ID   RNH2_VIBCH              Reviewed;         206 AA.
AC   P52021; Q9KPW6;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Ribonuclease HII;
DE            Short=RNase HII;
DE            EC=3.1.26.4;
GN   Name=rnhB; OrderedLocusNames=VC_2246;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-206.
RC   STRAIN=El Tor Inaba C6706 / Serotype O1;
RX   PubMed=8917113; DOI=10.1016/0378-1119(96)00155-2;
RA   Franco A.A., Yeh P.E., Johnson J.A., Barry E.M., Guerra H., Maurer R.,
RA   Morris J.G. Jr.;
RT   "Cloning and characterization of dnaE, encoding the catalytic subunit of
RT   replicative DNA polymerase III, from Vibrio cholerae strain C6706.";
RL   Gene 175:281-283(1996).
CC   -!- FUNCTION: Endonuclease that specifically degrades the RNA of RNA-DNA
CC       hybrids. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Manganese or magnesium. Binds 1 divalent metal ion per monomer in
CC       the absence of substrate. May bind a second metal ion after substrate
CC       binding. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RNase HII family. {ECO:0000305}.
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DR   EMBL; AE003852; AAF95390.1; -; Genomic_DNA.
DR   EMBL; U30472; AAC44577.1; -; Genomic_DNA.
DR   PIR; H82100; H82100.
DR   RefSeq; NP_231877.1; NC_002505.1.
DR   RefSeq; WP_001085485.1; NZ_LT906614.1.
DR   AlphaFoldDB; P52021; -.
DR   SMR; P52021; -.
DR   STRING; 243277.VC_2246; -.
DR   PRIDE; P52021; -.
DR   DNASU; 2613168; -.
DR   EnsemblBacteria; AAF95390; AAF95390; VC_2246.
DR   GeneID; 57740869; -.
DR   KEGG; vch:VC_2246; -.
DR   PATRIC; fig|243277.26.peg.2142; -.
DR   eggNOG; COG0164; Bacteria.
DR   HOGENOM; CLU_036532_3_2_6; -.
DR   OMA; YPTKLHL; -.
DR   BioCyc; VCHO:VC2246-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0032299; C:ribonuclease H2 complex; IBA:GO_Central.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IBA:GO_Central.
DR   GO; GO:0043137; P:DNA replication, removal of RNA primer; IBA:GO_Central.
DR   GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR   CDD; cd07182; RNase_HII_bacteria_HII_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00052_B; RNase_HII_B; 1.
DR   InterPro; IPR022898; RNase_HII.
DR   InterPro; IPR001352; RNase_HII/HIII.
DR   InterPro; IPR024567; RNase_HII/HIII_dom.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10954; PTHR10954; 1.
DR   PANTHER; PTHR10954:SF18; PTHR10954:SF18; 1.
DR   Pfam; PF01351; RNase_HII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS51975; RNASE_H_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Manganese; Metal-binding; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..206
FT                   /note="Ribonuclease HII"
FT                   /id="PRO_0000111647"
FT   DOMAIN          19..206
FT                   /note="RNase H type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01319"
FT   BINDING         25
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         26
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        170
FT                   /note="K -> N (in Ref. 2; AAC44577)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   206 AA;  22389 MW;  D37561F0458BF206 CRC64;
     MAKKPSIELP PFEIPAGYAL IAGVDEVGRG PLVGDVVTAA VILDPNRPIM GLNDSKKLSE
     KKRLALFPEI QVKALAWAVG RCSPQEIDEL NIFQATMVAM QRAVAGLRIQ PDLVLIDGNK
     IPKLPMEAQA VVKGDLRVAQ ISAASIIAKV IRDQEMEALD KQYPQFGFAK HKGYPTAAHF
     AAIEQHGVIE QHRKSFGPVK RALGIE