RNFA_ACEWD
ID RNFA_ACEWD Reviewed; 192 AA.
AC H6LC28; C4N8U4;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 2.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Na(+)-translocating ferredoxin:NAD(+) oxidoreductase complex subunit A {ECO:0000305};
DE EC=7.2.1.2 {ECO:0000269|PubMed:20921383, ECO:0000269|PubMed:24045950};
DE AltName: Full=Rnf electron transport complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
GN Name=rnfA {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000303|PubMed:19222539};
GN OrderedLocusNames=Awo_c22020 {ECO:0000312|EMBL:AFA48976.1};
OS Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655
OS / WB1).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
OC Acetobacterium.
OX NCBI_TaxID=931626;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX PubMed=19222539; DOI=10.1111/j.1462-2920.2009.01871.x;
RA Biegel E., Schmidt S., Muller V.;
RT "Genetic, immunological and biochemical evidence for a Rnf complex in the
RT acetogen Acetobacterium woodii.";
RL Environ. Microbiol. 11:1438-1443(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RA Poehlein A., Schmidt S., Kaster A.-K., Goenrich M., Vollmers J.,
RA Thuermer A., Gottschalk G., Thauer R.K., Daniel R., Mueller V.;
RT "Complete genome sequence of Acetobacterium woodii.";
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBUNIT.
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX PubMed=20921383; DOI=10.1073/pnas.1010318107;
RA Biegel E., Mueller V.;
RT "Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18138-18142(2010).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX PubMed=24045950; DOI=10.1074/jbc.m113.510255;
RA Hess V., Schuchmann K., Mueller V.;
RT "The ferredoxin:NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium
RT woodii requires Na+ and is reversibly coupled to the membrane potential.";
RL J. Biol. Chem. 288:31496-31502(2013).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane. Couples
CC electron transfer from reduced ferredoxin to NAD(+) with electrogenic
CC movement of Na(+) out of the cell. Involved in caffeate respiration.
CC {ECO:0000269|PubMed:20921383, ECO:0000269|PubMed:24045950}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + Na(+)(in) + NAD(+) + 2 reduced [2Fe-2S]-[ferredoxin] =
CC Na(+)(out) + NADH + 2 oxidized [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:46800, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33737,
CC ChEBI:CHEBI:33738, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.2.1.2;
CC Evidence={ECO:0000269|PubMed:20921383, ECO:0000269|PubMed:24045950};
CC -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00459,
CC ECO:0000305|PubMed:20921383}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00459};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00459}.
CC -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC Rule:MF_00459}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFA48976.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FJ416148; ACR23746.1; -; Genomic_DNA.
DR EMBL; CP002987; AFA48976.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_041668780.1; NC_016894.1.
DR AlphaFoldDB; H6LC28; -.
DR SMR; H6LC28; -.
DR STRING; 931626.Awo_c22020; -.
DR TCDB; 3.D.6.1.2; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR EnsemblBacteria; AFA48976; AFA48976; Awo_c22020.
DR KEGG; awo:Awo_c22020; -.
DR eggNOG; COG4657; Bacteria.
DR HOGENOM; CLU_095255_1_0_9; -.
DR OrthoDB; 1814639at2; -.
DR BioCyc; MetaCyc:MON-21339; -.
DR BRENDA; 7.2.1.2; 52.
DR Proteomes; UP000007177; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00459; RsxA_RnfA; 1.
DR InterPro; IPR011293; Ion_transpt_RnfA/RsxA.
DR InterPro; IPR003667; NqrDE/RnfAE.
DR Pfam; PF02508; Rnf-Nqr; 1.
DR PIRSF; PIRSF006102; NQR_DE; 1.
DR TIGRFAMs; TIGR01943; rnfA; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Electron transport; Membrane; NAD; Reference proteome;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..192
FT /note="Na(+)-translocating ferredoxin:NAD(+) oxidoreductase
FT complex subunit A"
FT /id="PRO_0000443482"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT CONFLICT 107
FT /note="P -> PFLP (in Ref. 1; ACR23746)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 192 AA; 20434 MW; 9037923D6D274449 CRC64;
MTLIFIMISA IFVNNFVLSR FLGICPFLGV SKQVETAVGM GVAVTFVMAL ASAITYVVQY
AILDPLSLGY LQTIAFILII AALVQLVEMI IKKSSPSLYQ ALGVYLPLIT TNCAVLGVAL
INIQNEYNFI ETIFNGVGAA LGFTLAIVLF AGIRERLETS AVPKALEGFP IALLTAGLMA
IAFLGFSGMK LG
