ID   RNFE_VIBC3              Reviewed;         230 AA.
AC   A5F2S7; C3LZ23;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Ion-translocating oxidoreductase complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
DE   AltName: Full=Rnf electron transport complex subunit E {ECO:0000255|HAMAP-Rule:MF_00478, ECO:0000305};
GN   Name=rnfE {ECO:0000255|HAMAP-Rule:MF_00478};
GN   OrderedLocusNames=VC0395_A0533, VC395_1027;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
RN   [3]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=25831459; DOI=10.1021/acs.biochem.5b00020;
RA   Hreha T.N., Mezic K.G., Herce H.D., Duffy E.B., Bourges A., Pryshchep S.,
RA   Juarez O., Barquera B.;
RT   "Complete topology of the RNF complex from Vibrio cholerae.";
RL   Biochemistry 54:2443-2455(2015).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC       RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00478, ECO:0000269|PubMed:25831459}; Multi-pass membrane
CC       protein {ECO:0000255|HAMAP-Rule:MF_00478}.
CC   -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00478}.
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DR   EMBL; CP000627; ABQ19507.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP09039.1; -; Genomic_DNA.
DR   RefSeq; WP_001293888.1; NZ_JAACZH010000005.1.
DR   AlphaFoldDB; A5F2S7; -.
DR   SMR; A5F2S7; -.
DR   STRING; 345073.VC395_1027; -.
DR   EnsemblBacteria; ABQ19507; ABQ19507; VC0395_A0533.
DR   GeneID; 66939766; -.
DR   KEGG; vco:VC0395_A0533; -.
DR   KEGG; vcr:VC395_1027; -.
DR   PATRIC; fig|345073.21.peg.997; -.
DR   eggNOG; COG4660; Bacteria.
DR   HOGENOM; CLU_046659_1_0_6; -.
DR   OMA; DGFMMGL; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00478; RsxE_RnfE; 1.
DR   InterPro; IPR003667; NqrDE/RnfAE.
DR   InterPro; IPR010968; RnfE.
DR   PANTHER; PTHR30586:SF0; PTHR30586:SF0; 1.
DR   Pfam; PF02508; Rnf-Nqr; 1.
DR   PIRSF; PIRSF006102; NQR_DE; 1.
DR   TIGRFAMs; TIGR01948; rnfE; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Electron transport; Membrane;
KW   Translocase; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..230
FT                   /note="Ion-translocating oxidoreductase complex subunit E"
FT                   /id="PRO_1000072400"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:25831459"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TOPO_DOM        60..68
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:25831459"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TOPO_DOM        90..92
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:25831459"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TOPO_DOM        114..123
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:25831459"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TOPO_DOM        145..181
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:25831459"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00478"
FT   TOPO_DOM        203..230
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:25831459"
SQ   SEQUENCE   230 AA;  24695 MW;  55217DBA3DC29D51 CRC64;
     MSENRTLMLN GMWNNNPALV QLLGLCPLLA VSSTVTNALG LGIATLLVLV GSNVTVSLVR
     DYVPKEVRIP VFVMIIASLV TCVQLLMNAY AYGLYLSLGI FIPLIVTNCI IIGRAEAFAS
     KNDVLPAALD GFWMGLGMTS VLVVLGSLRE IIGNGTLFDG ADLLLGEWAK VLRIEVFHFD
     SAFLLALLPP GAFIGVGFLI AAKSVIDKQI AARQPKQQKQ AIERARVTNV