ID   RNFE_RHOCA              Reviewed;         243 AA.
AC   P97055; Q52714;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Ion-translocating oxidoreductase complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
DE   AltName: Full=Nitrogen fixation protein RnfE {ECO:0000305};
DE   AltName: Full=Rnf electron transport complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
GN   Name=rnfE {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000303|PubMed:8264535};
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GENE NAME.
RC   STRAIN=B10S;
RX   PubMed=8264535; DOI=10.1007/bf00279903;
RA   Schmehl M., Jahn A., Meyer zu Vilsendorf A., Hennecke S., Masepohl B.,
RA   Schuppler M., Marxer M., Oelze J., Klipp W.;
RT   "Identification of a new class of nitrogen fixation genes in Rhodobacter
RT   capsulatus: a putative membrane complex involved in electron transport to
RT   nitrogenase.";
RL   Mol. Gen. Genet. 241:602-615(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, AND INDUCTION.
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=9492268; DOI=10.1046/j.1432-1327.1998.2510054.x;
RA   Jouanneau Y., Jeong H.-S., Hugo N., Meyer C., Willison J.C.;
RT   "Overexpression in Escherichia coli of the rnf genes from Rhodobacter
RT   capsulatus -- characterization of two membrane-bound iron-sulfur
RT   proteins.";
RL   Eur. J. Biochem. 251:54-64(1998).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane (By
CC       similarity). Required for nitrogen fixation. Involved in electron
CC       transfer to nitrogenase (PubMed:8264535). {ECO:0000255|HAMAP-
CC       Rule:MF_00478, ECO:0000269|PubMed:8264535}.
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC       RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00478,
CC       ECO:0000305|PubMed:9492268}.
CC   -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane
CC       {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- INDUCTION: Expression is reduced under iron-limiting conditions.
CC       {ECO:0000269|PubMed:9492268}.
CC   -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00478}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA51397.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X72888; CAA51397.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Y11913; CAA72666.1; -; Genomic_DNA.
DR   PIR; S39906; S39906.
DR   RefSeq; WP_013068988.1; NZ_VIBE01000016.1.
DR   AlphaFoldDB; P97055; -.
DR   SMR; P97055; -.
DR   TCDB; 3.D.6.1.1; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR   GeneID; 31492072; -.
DR   OMA; DGFMMGL; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00478; RsxE_RnfE; 1.
DR   InterPro; IPR003667; NqrDE/RnfAE.
DR   InterPro; IPR010968; RnfE.
DR   PANTHER; PTHR30586:SF0; PTHR30586:SF0; 1.
DR   Pfam; PF02508; Rnf-Nqr; 1.
DR   PIRSF; PIRSF006102; NQR_DE; 1.
DR   TIGRFAMs; TIGR01948; rnfE; 1.
PE   1: Evidence at protein level;
KW   Electron transport; Membrane; Nitrogen fixation; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..243
FT                   /note="Ion-translocating oxidoreductase complex subunit E"
FT                   /id="PRO_0000214304"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   CONFLICT        193
FT                   /note="G -> A (in Ref. 1; CAA51397)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        205
FT                   /note="R -> P (in Ref. 1; CAA51397)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   243 AA;  25867 MW;  9C3F6EC0F45FEFCF CRC64;
     MSESYAKIAR DGLWDKNIVT GQMLALCPTL AITGTATNGL GMGLATTVVL ILSNVVISAL
     RKTIAPEIRI PAFILIIAAI VTVVDLALNA WLHDLHKVLG LFIALIVTNC AILGRAEAFA
     SRFGVLASAL DGLMMGIGFT LALVVVGAIR EILGSGTLFA QASLLLGPHF AFMELQIFPD
     YPGFLIMILP PGGFLVVGGL FALKRIIDAR KPTIEQEIKQ MRTERVFTAA GVLKPKLETG
     EEA