位置:首页 > 蛋白库 > RNFB_RHOCA
RNFB_RHOCA
ID   RNFB_RHOCA              Reviewed;         187 AA.
AC   P0CZ14; O08056; Q07394;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Ion-translocating oxidoreductase complex subunit B {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000305};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000305};
DE   AltName: Full=Nitrogen fixation protein RnfB {ECO:0000305};
DE   AltName: Full=Rnf electron transport complex subunit B {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000305};
GN   Name=rnfB {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000303|PubMed:8264535};
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GENE NAME.
RC   STRAIN=B10S;
RX   PubMed=8264535; DOI=10.1007/bf00279903;
RA   Schmehl M., Jahn A., Meyer zu Vilsendorf A., Hennecke S., Masepohl B.,
RA   Schuppler M., Marxer M., Oelze J., Klipp W.;
RT   "Identification of a new class of nitrogen fixation genes in Rhodobacter
RT   capsulatus: a putative membrane complex involved in electron transport to
RT   nitrogenase.";
RL   Mol. Gen. Genet. 241:602-615(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, SUBUNIT,
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=9492268; DOI=10.1046/j.1432-1327.1998.2510054.x;
RA   Jouanneau Y., Jeong H.-S., Hugo N., Meyer C., Willison J.C.;
RT   "Overexpression in Escherichia coli of the rnf genes from Rhodobacter
RT   capsulatus -- characterization of two membrane-bound iron-sulfur
RT   proteins.";
RL   Eur. J. Biochem. 251:54-64(1998).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane (By
CC       similarity). Required for nitrogen fixation. Involved in electron
CC       transfer to nitrogenase (PubMed:8264535). {ECO:0000255|HAMAP-
CC       Rule:MF_00463, ECO:0000269|PubMed:8264535}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00463};
CC       Note=Binds 3 [4Fe-4S] clusters. {ECO:0000255|HAMAP-Rule:MF_00463};
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC       RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00463,
CC       ECO:0000305|PubMed:9492268}.
CC   -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane
CC       {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000269|PubMed:9492268};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00463,
CC       ECO:0000269|PubMed:9492268}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:D5ARZ0, ECO:0000255|HAMAP-Rule:MF_00463}.
CC   -!- INDUCTION: Expression is reduced under iron-limiting conditions.
CC       {ECO:0000269|PubMed:9492268}.
CC   -!- SIMILARITY: Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00463}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X72888; CAA51400.1; -; Genomic_DNA.
DR   EMBL; Y11913; CAA72669.1; -; Genomic_DNA.
DR   PIR; S39894; S39894.
DR   RefSeq; WP_013068984.1; NZ_VIBE01000016.1.
DR   AlphaFoldDB; P0CZ14; -.
DR   GeneID; 31492068; -.
DR   OMA; DEENCIG; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00463; RsxB_RnfB; 1.
DR   InterPro; IPR007202; 4Fe-4S_dom.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR010207; Elect_transpt_cplx_RnfB/RsxB.
DR   InterPro; IPR016463; RnfB/RsxB_Proteobac.
DR   PANTHER; PTHR42859:SF3; PTHR42859:SF3; 1.
DR   Pfam; PF04060; FeS; 1.
DR   PIRSF; PIRSF005784; Elect_transpt_RnfB; 1.
DR   TIGRFAMs; TIGR01944; rnfB; 1.
DR   PROSITE; PS51656; 4FE4S; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur;
KW   Membrane; Metal-binding; Nitrogen fixation; Repeat; Translocase; Transport.
FT   CHAIN           1..187
FT                   /note="Ion-translocating oxidoreductase complex subunit B"
FT                   /id="PRO_0000216277"
FT   DOMAIN          29..88
FT                   /note="4Fe-4S"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   DOMAIN          103..132
FT                   /note="4Fe-4S ferredoxin-type 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   DOMAIN          133..162
FT                   /note="4Fe-4S ferredoxin-type 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   REGION          1..23
FT                   /note="Hydrophobic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         46
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         49
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         54
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         71
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         112
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         115
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         118
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         122
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         142
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         145
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         148
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
FT   BINDING         152
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00463"
SQ   SEQUENCE   187 AA;  19090 MW;  93965D368FC63D3F CRC64;
     MIAAAASMSA LGLGLGYLLG AAARKFHVET PPIVEEIAKI LPGTNCGACG FPGCNGLAEA
     MAEGNAPVTA CTPGGRDVAL ALAEIVTVEA GADAGPIAEI EPMVAFVFED HCTGCQKCFK
     RCPTDAIVGG AKQIHTVVMD ACIGCDACIE VCPTEAIVSR VKPKTLKTWY WDKPQPGLVA
     ASAETAA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024