RNK_DROME
ID RNK_DROME Reviewed; 95 AA.
AC Q8MSF5;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Ribonuclease kappa {ECO:0000303|PubMed:26056282, ECO:0000312|FlyBase:FBgn0262116};
DE Short=RNase K;
DE Short=RNase kappa;
DE EC=3.1.-.-;
GN Name=RNASEK {ECO:0000303|PubMed:26056282, ECO:0000312|FlyBase:FBgn0262116};
GN Synonyms=l(2)NC110 {ECO:0000312|FlyBase:FBgn0262116};
GN ORFNames=CG40127 {ECO:0000312|FlyBase:FBgn0262116};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION (MICROBIAL INFECTION).
RX PubMed=26056282; DOI=10.1073/pnas.1424098112;
RA Hackett B.A., Yasunaga A., Panda D., Tartell M.A., Hopkins K.C.,
RA Hensley S.E., Cherry S.;
RT "RNASEK is required for internalization of diverse acid-dependent
RT viruses.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:7797-7802(2015).
CC -!- FUNCTION: Endoribonuclease. {ECO:0000250|UniProtKB:Q7Z0Q2}.
CC -!- FUNCTION: (Microbial infection) Required for the initial stages of
CC clathrin-mediated endocytic uptake of a diverse set of flaviviruses,
CC including dengue and West Nile (PubMed:26056282). Not required for
CC clathrin-mediated endocytosis and macropinocytosis (PubMed:26056282).
CC {ECO:0000269|PubMed:26056282}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RNase K family. {ECO:0000305}.
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DR EMBL; AE013599; EAA46159.1; -; Genomic_DNA.
DR EMBL; AY118852; AAM50712.1; -; mRNA.
DR RefSeq; NP_001036433.1; NM_001042968.2.
DR RefSeq; NP_001260699.1; NM_001273770.2.
DR AlphaFoldDB; Q8MSF5; -.
DR SMR; Q8MSF5; -.
DR BioGRID; 78141; 31.
DR DIP; DIP-18216N; -.
DR IntAct; Q8MSF5; 34.
DR STRING; 7227.FBpp0110588; -.
DR PaxDb; Q8MSF5; -.
DR PRIDE; Q8MSF5; -.
DR DNASU; 3355016; -.
DR EnsemblMetazoa; FBtr0111266; FBpp0110588; FBgn0262116.
DR EnsemblMetazoa; FBtr0334986; FBpp0306999; FBgn0262116.
DR GeneID; 3355016; -.
DR KEGG; dme:Dmel_CG40127; -.
DR UCSC; CG40127-RA; d. melanogaster.
DR CTD; 440400; -.
DR FlyBase; FBgn0262116; RNASEK.
DR VEuPathDB; VectorBase:FBgn0262116; -.
DR eggNOG; ENOG502S351; Eukaryota.
DR GeneTree; ENSGT00940000172351; -.
DR HOGENOM; CLU_140554_2_1_1; -.
DR InParanoid; Q8MSF5; -.
DR OMA; SQNAYNC; -.
DR OrthoDB; 1615928at2759; -.
DR PhylomeDB; Q8MSF5; -.
DR BioGRID-ORCS; 3355016; 1 hit in 1 CRISPR screen.
DR ChiTaRS; RNASEK; fly.
DR GenomeRNAi; 3355016; -.
DR PRO; PR:Q8MSF5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0262116; Expressed in cleaving embryo and 22 other tissues.
DR ExpressionAtlas; Q8MSF5; baseline and differential.
DR Genevisible; Q8MSF5; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004521; F:endoribonuclease activity; ISS:UniProtKB.
DR GO; GO:0098586; P:cellular response to virus; IMP:FlyBase.
DR InterPro; IPR026770; RNase_K.
DR PANTHER; PTHR31733; PTHR31733; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Membrane; Nuclease; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..95
FT /note="Ribonuclease kappa"
FT /id="PRO_0000344228"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 95 AA; 10763 MW; 5FCD0D335D6475D3 CRC64;
MKICGPKLSL CGLIISVWGI VQLVLMGLFF YINSVALIED LPLEEEYHSL EDFYAAANRA
YNQNAYNCWI AACIYVLTLL LSAQQFYMNS RVTAN
