RNFG_CLOLD
ID RNFG_CLOLD Reviewed; 185 AA.
AC D8GR68;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Proton-translocating ferredoxin:NAD(+) oxidoreductase complex subunit G {ECO:0000305};
DE EC=7.1.1.- {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000269|PubMed:23269825};
DE AltName: Full=Rnf electron transport complex subunit G {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000305};
GN Name=rnfG {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000312|EMBL:ADK14206.1};
GN OrderedLocusNames=CLJU_c11380 {ECO:0000312|EMBL:ADK14206.1};
OS Clostridium ljungdahlii (strain ATCC 55383 / DSM 13528 / PETC).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=748727;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 55383 / DSM 13528 / PETC;
RX PubMed=20616070; DOI=10.1073/pnas.1004716107;
RA Kopke M., Held C., Hujer S., Liesegang H., Wiezer A., Wollherr A.,
RA Ehrenreich A., Liebl W., Gottschalk G., Durre P.;
RT "Clostridium ljungdahlii represents a microbial production platform based
RT on syngas.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:13087-13092(2010).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 55383 / DSM 13528 / PETC;
RX PubMed=23269825; DOI=10.1128/mbio.00406-12;
RA Tremblay P.L., Zhang T., Dar S.A., Leang C., Lovley D.R.;
RT "The Rnf complex of Clostridium ljungdahlii is a proton-translocating
RT ferredoxin:NAD+ oxidoreductase essential for autotrophic growth.";
RL MBio 4:E00406-E00412(2012).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane. Couples
CC electron transfer from reduced ferredoxin to NAD(+) with translocation
CC of H(+) out of the cell. Essential for energy conservation during
CC autotrophic growth. Contributes to ATP synthesis during heterotrophic
CC growth. {ECO:0000269|PubMed:23269825}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00479};
CC -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00479}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00479};
CC Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00479}.
CC -!- SIMILARITY: Belongs to the RnfG family. {ECO:0000255|HAMAP-
CC Rule:MF_00479}.
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DR EMBL; CP001666; ADK14206.1; -; Genomic_DNA.
DR RefSeq; WP_013237803.1; NZ_LITS01000015.1.
DR AlphaFoldDB; D8GR68; -.
DR SMR; D8GR68; -.
DR STRING; 748727.CLJU_c11380; -.
DR EnsemblBacteria; ADK14206; ADK14206; CLJU_c11380.
DR KEGG; clj:CLJU_c11380; -.
DR PATRIC; fig|748727.19.peg.4234; -.
DR eggNOG; COG4659; Bacteria.
DR HOGENOM; CLU_077882_2_1_9; -.
DR OMA; PYIEANQ; -.
DR OrthoDB; 1381933at2; -.
DR BRENDA; 7.1.1.11; 12866.
DR Proteomes; UP000001656; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR HAMAP; MF_00479; RsxG_RnfG; 1.
DR InterPro; IPR007329; FMN-bd.
DR InterPro; IPR010209; Ion_transpt_RnfG/RsxG.
DR PANTHER; PTHR36118; PTHR36118; 1.
DR Pfam; PF04205; FMN_bind; 1.
DR PIRSF; PIRSF006091; E_trnsport_RnfG; 1.
DR SMART; SM00900; FMN_bind; 1.
DR TIGRFAMs; TIGR01947; rnfG; 1.
PE 3: Inferred from homology;
KW Cell membrane; Electron transport; Flavoprotein; FMN; Membrane; NAD;
KW Phosphoprotein; Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..185
FT /note="Proton-translocating ferredoxin:NAD(+)
FT oxidoreductase complex subunit G"
FT /id="PRO_0000443497"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00479"
FT MOD_RES 161
FT /note="FMN phosphoryl threonine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00479"
SQ SEQUENCE 185 AA; 19810 MW; 6E70D7E1C0E04F21 CRC64;
MAKDKDQNSI FAITKNLTIT CFISGIIIAA VYYITSPVAA QKQVQIQNDT MRVLVNDADK
FNKVNGKKDW YAAQKGNKTI AYVVPAESKG YGGAIELLVA VTPDGKVIDF SIVSHNETPG
LGANASKDSF RGQFKDKKAD ALTVVKDKSN TKNIQAMTGA TITSKAVTKG VKEAVEQVTT
FTGGK