RNF32_MOUSE
ID RNF32_MOUSE Reviewed; 368 AA.
AC Q9JIT1; Q9D9X0; Q9DAJ3;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=RING finger protein 32;
DE AltName: Full=Limb region protein 2;
GN Name=Rnf32; Synonyms=Lmbr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/10;
RX PubMed=10945466; DOI=10.1006/geno.2000.6225;
RA Clark R.M., Marker P.C., Kingsley D.M.;
RT "A novel candidate gene for mouse and human preaxial polydactyly with
RT altered expression in limbs of hemimelic extra-toes mutant mice.";
RL Genomics 67:19-27(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in sperm formation.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- MISCELLANEOUS: No coding region alterations in either Hemimelic extra-
CC toes (Hx) or Hammertoe (Hm) mutant mice was detected.
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DR EMBL; AF190664; AAF91091.1; -; mRNA.
DR EMBL; AK005795; BAB24242.1; -; mRNA.
DR EMBL; AK006379; BAB24557.1; -; mRNA.
DR EMBL; BC089493; AAH89493.1; -; mRNA.
DR CCDS; CCDS39041.1; -.
DR RefSeq; NP_001276686.1; NM_001289757.1.
DR RefSeq; NP_067445.3; NM_021470.6.
DR RefSeq; XP_006535838.1; XM_006535775.3.
DR RefSeq; XP_006535839.1; XM_006535776.3.
DR RefSeq; XP_006535840.1; XM_006535777.3.
DR RefSeq; XP_006535841.1; XM_006535778.3.
DR RefSeq; XP_006535842.1; XM_006535779.3.
DR RefSeq; XP_006535843.1; XM_006535780.2.
DR RefSeq; XP_006535844.1; XM_006535781.3.
DR AlphaFoldDB; Q9JIT1; -.
DR SMR; Q9JIT1; -.
DR STRING; 10090.ENSMUSP00000001247; -.
DR iPTMnet; Q9JIT1; -.
DR PhosphoSitePlus; Q9JIT1; -.
DR PaxDb; Q9JIT1; -.
DR PRIDE; Q9JIT1; -.
DR ProteomicsDB; 260988; -.
DR Antibodypedia; 18881; 80 antibodies from 21 providers.
DR DNASU; 56874; -.
DR Ensembl; ENSMUST00000001247; ENSMUSP00000001247; ENSMUSG00000029130.
DR Ensembl; ENSMUST00000160246; ENSMUSP00000124657; ENSMUSG00000029130.
DR Ensembl; ENSMUST00000168460; ENSMUSP00000132213; ENSMUSG00000029130.
DR GeneID; 56874; -.
DR KEGG; mmu:56874; -.
DR UCSC; uc008wuc.2; mouse.
DR CTD; 140545; -.
DR MGI; MGI:1861747; Rnf32.
DR VEuPathDB; HostDB:ENSMUSG00000029130; -.
DR eggNOG; KOG0800; Eukaryota.
DR GeneTree; ENSGT00390000003759; -.
DR InParanoid; Q9JIT1; -.
DR OMA; KWYKNLR; -.
DR OrthoDB; 1189760at2759; -.
DR PhylomeDB; Q9JIT1; -.
DR TreeFam; TF329796; -.
DR BioGRID-ORCS; 56874; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Rnf32; mouse.
DR PRO; PR:Q9JIT1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9JIT1; protein.
DR Bgee; ENSMUSG00000029130; Expressed in spermatocyte and 182 other tissues.
DR ExpressionAtlas; Q9JIT1; baseline and differential.
DR Genevisible; Q9JIT1; MM.
DR GO; GO:0016235; C:aggresome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005768; C:endosome; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.40.10; -; 2.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR042862; RNF32.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR14991; PTHR14991; 1.
DR Pfam; PF00612; IQ; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00184; RING; 2.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS50089; ZF_RING_2; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..368
FT /note="RING finger protein 32"
FT /id="PRO_0000245534"
FT DOMAIN 188..217
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT ZN_FING 129..171
FT /note="RING-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 295..358
FT /note="RING-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 45..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 84
FT /note="L -> I (in Ref. 2; BAB24557)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="L -> I (in Ref. 2; BAB24557)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="E -> G (in Ref. 2; BAB24242)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="G -> R (in Ref. 2; BAB24242)"
FT /evidence="ECO:0000305"
FT CONFLICT 245
FT /note="N -> K (in Ref. 2; BAB24557)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 42139 MW; 23F8DE23EF4D02AC CRC64;
MLRNKDRSSN KGNLAVTAVA LQDHLLHDLQ LGHLSVADPC KIKARKKEKK SKSLKRDATA
IIDTGLRKST EGPNMEDPEK EYVLDPTPPP LTLAQKLGLL PPPPLPLSSD EWERVKQRSL
LQGDSIQPCP ICKEEFELHP QVLLSCSHVF HRACLQAFEK FTNKKTCPLC RKNQYQTRVI
HDGARLFRVK CATRIQAYWR GYIVRKWYRN LRKIIPPSDA KLRRKFFEEK FTEISHRILC
SYNTNIDELF SEIDVCLAVN RSILQQLDER CGQIITKEDW EKIQAQAAHH EIYECSICLT
PLSFHGDGRQ AAIGTSSQRP RETVLLSCAH LFHNACLLAL EEFSLGDNAP FHVCPLCRSC
YQKKIVEC
