RNFG_VIBC3
ID RNFG_VIBC3 Reviewed; 209 AA.
AC A5F2S8; C3LZ24;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Ion-translocating oxidoreductase complex subunit G {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000305};
DE EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000305};
DE AltName: Full=Rnf electron transport complex subunit G {ECO:0000255|HAMAP-Rule:MF_00479, ECO:0000305};
GN Name=rnfG {ECO:0000255|HAMAP-Rule:MF_00479};
GN OrderedLocusNames=VC0395_A0534, VC395_1028;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
RN [3]
RP COFACTOR, PROSTHETIC GROUP AT THR-175, SUBCELLULAR LOCATION, TOPOLOGY, AND
RP MUTAGENESIS OF THR-175.
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=18831535; DOI=10.1021/bi800920j;
RA Backiel J., Juarez O., Zagorevski D.V., Wang Z., Nilges M.J., Barquera B.;
RT "Covalent binding of flavins to RnfG and RnfD in the Rnf complex from
RT Vibrio cholerae.";
RL Biochemistry 47:11273-11284(2008).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=25831459; DOI=10.1021/acs.biochem.5b00020;
RA Hreha T.N., Mezic K.G., Herce H.D., Duffy E.B., Bourges A., Pryshchep S.,
RA Juarez O., Barquera B.;
RT "Complete topology of the RNF complex from Vibrio cholerae.";
RL Biochemistry 54:2443-2455(2015).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane.
CC {ECO:0000255|HAMAP-Rule:MF_00479}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00479,
CC ECO:0000269|PubMed:18831535};
CC -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB, RnfC,
CC RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00479}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00479, ECO:0000269|PubMed:18831535,
CC ECO:0000269|PubMed:25831459}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00479}.
CC -!- SIMILARITY: Belongs to the RnfG family. {ECO:0000255|HAMAP-
CC Rule:MF_00479}.
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DR EMBL; CP000627; ABQ22193.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP09040.1; -; Genomic_DNA.
DR RefSeq; WP_000954366.1; NZ_JAACZH010000005.1.
DR AlphaFoldDB; A5F2S8; -.
DR SMR; A5F2S8; -.
DR STRING; 345073.VC395_1028; -.
DR EnsemblBacteria; ABQ22193; ABQ22193; VC0395_A0534.
DR KEGG; vco:VC0395_A0534; -.
DR KEGG; vcr:VC395_1028; -.
DR PATRIC; fig|345073.21.peg.998; -.
DR eggNOG; COG4659; Bacteria.
DR HOGENOM; CLU_077882_1_0_6; -.
DR OMA; YSGAIHL; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR HAMAP; MF_00479; RsxG_RnfG; 1.
DR InterPro; IPR007329; FMN-bd.
DR InterPro; IPR010209; Ion_transpt_RnfG/RsxG.
DR PANTHER; PTHR36118; PTHR36118; 1.
DR Pfam; PF04205; FMN_bind; 1.
DR PIRSF; PIRSF006091; E_trnsport_RnfG; 1.
DR SMART; SM00900; FMN_bind; 1.
DR TIGRFAMs; TIGR01947; rnfG; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Electron transport; Flavoprotein; FMN;
KW Membrane; Phosphoprotein; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..209
FT /note="Ion-translocating oxidoreductase complex subunit G"
FT /id="PRO_1000072401"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:18831535,
FT ECO:0000305|PubMed:25831459"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00479"
FT TOPO_DOM 30..209
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:18831535,
FT ECO:0000269|PubMed:25831459"
FT MOD_RES 175
FT /note="FMN phosphoryl threonine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00479,
FT ECO:0000269|PubMed:18831535"
FT MUTAGEN 175
FT /note="T->L: Abolishes flavin binding."
FT /evidence="ECO:0000269|PubMed:18831535"
SQ SEQUENCE 209 AA; 22810 MW; F5834674D9823145 CRC64;
MLTAIRKNGL ILAVFACVST GLVALTYALT AEQIQQQEQK QLLQVLNQVI PHKYHDNPLA
QACTLVNDDK LGTAKPMHAY LAQRDGQPTA IAIETIAPDG YNGEIKLIVG IANNGTVLGV
RVLAHQETPG LGDKIDLRIS NWVLGFNGQQ VTADNQDDWK VRKDGGQFDQ FTGATITPRA
VVLAVKKAVE YVNQHQQQLH NQPNPCEGQ
