RNHX1_ARATH
ID RNHX1_ARATH Reviewed; 620 AA.
AC P0C2F6; Q9SHY0;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Putative ribonuclease H protein At1g65750;
DE EC=3.1.26.4;
GN OrderedLocusNames=At1g65750; ORFNames=F1E22.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00408};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF23831.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g65760 has been split into 2 genes: At1g65750 and At1g65760.; Evidence={ECO:0000305};
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DR EMBL; AC007234; AAF23831.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A96682; A96682.
DR AlphaFoldDB; P0C2F6; -.
DR SMR; P0C2F6; -.
DR STRING; 3702.AT5G42905.1; -.
DR PRIDE; P0C2F6; -.
DR Araport; AT1G65750; -.
DR eggNOG; KOG1075; Eukaryota.
DR InParanoid; P0C2F6; -.
DR PRO; PR:P0C2F6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0C2F6; baseline and differential.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-EC.
DR CDD; cd06222; RNase_H_like; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR044730; RNase_H-like_dom_plant.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR002156; RNaseH_domain.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR026960; RVT-Znf.
DR Pfam; PF13456; RVT_3; 1.
DR Pfam; PF13966; zf-RVT; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50879; RNASE_H_1; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome.
FT CHAIN 1..620
FT /note="Putative ribonuclease H protein At1g65750"
FT /id="PRO_0000274927"
FT DOMAIN 456..586
FT /note="RNase H type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
FT BINDING 465
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
FT BINDING 505
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
FT BINDING 529
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
FT BINDING 578
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
SQ SEQUENCE 620 AA; 70571 MW; C1889CE19EAC9223 CRC64;
MPVLQKRINK DTFGEILERV SSRMSGWREK TLSFAGRLTL TKAVLSSMPV HSMSTILLPQ
SILNRLDQLS RTFLWGSTAE KKKQHLVKWS KVCSPKKEGG LGVRAAKSMN RALISKVGWR
LLQEKNSLWT LVLQKKYHVG EIRDSRWLIP KGSWSSTWRS IAIGLRDVVS HGVGWIPGDG
QQIRFWTDRW VSGKPLLELD NGERPTDCDT VVAKDLWIPG RGWDFAKIDP YTTNNTRLEL
RAVVLDLVTG ARDRLSWKFS QDGQFSVRSA YEMLTVDEVP RPNMASFFNC LWKVRVPERV
KTFLWLVGNQ AVMTEEERHR RHLSASNVCQ VCKGGVESML HVLRDCPAQL GIWVRVVPQR
RQQGFFSKSL FEWLYDNLGD RSGCEDIPWS TIFAVIIWWG WKWRCGNIFG ENTKCRDRVK
FVKEWAVEVY RAHSGNVLVG ITQPRVERMI GWVSPCVGWV KVNTDGASRG NPGLASAGGV
LRDCTGAWCG GFSLNIGRCS APQAELWGVY YGLYFAWEKK VPRVELEVDS EVIVGFLKTG
ISDSHPLSFL VRLCHGFLQK DWLVRIVHVY REANRLADGL ANYAFSLSLG FHSFDLVPDA
MSSLLREDTL GSTRPRRVRL
