ID   AB22B_ARATH             Reviewed;        1229 AA.
AC   Q9LSJ2; F4IZ32;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 2.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=ABC transporter B family member 22;
DE            Short=ABC transporter ABCB.22;
DE            Short=AtABCB22;
DE   AltName: Full=P-glycoprotein 22;
DE   AltName: Full=Putative multidrug resistance protein 21;
GN   Name=ABCB22; Synonyms=MDR21, PGP22; OrderedLocusNames=At3g28415;
GN   ORFNames=MFJ20.11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA   Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT   "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL   J. Biol. Chem. 276:30231-30244(2001).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA   Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA   Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA   Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT   "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL   Trends Plant Sci. 13:151-159(2008).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441};
CC       Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family.
CC       Multidrug resistance exporter (TC 3.A.1.201) subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AEE77442.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAB02858.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB026644; BAB02858.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002686; AEE77442.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; ANM65564.1; -; Genomic_DNA.
DR   RefSeq; NP_001327523.1; NM_001338956.1.
DR   RefSeq; NP_683599.1; NM_148757.1.
DR   AlphaFoldDB; Q9LSJ2; -.
DR   SMR; Q9LSJ2; -.
DR   STRING; 3702.AT3G28415.1; -.
DR   PaxDb; Q9LSJ2; -.
DR   PRIDE; Q9LSJ2; -.
DR   ProteomicsDB; 244497; -.
DR   EnsemblPlants; AT3G28415.2; AT3G28415.2; AT3G28415.
DR   GeneID; 822471; -.
DR   Gramene; AT3G28415.2; AT3G28415.2; AT3G28415.
DR   KEGG; ath:AT3G28415; -.
DR   Araport; AT3G28415; -.
DR   TAIR; locus:504955759; AT3G28415.
DR   eggNOG; KOG0055; Eukaryota.
DR   HOGENOM; CLU_000604_17_2_1; -.
DR   InParanoid; Q9LSJ2; -.
DR   OMA; RWYEMDG; -.
DR   OrthoDB; 186078at2759; -.
DR   PRO; PR:Q9LSJ2; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LSJ2; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1560.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011527; ABC1_TM_dom.
DR   InterPro; IPR036640; ABC1_TM_sf.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR039421; Type_1_exporter.
DR   PANTHER; PTHR24221; PTHR24221; 1.
DR   Pfam; PF00664; ABC_membrane; 2.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF90123; SSF90123; 2.
DR   PROSITE; PS50929; ABC_TM1F; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Glycoprotein; Membrane; Nucleotide-binding;
KW   Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1229
FT                   /note="ABC transporter B family member 22"
FT                   /id="PRO_0000227932"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        145..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        171..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        251..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        661..681
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        703..723
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        782..800
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        807..823
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        885..908
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        923..943
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          22..311
FT                   /note="ABC transmembrane type-1 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          346..582
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          661..949
FT                   /note="ABC transmembrane type-1 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          984..1222
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         381..388
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         1019..1026
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   CARBOHYD        529
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        594
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        758
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1004
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1157
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1229 AA;  134940 MW;  C5052E61E8A39EC3 CRC64;
     MKSFGSVRSI FMHANSVDLV LMGLGLIGAV GDGFITPIIF FITGLLLNDI GDSSFGDKTF
     MHAIMKNAVA LLYVAGASLV ICFVEGYCWT RTGERQASRM REKYLRAVLR QDVGYFDLHV
     TSTSDVITSV SSDTLVIQDV LSEKLPNFLM SASAFVASYI VGFIMLWRLT IVGFPFFILL
     LIPGLMCGRA LINISRKIRE EYNEAGSIAE QAISLVRTVY AFGSERKMIS KFSAALEGSV
     KLGLRQGIAK GIAIGSNGVT YAIWGFMTWY GSRMVMYHGA KGGTIFAVII CITYGGTSLG
     RGLSNLKYFS EAVVAGERII EVIKRVPDID SDNPRGQVLE NIKGEVQFKH VKFMYSSRPE
     TPIFDDLCLR IPSGKSVALV GGSGSGKSTV ISLLQRFYDP IVGEILIDGV SIKKLQVKWL
     RSQMGLVSQE PALFATSIEE NILFGKEDAS FDEVVEAAKS SNAHDFISQF PLGYKTQVGE
     RGVQMSGGQK QRISIARAII KSPTLLLLDE ATSALDSESE RVVQEALDNA TIGRTTIVIA
     HRLSTIRNVD VICVFKNGQI VETGSHEELM ENVDGQYTSL VRLQIMENEE SNDNVSVSMR
     EGQFSNFNKD VKYSSRLSIQ SRSSLFATSS IDTNLAGSIP KDKKPSFKRL MAMNKPEWKH
     ALYGCLSAVL YGALHPIYAY ASGSMVSVYF LTSHDEMKEK TRIYVLLFVG LAVLCFLISI
     IQQYSFAYMG EYLTKRIREN ILSKLLTFEV SWFDEDENSS GSICSRLAKD ANVVRSLVGE
     RVSLLVQTIS AVSVACTLGL AISWKLSIVM IAIQPVVVGC FYTQRIVLKS ISKKAIKAQD
     ESSKLAAEAV SNIRTITAFS SQERILKLLK MVQEGPQREN IRQSWLAGIV LATSRSLMTC
     TSALNYWYGA RLIIDGKITS KAFFELFILF VSTGRVIADA GAMTMDLAKG SDAVGSVFAV
     LDRYTNIEPE KPDGFVPQNI KGQIKFVNVD FAYPTRPDVI IFKNFSIDID EGKSTAIVGP
     SGSGKSTIIG LIERFYDPLK GIVKIDGRDI RSYHLRSLRQ HIGLVSQEPI LFAGTIRENI
     MYGGASDKID ESEIIEAAKA ANAHDFIVTL SDGYDTYCGD RGVQLSGGQK QRIAIARAVL
     KNPSVLLLDE ATSALDNQSE RMVQDALGRL MVGRTSVVIA HRLSTIQNCD TITVLDKGKV
     VECGTHSSLL AKGPTGVYFS LVSLQRTRY