ID   RNFE_AZOVD              Reviewed;         238 AA.
AC   Q9F5Y1; C1DMA3;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Ion-translocating oxidoreductase complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00478};
DE   AltName: Full=Rnf electron transport complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478};
GN   Name=rnfE {ECO:0000255|HAMAP-Rule:MF_00478}; OrderedLocusNames=Avin_50930;
OS   Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=322710;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Rubio L.M., Rangaraj P., Roberts G.P., Ludden P.W.;
RT   "Cloning and mutational analysis of the Azotobacter vinelandii gene
RT   encoding the dinitrogenase gamma subunit.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJ / ATCC BAA-1303;
RX   PubMed=19429624; DOI=10.1128/jb.00504-09;
RA   Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA   Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA   Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A.,
RA   Houmiel K., Imperial J., Kennedy C., Larson T.J., Latreille P., Ligon L.S.,
RA   Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I.,
RA   Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L.,
RA   Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H.,
RA   Dean D.R., Dixon R., Wood D.;
RT   "Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized
RT   to support diverse anaerobic metabolic processes.";
RL   J. Bacteriol. 191:4534-4545(2009).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC       RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00478}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00478}.
CC   -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00478}.
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DR   EMBL; AF302049; AAG29820.1; -; Genomic_DNA.
DR   EMBL; CP001157; ACO81180.1; -; Genomic_DNA.
DR   RefSeq; WP_012703533.1; NC_012560.1.
DR   AlphaFoldDB; Q9F5Y1; -.
DR   SMR; Q9F5Y1; -.
DR   STRING; 322710.Avin_50930; -.
DR   EnsemblBacteria; ACO81180; ACO81180; Avin_50930.
DR   KEGG; avn:Avin_50930; -.
DR   eggNOG; COG4660; Bacteria.
DR   HOGENOM; CLU_046659_1_0_6; -.
DR   OMA; DGFMMGL; -.
DR   OrthoDB; 1782047at2; -.
DR   Proteomes; UP000002424; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00478; RsxE_RnfE; 1.
DR   InterPro; IPR003667; NqrDE/RnfAE.
DR   InterPro; IPR010968; RnfE.
DR   PANTHER; PTHR30586:SF0; PTHR30586:SF0; 1.
DR   Pfam; PF02508; Rnf-Nqr; 1.
DR   PIRSF; PIRSF006102; NQR_DE; 1.
DR   TIGRFAMs; TIGR01948; rnfE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Electron transport; Membrane;
KW   Translocase; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..238
FT                   /note="Ion-translocating oxidoreductase complex subunit E"
FT                   /id="PRO_0000214265"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   CONFLICT        218
FT                   /note="V -> A (in Ref. 1; AAG29820)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   238 AA;  25555 MW;  5701ADD4C9620734 CRC64;
     MSHCGAPSVP EPEKKVPWQY FTSALWQYNV ALVQMLALCP TLAVTTTATN GLGMGLATTL
     VLVMTNALIS SMRHTISPEV RNPVMIGVIA GVVTLTDMAM NAWMHELYKV LGLFIALIVT
     NCAVLGRAES FCLRNPVIPS ILDGAGMGAG FTAVLVVIGG IREILGSGTL FSQASSLLGS
     HFKWMEITVI PDFQGILLAI LPPGAFIVLG FLLAAKRVID RKRAERRQQT HGELVVLQ