RNFC_RHOCA
ID RNFC_RHOCA Reviewed; 519 AA.
AC Q52716; O08057; Q52712;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Ion-translocating oxidoreductase complex subunit C {ECO:0000255|HAMAP-Rule:MF_00461, ECO:0000305};
DE EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00461, ECO:0000305};
DE AltName: Full=Nitrogen fixation protein RnfC {ECO:0000305};
DE AltName: Full=Rnf electron transport complex subunit C {ECO:0000255|HAMAP-Rule:MF_00461, ECO:0000305};
GN Name=rnfC {ECO:0000255|HAMAP-Rule:MF_00461, ECO:0000303|PubMed:8264535};
OS Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=1061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GENE NAME.
RC STRAIN=B10S;
RX PubMed=8264535; DOI=10.1007/bf00279903;
RA Schmehl M., Jahn A., Meyer zu Vilsendorf A., Hennecke S., Masepohl B.,
RA Schuppler M., Marxer M., Oelze J., Klipp W.;
RT "Identification of a new class of nitrogen fixation genes in Rhodobacter
RT capsulatus: a putative membrane complex involved in electron transport to
RT nitrogenase.";
RL Mol. Gen. Genet. 241:602-615(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, SUBUNIT,
RP SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ATCC 33303 / B10;
RX PubMed=9492268; DOI=10.1046/j.1432-1327.1998.2510054.x;
RA Jouanneau Y., Jeong H.-S., Hugo N., Meyer C., Willison J.C.;
RT "Overexpression in Escherichia coli of the rnf genes from Rhodobacter
RT capsulatus -- characterization of two membrane-bound iron-sulfur
RT proteins.";
RL Eur. J. Biochem. 251:54-64(1998).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane (By
CC similarity). Required for nitrogen fixation. Involved in electron
CC transfer to nitrogenase (PubMed:8264535). {ECO:0000255|HAMAP-
CC Rule:MF_00461, ECO:0000269|PubMed:8264535}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00461};
CC Note=Binds 2 [4Fe-4S] clusters per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00461};
CC -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00461,
CC ECO:0000305|PubMed:9492268}.
CC -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane
CC {ECO:0000255|HAMAP-Rule:MF_00461, ECO:0000269|PubMed:9492268};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00461,
CC ECO:0000269|PubMed:9492268}.
CC -!- INDUCTION: Expression is reduced under iron-limiting conditions.
CC {ECO:0000269|PubMed:9492268}.
CC -!- SIMILARITY: Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00461}.
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DR EMBL; X72888; CAA51399.1; -; Genomic_DNA.
DR EMBL; Y11913; CAA72670.1; -; Genomic_DNA.
DR PIR; S39893; S39893.
DR RefSeq; WP_013068985.1; NZ_VIBE01000016.1.
DR AlphaFoldDB; Q52716; -.
DR SMR; Q52716; -.
DR TCDB; 3.D.6.1.1; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR GeneID; 31492069; -.
DR OMA; YPMGSEK; -.
DR GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.11540; -; 1.
DR HAMAP; MF_00461; RsxC_RnfC; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR010208; Ion_transpt_RnfC/RsxC.
DR InterPro; IPR011538; Nuo51_FMN-bd.
DR InterPro; IPR037225; Nuo51_FMN-bd_sf.
DR InterPro; IPR026902; RnfC_N.
DR InterPro; IPR019554; Soluble_ligand-bd.
DR PANTHER; PTHR43034; PTHR43034; 1.
DR Pfam; PF01512; Complex1_51K; 1.
DR Pfam; PF13237; Fer4_10; 1.
DR Pfam; PF13375; RnfC_N; 1.
DR Pfam; PF10531; SLBB; 1.
DR SUPFAM; SSF142019; SSF142019; 1.
DR TIGRFAMs; TIGR01945; rnfC; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 2.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur;
KW Membrane; Metal-binding; Nitrogen fixation; Repeat; Translocase; Transport.
FT CHAIN 1..519
FT /note="Ion-translocating oxidoreductase complex subunit C"
FT /id="PRO_0000073211"
FT DOMAIN 372..401
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT DOMAIN 411..440
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT REGION 494..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 381
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 384
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 387
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 391
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 420
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 423
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 426
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
FT BINDING 430
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00461"
SQ SEQUENCE 519 AA; 55588 MW; E5451EB2A3FA6026 CRC64;
MRLPSIATLF HPLQSFSIRG GIHPETHKHL TSECEIETMP MPALIRLPLQ QHIGAEAEPI
VKRDDLVLKG QLIAKARGPL SANIHAPTSG RVIAVGHFVA PHASGLPVPT ITIRPDGEDK
WGPHLPRLRP ENAAPEEIAA QVAAAGIVGM GGATFPSAVK LNLRAKYDLT TLIINGAECE
PYLTCDDRLM RERAEEIADG IGIMARALGV KQVFVAIESN KPQAIEAMTR YNRALGYTFK
IHVVPTQYPM GSEKHLVKMI TGQETPARAL TADLGVVVHN IATAHAVHLA VRYGEPLIAR
TVTVSGHGIR RPANLRVLIG TPVSEIIAHC GGFTEEPDRL LLGGPMMGMP IQNPRVPVVK
GTNGILALTA AETPEAKTMP CIRCGRCVQG CPVGLTPFEL NARIHAGDLE GAAKVGLMDC
LACGCCSYNC PANLPLVQSF QFAKGKLSER QSRKHQQEET KRLAAARKAR EEAIAEAKKQ
MMLKRKAEMA AKKKAEEAAA AAAMPPPATA TAIQGEATP
