ID   RNHI1_THEGA             Reviewed;          15 AA.
AC   P84784;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 11.
DE   RecName: Full=Ribonuclease;
DE            EC=3.1.-.-;
DE   Flags: Fragment;
OS   Thelephora ganbajun (Ganba fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Thelephorales; Thelephoraceae; Thelephora.
OX   NCBI_TaxID=370292;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND SUBUNIT.
RC   TISSUE=Fruiting body {ECO:0000269|PubMed:15474506};
RX   PubMed=15474506; DOI=10.1016/j.bbrc.2004.09.132;
RA   Wang H.X., Ng T.B.;
RT   "Purification of a novel ribonuclease from dried fruiting bodies of the
RT   edible wild mushroom Thelephora ganbajun.";
RL   Biochem. Biophys. Res. Commun. 324:855-859(2004).
CC   -!- FUNCTION: Has ribonuclease activity towards yeast tRNA and polyuracil,
CC       and decreasing ribonuclease activity towards polyadenine, polycytosine
CC       and polyguanine respectively. Lacks antifungal activity against
CC       M.arachidicola and P.piricola. Inhibits HIV-1 reverse transcriptase.
CC       {ECO:0000269|PubMed:15474506}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 6-7. {ECO:0000269|PubMed:15474506};
CC       Temperature dependence:
CC         Optimum temperature is 45 degrees Celsius. Activity decreases sharply
CC         above 50 degrees Celsius and below 40 degrees Celsius.
CC         {ECO:0000269|PubMed:15474506};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15474506}.
CC   -!- MISCELLANEOUS: Inhibits HIV-1 reverse transcriptase with an IC(50) of
CC       300 nM. {ECO:0000269|PubMed:15474506}.
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DR   GO; GO:0004540; F:ribonuclease activity; IDA:UniProtKB.
DR   GO; GO:0004549; F:tRNA-specific ribonuclease activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Nuclease.
FT   CHAIN           1..>15
FT                   /note="Ribonuclease"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000227528"
FT   NON_TER         15
FT                   /evidence="ECO:0000303|PubMed:15474506"
SQ   SEQUENCE   15 AA;  1581 MW;  D2DF038B2BD32639 CRC64;
     DADIAVWAPP VNAQN