ID   RNFA_RHOCA              Reviewed;         193 AA.
AC   P0CZ13; Q07396;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Ion-translocating oxidoreductase complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE   AltName: Full=Nitrogen fixation protein RnfA {ECO:0000305};
DE   AltName: Full=Rnf electron transport complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
GN   Name=rnfA {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000303|PubMed:8264535};
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GENE NAME.
RC   STRAIN=B10S;
RX   PubMed=8264535; DOI=10.1007/bf00279903;
RA   Schmehl M., Jahn A., Meyer zu Vilsendorf A., Hennecke S., Masepohl B.,
RA   Schuppler M., Marxer M., Oelze J., Klipp W.;
RT   "Identification of a new class of nitrogen fixation genes in Rhodobacter
RT   capsulatus: a putative membrane complex involved in electron transport to
RT   nitrogenase.";
RL   Mol. Gen. Genet. 241:602-615(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, AND INDUCTION.
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=9492268; DOI=10.1046/j.1432-1327.1998.2510054.x;
RA   Jouanneau Y., Jeong H.-S., Hugo N., Meyer C., Willison J.C.;
RT   "Overexpression in Escherichia coli of the rnf genes from Rhodobacter
RT   capsulatus -- characterization of two membrane-bound iron-sulfur
RT   proteins.";
RL   Eur. J. Biochem. 251:54-64(1998).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane (By
CC       similarity). Required for nitrogen fixation. Involved in electron
CC       transfer to nitrogenase (PubMed:8264535). {ECO:0000255|HAMAP-
CC       Rule:MF_00459, ECO:0000269|PubMed:8264535}.
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC       RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00459,
CC       ECO:0000305|PubMed:9492268}.
CC   -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane
CC       {ECO:0000250|UniProtKB:D5ARY9, ECO:0000255|HAMAP-Rule:MF_00459}; Multi-
CC       pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00459}.
CC   -!- INDUCTION: Expression is reduced under iron-limiting conditions.
CC       {ECO:0000269|PubMed:9492268}.
CC   -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00459}.
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DR   EMBL; X72888; CAA51401.1; -; Genomic_DNA.
DR   EMBL; Y11913; CAA72668.1; -; Genomic_DNA.
DR   PIR; S39895; S39895.
DR   RefSeq; WP_013068983.1; NZ_VIBE01000016.1.
DR   AlphaFoldDB; P0CZ13; -.
DR   SMR; P0CZ13; -.
DR   GeneID; 31492067; -.
DR   OMA; CPFFGVS; -.
DR   GO; GO:0016021; C:integral component of membrane; IDA:CACAO.
DR   GO; GO:0070111; C:organellar chromatophore; IDA:CACAO.
DR   GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00459; RsxA_RnfA; 1.
DR   InterPro; IPR011293; Ion_transpt_RnfA/RsxA.
DR   InterPro; IPR003667; NqrDE/RnfAE.
DR   Pfam; PF02508; Rnf-Nqr; 1.
DR   PIRSF; PIRSF006102; NQR_DE; 1.
DR   TIGRFAMs; TIGR01943; rnfA; 1.
PE   1: Evidence at protein level;
KW   Electron transport; Membrane; Nitrogen fixation; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..193
FT                   /note="Ion-translocating oxidoreductase complex subunit A"
FT                   /id="PRO_0000214296"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
SQ   SEQUENCE   193 AA;  20424 MW;  2C4FE33A66290C3D CRC64;
     MQDFLLVLLS TALVNNVVLV KFLGLCPFMG VSRKTDAAIG MGLATTFVIT VASAACWLVE
     ALILEPLDLK FLRILSMILV IAAIVQFIET VMRKVTPDLH KALGIYLPLI TTNCAVLGLP
     LMYIQGHLSL AMSTLSGFGA SVGFTLVLVI FAGMRERLAQ LSVPAAFAGT PIAFVSAGLL
     GLAFMGFAGL VHV