ATPI_SPIOL
ID ATPI_SPIOL Reviewed; 247 AA.
AC P06451;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=ATP synthase subunit a, chloroplastic;
DE AltName: Full=ATP synthase F0 sector subunit a;
DE AltName: Full=F-ATPase subunit IV;
GN Name=atpI;
OS Spinacia oleracea (Spinach).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2443718; DOI=10.1016/0022-2836(87)90690-5;
RA Hudson G.S., Mason J.G., Holton T.A., Koller B., Cox G.B., Whitfeld P.R.,
RA Bottomley W.;
RT "A gene cluster in the spinach and pea chloroplast genomes encoding one CF1
RT and three CF0 subunits of the H+-ATP synthase complex and the ribosomal
RT protein S2.";
RL J. Mol. Biol. 196:283-298(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hennig J., Herrmann R.G.;
RT "Chloroplast ATP synthase of spinach contains nine nonidentical subunit
RT species, six of which are encoded by plastid chromosomes in two operons in
RT a phylogenetically conserved arrangement.";
RL Mol. Gen. Genet. 203:117-128(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Geant d'hiver, and cv. Monatol;
RX PubMed=11292076; DOI=10.1023/a:1006478403810;
RA Schmitz-Linneweber C., Maier R.M., Alcaraz J.-P., Cottet A., Herrmann R.G.,
RA Mache R.;
RT "The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide
RT sequence and gene organization.";
RL Plant Mol. Biol. 45:307-315(2001).
RN [4]
RP PROTEIN SEQUENCE OF 19-34.
RA Fromme P., Graber P., Salnikow J.;
RT "Isolation and identification of a fourth subunit in the membrane part of
RT the chloroplast ATP-synthase.";
RL FEBS Lett. 218:27-30(1987).
CC -!- FUNCTION: Key component of the proton channel; it plays a direct role
CC in the translocation of protons across the membrane.
CC -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC - and CF(0) - the membrane proton channel. CF(1) has five subunits:
CC alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has four main
CC subunits: a, b, b' and c.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Multi-
CC pass membrane protein.
CC -!- SIMILARITY: Belongs to the ATPase A chain family. {ECO:0000305}.
CC -!- CAUTION: This has been proposed to be processed from a precursor
CC (Ref.4); however no signal is predicted by signal sequence detection
CC programs. {ECO:0000305}.
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DR EMBL; X03775; CAA27401.1; -; Genomic_DNA.
DR EMBL; AJ400848; CAB88713.1; -; Genomic_DNA.
DR PIR; S00582; PWSPA6.
DR RefSeq; NP_054920.1; NC_002202.1.
DR PDB; 6FKF; EM; 3.10 A; a=1-247.
DR PDB; 6FKH; EM; 4.20 A; a=1-247.
DR PDB; 6FKI; EM; 4.30 A; a=1-247.
DR PDB; 6VM1; EM; 7.90 A; a=1-247.
DR PDB; 6VM4; EM; 7.08 A; a=1-247.
DR PDB; 6VMB; EM; 5.23 A; a=1-247.
DR PDB; 6VMG; EM; 6.46 A; a=1-247.
DR PDB; 6VOF; EM; 4.51 A; a=1-247.
DR PDB; 6VOH; EM; 4.16 A; a=1-247.
DR PDB; 6VOJ; EM; 4.34 A; a=1-247.
DR PDB; 6VOL; EM; 4.06 A; a=1-247.
DR PDB; 6VON; EM; 3.35 A; a=1-247.
DR PDBsum; 6FKF; -.
DR PDBsum; 6FKH; -.
DR PDBsum; 6FKI; -.
DR PDBsum; 6VM1; -.
DR PDBsum; 6VM4; -.
DR PDBsum; 6VMB; -.
DR PDBsum; 6VMG; -.
DR PDBsum; 6VOF; -.
DR PDBsum; 6VOH; -.
DR PDBsum; 6VOJ; -.
DR PDBsum; 6VOL; -.
DR PDBsum; 6VON; -.
DR AlphaFoldDB; P06451; -.
DR SMR; P06451; -.
DR IntAct; P06451; 1.
DR STRING; 3562.P06451; -.
DR ChEMBL; CHEMBL2366567; -.
DR GeneID; 2715580; -.
DR KEGG; soe:2715580; -.
DR OrthoDB; 1136313at2759; -.
DR PRO; PR:P06451; -.
DR Proteomes; UP000054095; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0045263; C:proton-transporting ATP synthase complex, coupling factor F(o); IEA:UniProtKB-KW.
DR GO; GO:0046933; F:proton-transporting ATP synthase activity, rotational mechanism; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.120.220; -; 1.
DR HAMAP; MF_01393; ATP_synth_a_bact; 1.
DR InterPro; IPR045082; ATP_syn_F0_a_bact/chloroplast.
DR InterPro; IPR000568; ATP_synth_F0_asu.
DR InterPro; IPR023011; ATP_synth_F0_asu_AS.
DR InterPro; IPR035908; F0_ATP_A_sf.
DR PANTHER; PTHR42823; PTHR42823; 1.
DR Pfam; PF00119; ATP-synt_A; 1.
DR PRINTS; PR00123; ATPASEA.
DR SUPFAM; SSF81336; SSF81336; 1.
DR TIGRFAMs; TIGR01131; ATP_synt_6_or_A; 1.
DR PROSITE; PS00449; ATPASE_A; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP synthesis; CF(0); Chloroplast; Direct protein sequencing;
KW Hydrogen ion transport; Ion transport; Membrane; Plastid;
KW Reference proteome; Thylakoid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..247
FT /note="ATP synthase subunit a, chloroplastic"
FT /id="PRO_0000002599"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 37..39
FT /note="GQV -> DKA (in Ref. 2; CAA27401)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="T -> A (in Ref. 2; CAA27401)"
FT /evidence="ECO:0000305"
FT STRAND 28..36
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 37..54
FT /evidence="ECO:0007829|PDB:6FKF"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:6FKF"
FT STRAND 58..60
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 69..84
FT /evidence="ECO:0007829|PDB:6FKF"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 95..114
FT /evidence="ECO:0007829|PDB:6FKF"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 130..132
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 134..154
FT /evidence="ECO:0007829|PDB:6FKF"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 172..207
FT /evidence="ECO:0007829|PDB:6FKF"
FT HELIX 214..243
FT /evidence="ECO:0007829|PDB:6FKF"
SQ SEQUENCE 247 AA; 27087 MW; 013D875F8E409377 CRC64;
MNVLSYSINP LKGLYAISGV EVGQHFYWQI GGFQIHGQVL ITSWVVIAIL LGSAAIAVRS
PQTIPTGGQN FFEYVLEFIR DVSKTQIGEE YRPWVPFIGT MFLFIFVSNW SGALLPWKII
QLPHGELAAP TNDINTTVAL ALLTSVAYFY AGLTKKGLGY FGKYIQPTPI LLPINILEDF
TKPLSLSFRL FGNILADELV VVVLVSLVPL VVPIPVMFLG LFTSGIQALI FATLAAAYIG
ESLEGHH
