RNFT2_HUMAN
ID RNFT2_HUMAN Reviewed; 444 AA.
AC Q96EX2; E9PAM7; Q96SU5;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=RING finger and transmembrane domain-containing protein 2;
DE AltName: Full=Transmembrane protein 118;
GN Name=RNFT2; Synonyms=TMEM118;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Teratocarcinoma, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q96EX2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96EX2-2; Sequence=VSP_023465, VSP_023466;
CC Name=3;
CC IsoId=Q96EX2-3; Sequence=VSP_035514, VSP_035515, VSP_035516;
CC Name=4;
CC IsoId=Q96EX2-4; Sequence=VSP_035513;
CC Name=5;
CC IsoId=Q96EX2-5; Sequence=VSP_035515, VSP_035516;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH11878.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK027533; BAB55182.1; -; mRNA.
DR EMBL; AK093164; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC083806; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090013; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471054; EAW98091.1; -; Genomic_DNA.
DR EMBL; BC011878; AAH11878.1; ALT_INIT; mRNA.
DR CCDS; CCDS44987.1; -. [Q96EX2-1]
DR CCDS; CCDS9180.2; -. [Q96EX2-5]
DR RefSeq; NP_001103373.1; NM_001109903.1. [Q96EX2-1]
DR RefSeq; NP_116203.2; NM_032814.3. [Q96EX2-5]
DR AlphaFoldDB; Q96EX2; -.
DR BioGRID; 124340; 3.
DR IntAct; Q96EX2; 1.
DR STRING; 9606.ENSP00000257575; -.
DR iPTMnet; Q96EX2; -.
DR PhosphoSitePlus; Q96EX2; -.
DR BioMuta; RNFT2; -.
DR DMDM; 143216906; -.
DR EPD; Q96EX2; -.
DR jPOST; Q96EX2; -.
DR MassIVE; Q96EX2; -.
DR MaxQB; Q96EX2; -.
DR PaxDb; Q96EX2; -.
DR PeptideAtlas; Q96EX2; -.
DR PRIDE; Q96EX2; -.
DR ProteomicsDB; 19049; -.
DR ProteomicsDB; 76461; -. [Q96EX2-1]
DR ProteomicsDB; 76462; -. [Q96EX2-2]
DR ProteomicsDB; 76463; -. [Q96EX2-3]
DR ProteomicsDB; 76464; -. [Q96EX2-4]
DR Antibodypedia; 31315; 34 antibodies from 16 providers.
DR DNASU; 84900; -.
DR Ensembl; ENST00000257575.9; ENSP00000257575.4; ENSG00000135119.15. [Q96EX2-1]
DR Ensembl; ENST00000392549.7; ENSP00000376332.2; ENSG00000135119.15. [Q96EX2-1]
DR Ensembl; ENST00000407967.7; ENSP00000385669.3; ENSG00000135119.15. [Q96EX2-5]
DR GeneID; 84900; -.
DR KEGG; hsa:84900; -.
DR MANE-Select; ENST00000257575.9; ENSP00000257575.4; NM_001382266.1; NP_001369195.1.
DR UCSC; uc001twb.4; human. [Q96EX2-1]
DR CTD; 84900; -.
DR DisGeNET; 84900; -.
DR GeneCards; RNFT2; -.
DR HGNC; HGNC:25905; RNFT2.
DR HPA; ENSG00000135119; Tissue enhanced (brain, pituitary gland).
DR neXtProt; NX_Q96EX2; -.
DR OpenTargets; ENSG00000135119; -.
DR PharmGKB; PA162401944; -.
DR VEuPathDB; HostDB:ENSG00000135119; -.
DR eggNOG; KOG0802; Eukaryota.
DR eggNOG; KOG4638; Eukaryota.
DR GeneTree; ENSGT00940000158419; -.
DR HOGENOM; CLU_039460_2_0_1; -.
DR InParanoid; Q96EX2; -.
DR OMA; CQADFRE; -.
DR OrthoDB; 1321151at2759; -.
DR PhylomeDB; Q96EX2; -.
DR TreeFam; TF331930; -.
DR PathwayCommons; Q96EX2; -.
DR SignaLink; Q96EX2; -.
DR BioGRID-ORCS; 84900; 6 hits in 1108 CRISPR screens.
DR ChiTaRS; RNFT2; human.
DR GenomeRNAi; 84900; -.
DR Pharos; Q96EX2; Tdark.
DR PRO; PR:Q96EX2; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q96EX2; protein.
DR Bgee; ENSG00000135119; Expressed in buccal mucosa cell and 146 other tissues.
DR ExpressionAtlas; Q96EX2; baseline and differential.
DR Genevisible; Q96EX2; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:1904294; P:positive regulation of ERAD pathway; IEA:InterPro.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR044235; RNFT1/2.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR15860; PTHR15860; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Metal-binding; Reference proteome;
KW Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT CHAIN 1..444
FT /note="RING finger and transmembrane domain-containing
FT protein 2"
FT /id="PRO_0000279508"
FT TOPO_DOM 1..181
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..444
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT ZN_FING 384..422
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 13..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..252
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035513"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035514"
FT VAR_SEQ 238..321
FT /note="QQLYNSLIFLKPNLEMLDFFDLLWIVGIADFVLKYITIALKCLIVALPKIIL
FT AVKSKGKFYLVIEELSQLFRSLVPIQLWYKYI -> EIAPQHSNLGDRVSETLSKKKKK
FT ERREGGRKEGRKERKKAQLWPRCGYPLVSCLYVFLLFLFCHLPHLSLRPVSSSCGHCHP
FT LW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023465"
FT VAR_SEQ 322..444
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023466"
FT VAR_SEQ 401..420
FT /note="HVFCEECLCLWLDRERTCPL -> MLLKGHKKLELEKIDESAGV (in
FT isoform 3 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035515"
FT VAR_SEQ 421..444
FT /note="Missing (in isoform 3 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035516"
SQ SEQUENCE 444 AA; 48965 MW; FC6BF9199C1EBCF8 CRC64;
MWLFTVNQVL RKMQRRHSSN TDNIPPERNR SQALSSEASV DEGGVFESLK AEAASPPALF
SGLSGSLPTS SFPSSLVLGS SAGGGDVFIQ MPASREEGGG RGEGGAYHHR QPHHHFHHGG
HRGGSLLQHV GGDHRGHSEE GGDEQPGTPA PALSELKAVI CWLQKGLPFI LILLAKLCFQ
HKLGIAVCIG MASTFAYANS TLREQVSLKE KRSVLVILWI LAFLAGNTLY VLYTFSSQQL
YNSLIFLKPN LEMLDFFDLL WIVGIADFVL KYITIALKCL IVALPKIILA VKSKGKFYLV
IEELSQLFRS LVPIQLWYKY IMGDDSSNSY FLGGVLIVLY SLCKSFDICG RVGGVRKALK
LLCTSQNYGV RATGQQCTEA GDICAICQAE FREPLILLCQ HVFCEECLCL WLDRERTCPL
CRSVAVDTLR CWKDGATSAH FQVY
