RNFD_METAC
ID RNFD_METAC Reviewed; 288 AA.
AC Q8TSY3;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Ion-translocating oxidoreductase complex subunit D {ECO:0000255|HAMAP-Rule:MF_00462, ECO:0000305};
DE EC=7.2.1.- {ECO:0000255|HAMAP-Rule:MF_00462, ECO:0000305};
DE AltName: Full=Rnf electron transport complex subunit D {ECO:0000255|HAMAP-Rule:MF_00462, ECO:0000305};
GN Name=rnfD {ECO:0000255|HAMAP-Rule:MF_00462};
GN OrderedLocusNames=MA_0660 {ECO:0000312|EMBL:AAM04102.1};
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
RN [2]
RP FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=23066798; DOI=10.1111/febs.12031;
RA Schlegel K., Welte C., Deppenmeier U., Mueller V.;
RT "Electron transport during aceticlastic methanogenesis by Methanosarcina
RT acetivorans involves a sodium-translocating Rnf complex.";
RL FEBS J. 279:4444-4452(2012).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane. Catalyzes
CC Na(+) transport, most probably coupled to electron transfer from
CC reduced ferredoxin to methanophenazine and heterodisulfide reductase.
CC Involved in heterodisulfide reduction during methanogenesis from
CC acetate. {ECO:0000269|PubMed:23066798}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00462};
CC -!- SUBUNIT: The Rnf complex is probably composed of eight subunits,
CC including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-
CC Rule:MF_00462, ECO:0000305|PubMed:23066798}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00462};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00462}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the rnf operon abolishes growth on
CC acetate and ferredoxin:heterodisulfide oxidoreductase-coupled Na(+)
CC transport. {ECO:0000269|PubMed:23066798}.
CC -!- SIMILARITY: Belongs to the NqrB/RnfD family. {ECO:0000255|HAMAP-
CC Rule:MF_00462}.
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DR EMBL; AE010299; AAM04102.1; -; Genomic_DNA.
DR RefSeq; WP_011020707.1; NC_003552.1.
DR AlphaFoldDB; Q8TSY3; -.
DR SMR; Q8TSY3; -.
DR STRING; 188937.MA_0660; -.
DR TCDB; 3.D.6.1.3; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR EnsemblBacteria; AAM04102; AAM04102; MA_0660.
DR GeneID; 1472552; -.
DR KEGG; mac:MA_0660; -.
DR HOGENOM; CLU_042020_1_0_2; -.
DR InParanoid; Q8TSY3; -.
DR OMA; GWQWINL; -.
DR OrthoDB; 49870at2157; -.
DR PhylomeDB; Q8TSY3; -.
DR BRENDA; 7.2.1.2; 7224.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR HAMAP; MF_00462; RsxD_RnfD; 1.
DR InterPro; IPR004338; NqrB/RnfD.
DR InterPro; IPR011303; RnfD.
DR PANTHER; PTHR30578; PTHR30578; 1.
DR PANTHER; PTHR30578:SF0; PTHR30578:SF0; 1.
DR Pfam; PF03116; NQR2_RnfD_RnfE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Electron transport; Flavoprotein; FMN; Membrane;
KW Phosphoprotein; Reference proteome; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..288
FT /note="Ion-translocating oxidoreductase complex subunit D"
FT /id="PRO_0000443492"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00462"
SQ SEQUENCE 288 AA; 30865 MW; 847737C734B8266D CRC64;
MTSFTVSPPP HIKKKIFIKN LIWSRIVALL PISAAAVYFF GFAALGNIIA SILGAVGIEF
VIQKAFNKKL TIMDGNAIYL GLLLALICPP TLPAWMIFIG GAFAVGVGKH AFGGIGSYTF
HPSLAAWVFL SLAWAQDMLP GTIPILSSFS DLILENGAGF LTDVSPILVL LAGVILILVK
YIEWRIPLSY LLTTVILALV LGDPLAYVVS GTFLLGVFFI ATETVTSPVT QNGRIVYGIL
CGFLTVIYGY FSGNYVWGTL YALLLSNAVA PFIELKTLPK PMGGVANE
