ID   RNR_AQUAE               Reviewed;         705 AA.
AC   O67834;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Ribonuclease R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            Short=RNase R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            EC=3.1.13.1 {ECO:0000255|HAMAP-Rule:MF_01895};
DE   AltName: Full=VacB protein homolog;
GN   Name=rnr {ECO:0000255|HAMAP-Rule:MF_01895}; Synonyms=vacB;
GN   OrderedLocusNames=aq_2046;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- FUNCTION: 3'-5' exoribonuclease that releases 5'-nucleoside
CC       monophosphates and is involved in maturation of structured RNAs.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01895};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase R subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
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DR   EMBL; AE000657; AAC07792.1; -; Genomic_DNA.
DR   PIR; F70475; F70475.
DR   RefSeq; NP_214403.1; NC_000918.1.
DR   RefSeq; WP_010881339.1; NC_000918.1.
DR   AlphaFoldDB; O67834; -.
DR   SMR; O67834; -.
DR   STRING; 224324.aq_2046; -.
DR   PRIDE; O67834; -.
DR   EnsemblBacteria; AAC07792; AAC07792; aq_2046.
DR   KEGG; aae:aq_2046; -.
DR   PATRIC; fig|224324.8.peg.1580; -.
DR   eggNOG; COG0557; Bacteria.
DR   HOGENOM; CLU_002333_7_0_0; -.
DR   InParanoid; O67834; -.
DR   OMA; DWYEYRS; -.
DR   OrthoDB; 1602988at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 2.
DR   HAMAP; MF_01895; RNase_R; 1.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR040476; CSD2.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR013223; RNase_B_OB_dom.
DR   InterPro; IPR001900; RNase_II/R.
DR   InterPro; IPR022966; RNase_II/R_CS.
DR   InterPro; IPR004476; RNase_II/RNase_R.
DR   InterPro; IPR011805; RNase_R.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF17876; CSD2; 1.
DR   Pfam; PF08206; OB_RNB; 1.
DR   Pfam; PF00773; RNB; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00357; CSP; 1.
DR   SMART; SM00955; RNB; 1.
DR   SMART; SM00316; S1; 2.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF50249; SSF50249; 4.
DR   TIGRFAMs; TIGR00358; 3_prime_RNase; 1.
DR   TIGRFAMs; TIGR02063; RNase_R; 1.
DR   PROSITE; PS01175; RIBONUCLEASE_II; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..705
FT                   /note="Ribonuclease R"
FT                   /id="PRO_0000166395"
FT   DOMAIN          615..696
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01895"
SQ   SEQUENCE   705 AA;  81538 MW;  2C08587229D1B05E CRC64;
     MDKEKLEKEV IKLLSKKKKP LHFLQIAKAL GLGKKERKTL KKVMRKLKKE GKVKVVKGKY
     EYTGEEVVTG TVIAYPGGFG FLEVEGGKDI YIPPFEMVKV FHGDVVKAKV TEFKGKKEVR
     IIKVLKRAKK DIVAKVVFED EQCYVVPLDE NAHHRILLSK KDCQKLKEGE VVVLKITQFP
     TKKSPARGKV IEVLGNPKEK FIAIDVIIRK YNLPTSYPEK VIKEVEAIPE EIPEEEIKRR
     RDLREQLCFT IDPEKAGDFD DAVAIELTPE GYYKLYVHIA DVSYYVREGT ETDKEAYKRG
     FTYYFPDRAL HMLPEKLSAK LCSLRPNEDK LAFTVEMVFD ESGNLKAYDI YESVIRSKAR
     LTYNEALALI VGDPALEKKF PNLVEPLRMM ETLYRILSRK RWEMGSIDFD LPEAEVIVDE
     YGEPTAIYPY ERHVAHRIIE HFMISANETV ALHLEHAGYP CLYRVHEPPD EEKVENLLEI
     LEGLGYKVKR PHEYTPKFFQ KIIEDFEGRP EENLVRFLTL RAMARAKYSP HNVGHFGLAL
     EHYAHFTSPI RRYPDIIVHR LLKKALRGEE IDYEKTLAYL EEAGNHLSKQ ERIADEAEME
     AIDYLKARYM KGRIGEEFIG IITGVVAFGF FVELEENLVE GLVKINTLTD DEYVFDEPAH
     RLVGVRTGKV FRLGDHVKVR CIAVDEERAR VEFELIEKLE KHETL