RNN1_NEUCR
ID RNN1_NEUCR Reviewed; 153 AA.
AC P09646; Q1K8C1; Q8WZP7; V5IL75;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Guanyl-specific ribonuclease N1;
DE Short=RNase N1;
DE EC=4.6.1.24;
DE Flags: Precursor;
GN Name=grn; ORFNames=G15G9.140, NCU01045;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [3]
RP PROTEIN SEQUENCE OF 50-153.
RX PubMed=2977130; DOI=10.1093/oxfordjournals.jbchem.a122476;
RA Takahashi K.;
RT "The amino acid sequence of ribonuclease N1, a guanine-specific
RT ribonuclease from the fungus Neurospora crassa.";
RL J. Biochem. 104:375-382(1988).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[RNA] containing guanosine + H2O = an [RNA fragment]-3'-
CC guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA
CC fragment].; EC=4.6.1.24;
CC -!- SIMILARITY: Belongs to the ribonuclease N1/T1 family. {ECO:0000305}.
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DR EMBL; AL670006; CAD21301.1; -; Genomic_DNA.
DR EMBL; CM002240; ESA42488.1; -; Genomic_DNA.
DR PIR; JT0301; NRNCT1.
DR RefSeq; XP_011394457.1; XM_011396155.1.
DR AlphaFoldDB; P09646; -.
DR SMR; P09646; -.
DR EnsemblFungi; ESA42488; ESA42488; NCU01045.
DR GeneID; 3877848; -.
DR KEGG; ncr:NCU01045; -.
DR VEuPathDB; FungiDB:NCU01045; -.
DR HOGENOM; CLU_111658_1_0_1; -.
DR InParanoid; P09646; -.
DR OMA; CGSVCYW; -.
DR BRENDA; 4.6.1.24; 3627.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0046589; F:ribonuclease T1 activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR InterPro; IPR000026; Gua-sp_ribonuclease_N1/T1/U2.
DR InterPro; IPR016191; Ribonuclease/ribotoxin.
DR Pfam; PF00545; Ribonuclease; 1.
DR PIRSF; PIRSF037430; RNase_U2; 1.
DR SUPFAM; SSF53933; SSF53933; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Endonuclease; Hydrolase; Lyase;
KW Nuclease; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..49
FT /evidence="ECO:0000269|PubMed:2977130"
FT /id="PRO_0000030831"
FT CHAIN 50..153
FT /note="Guanyl-specific ribonuclease N1"
FT /id="PRO_0000030832"
FT ACT_SITE 89
FT /evidence="ECO:0000250"
FT ACT_SITE 107
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 141
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT DISULFID 51..59
FT /evidence="ECO:0000269|PubMed:2977130"
FT DISULFID 55..152
FT /evidence="ECO:0000269|PubMed:2977130"
SQ SEQUENCE 153 AA; 16012 MW; 2F00E46B7ED45A39 CRC64;
MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA CMYICGSVCY
SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP TAKPWYEFPI LSSGRVYTGG
SPGADRVIFD SHGNLDMLIT HNGASGNNFV ACN
