RNPC3_RAT
ID RNPC3_RAT Reviewed; 515 AA.
AC Q4G055;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=RNA-binding region-containing protein 3;
DE AltName: Full=RNA-binding motif protein 40;
DE Short=RNA-binding protein 40;
GN Name=Rnpc3; Synonyms=Rbm40;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 93-515.
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND SER-349, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Participates in pre-mRNA U12-dependent splicing, performed by
CC the minor spliceosome which removes U12-type introns. U12-type introns
CC comprises less than 1% of all non-coding sequences. Binds to the 3'-
CC stem-loop of m(7)G-capped U12 snRNA (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome. Found in a complex with m(7)G-capped U12 snRNA. Interacts
CC with PDCD7 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
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DR EMBL; AABR03021470; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03019434; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03018538; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03019699; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC098742; AAH98742.1; -; mRNA.
DR RefSeq; NP_001094280.1; NM_001100810.1.
DR RefSeq; XP_006233273.1; XM_006233211.3.
DR RefSeq; XP_008759646.1; XM_008761424.2.
DR AlphaFoldDB; Q4G055; -.
DR SMR; Q4G055; -.
DR STRING; 10116.ENSRNOP00000023366; -.
DR iPTMnet; Q4G055; -.
DR PhosphoSitePlus; Q4G055; -.
DR PaxDb; Q4G055; -.
DR PRIDE; Q4G055; -.
DR Ensembl; ENSRNOT00000087714; ENSRNOP00000069232; ENSRNOG00000017310.
DR GeneID; 691538; -.
DR KEGG; rno:691538; -.
DR UCSC; RGD:1582776; rat.
DR CTD; 55599; -.
DR RGD; 1582776; Rnpc3.
DR eggNOG; KOG4206; Eukaryota.
DR GeneTree; ENSGT00530000063786; -.
DR InParanoid; Q4G055; -.
DR OMA; RPINTKK; -.
DR OrthoDB; 1080666at2759; -.
DR PhylomeDB; Q4G055; -.
DR TreeFam; TF324298; -.
DR Reactome; R-RNO-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q4G055; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000017310; Expressed in thymus and 20 other tissues.
DR ExpressionAtlas; Q4G055; baseline and differential.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0005689; C:U12-type spliceosomal complex; ISO:RGD.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IBA:GO_Central.
DR GO; GO:0030626; F:U12 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12238; RRM1_RBM40_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034147; RBM40_RRM1.
DR InterPro; IPR045164; RBM41/RNPC3.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR16105; PTHR16105; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..515
FT /note="RNA-binding region-containing protein 3"
FT /id="PRO_0000311115"
FT DOMAIN 27..102
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 419..502
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 337..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..246
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..351
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 108
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96LT9"
FT MOD_RES 349
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 515 AA; 58037 MW; 28A6CACCCF4CF822 CRC64;
MAVPEPSMPL SRGGPGSASL SPPRGDRTLL VRHLPAELTA EEKEDLLKYF GAQSVRVLSD
KGRLKHTAFA TFPNEKAAIK ALTRLHQLKL LGHTLVVEFA KEQDRVHSSC PASNAEKKKR
LDDTVENDKE KKEPDVLTVE NGIAPNHGLT FPLNSCLKYM YPPPSSTILA NIVNALASVP
KFYVQVLHLM NKMNLPTPFG PITARPPMYE DYVQLHAPLP PTSPQPPEEP PLPDEDEDLS
SKESEYESSD EEDRQRMNKL MELANFQPKR PKTVKPRHVR KKRKIKDMLT VPSPASQSLH
PVLLPSDVFD QPQPVGNKKI EFNISTNVPA ALNKDLETEQ NNEEKNSDSP DTGLDDSNTG
FGKLFPKPNV NITEEIKEDS DEMPSQFISR RELEKGRISR EEMETLSVFR SYEPGEPNCR
IYVKNLARHV QEKDLKFIFG RYVDFSSETQ RIMFDIRLMK EGRMKGQAFV GLPNEKAAAK
ALKEANGYVL FGKPMVVQFA RSARPKHDSK EGKRK
