RNFA_METAC
ID RNFA_METAC Reviewed; 199 AA.
AC Q8TSY0;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Ion-translocating oxidoreductase complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE EC=7.2.1.- {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE AltName: Full=Rnf electron transport complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
GN Name=rnfA {ECO:0000255|HAMAP-Rule:MF_00459};
GN OrderedLocusNames=MA_0663 {ECO:0000312|EMBL:AAM04105.1};
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
RN [2]
RP FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=23066798; DOI=10.1111/febs.12031;
RA Schlegel K., Welte C., Deppenmeier U., Mueller V.;
RT "Electron transport during aceticlastic methanogenesis by Methanosarcina
RT acetivorans involves a sodium-translocating Rnf complex.";
RL FEBS J. 279:4444-4452(2012).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane. Catalyzes
CC Na(+) transport, most probably coupled to electron transfer from
CC reduced ferredoxin to methanophenazine and heterodisulfide reductase.
CC Involved in heterodisulfide reduction during methanogenesis from
CC acetate. {ECO:0000269|PubMed:23066798}.
CC -!- SUBUNIT: The Rnf complex is probably composed of eight subunits,
CC including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-
CC Rule:MF_00459, ECO:0000305|PubMed:23066798}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00459};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00459}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the rnf operon abolishes growth on
CC acetate and ferredoxin:heterodisulfide oxidoreductase-coupled Na(+)
CC transport. {ECO:0000269|PubMed:23066798}.
CC -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC Rule:MF_00459}.
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DR EMBL; AE010299; AAM04105.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8TSY0; -.
DR SMR; Q8TSY0; -.
DR STRING; 188937.MA_0663; -.
DR TCDB; 3.D.6.1.3; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR EnsemblBacteria; AAM04105; AAM04105; MA_0663.
DR KEGG; mac:MA_0663; -.
DR HOGENOM; CLU_095255_1_0_2; -.
DR InParanoid; Q8TSY0; -.
DR OMA; CPFFGVS; -.
DR PhylomeDB; Q8TSY0; -.
DR BRENDA; 7.2.1.2; 7224.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00459; RsxA_RnfA; 1.
DR InterPro; IPR011293; Ion_transpt_RnfA/RsxA.
DR InterPro; IPR003667; NqrDE/RnfAE.
DR Pfam; PF02508; Rnf-Nqr; 1.
DR PIRSF; PIRSF006102; NQR_DE; 1.
DR TIGRFAMs; TIGR01943; rnfA; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Electron transport; Membrane; Reference proteome;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..199
FT /note="Ion-translocating oxidoreductase complex subunit A"
FT /id="PRO_0000443485"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
SQ SEQUENCE 199 AA; 21677 MW; AC1DBDF88FBC5113 CRC64;
MVKMAESLFT IFLEGVFIKN FLLIQFLGLC SFVGVTKDLK SASGMSGAVV FVMAMAATVS
FALYNFILVP LKLEFLRTIA FIVVIAALVQ LVEFIVRKHV PALYRSLGIY LPLITTNCAV
LGAVLLNVMN DYDLAQSVVF GVAAGLGYTV AMLMMAAIRE RSDLVEVPKS VGRGVTYAFF
IATIMSMSFV NFFGVIPLE
