5C6AF6908FCCA456 CRC64;
MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII
DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ
GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRK NQYQTRVIHD
GARLFRIKCV TRIQAYWRGC VVRKWYRNLR KTVPPTDAKL RKKFFEKKFT EISHRILCSY
NTNIEELFAE IDQCLAINRS VLQQLEEKCG HEITEEEWEK IQVQALRRET HECSICLAPL
SAAGGQRVGA GRRSREMALL SCSHVFHHAC LLALEEFSVG DRPPFHACPL CRSCYQKKIL
EC
