ID   RNR_BUCAP               Reviewed;         726 AA.
AC   Q8K917;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Ribonuclease R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            Short=RNase R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            EC=3.1.13.1 {ECO:0000255|HAMAP-Rule:MF_01895};
DE   AltName: Full=VacB protein homolog;
GN   Name=rnr {ECO:0000255|HAMAP-Rule:MF_01895}; OrderedLocusNames=BUsg_545;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: 3'-5' exoribonuclease that releases 5'-nucleoside
CC       monophosphates and is involved in maturation of structured RNAs.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01895};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase R subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
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DR   EMBL; AE013218; AAM68084.1; -; Genomic_DNA.
DR   RefSeq; WP_011054050.1; NC_004061.1.
DR   AlphaFoldDB; Q8K917; -.
DR   SMR; Q8K917; -.
DR   STRING; 198804.BUsg_545; -.
DR   EnsemblBacteria; AAM68084; AAM68084; BUsg_545.
DR   KEGG; bas:BUsg_545; -.
DR   eggNOG; COG0557; Bacteria.
DR   HOGENOM; CLU_002333_7_0_6; -.
DR   OMA; DWYEYRS; -.
DR   OrthoDB; 1602988at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 2.
DR   HAMAP; MF_01895; RNase_R; 1.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR040476; CSD2.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR013223; RNase_B_OB_dom.
DR   InterPro; IPR001900; RNase_II/R.
DR   InterPro; IPR022966; RNase_II/R_CS.
DR   InterPro; IPR004476; RNase_II/RNase_R.
DR   InterPro; IPR011805; RNase_R.
DR   InterPro; IPR013668; RNase_R_HTH_12.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   Pfam; PF17876; CSD2; 1.
DR   Pfam; PF08461; HTH_12; 1.
DR   Pfam; PF08206; OB_RNB; 1.
DR   Pfam; PF00773; RNB; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00357; CSP; 1.
DR   SMART; SM00955; RNB; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 4.
DR   TIGRFAMs; TIGR00358; 3_prime_RNase; 1.
DR   TIGRFAMs; TIGR02063; RNase_R; 1.
DR   PROSITE; PS01175; RIBONUCLEASE_II; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; RNA-binding.
FT   CHAIN           1..726
FT                   /note="Ribonuclease R"
FT                   /id="PRO_0000166398"
FT   DOMAIN          642..723
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01895"
SQ   SEQUENCE   726 AA;  84328 MW;  B7C374ECBD7A5416 CRC64;
     MVVDSYQKKE TKKYRNFIPR REQILFLLKT DKDLISQKKL EKKFSINSQE QKKALRRRLR
     AMERDGQIIY TRNRCYITPE NLKIVTGKVI GHRDGYGFLR TETFKDDLWL SIEQMKLCIH
     GDVILAHIVK SDRKGRNSAK VLKILRPNDV LIVGRYCVDN KKKFVIPNDT RFNFKIFILD
     SLISNENISI GTIVVVKLRE NATKKSKIQG TIVEVLGKEM GTNLAIKIAL RTHCIPYLWS
     KEVEYQLCGI KSKINEKDFK NRIDLRHLPF FTIDEEDARD FDDAIFCKKK TNGEKGWKLW
     VAISDVSYYI QPDTALDKAA SKRGTSIYFP SLVIPMLPEK ISIDVCSLNP NAERLSLICE
     MNLSNKGELI TYKHYEAVIC SHGRFTYNEI FKIWNGDIEL CFKYKKLLKY IQNLSSLQKI
     LKKYNVSKRG IYFENIEAKF ILDSNYRIKN ISQNIRNDAH KFIESCMILA NIASAEFVKK
     HKSPVLFRNH DRPDKDSIIN FRSVLKKLGL SLLGGEIPES TDYSELLKKI STRPDYEMIQ
     TILLRSMKQA VYSPDNRGHF GLSLSSYVHF TSPIRRYPDL LVHRVIKNLL LKEKNLSKYH
     LYNLNEVTKI GLHCSMTERR ADEATRDVLD WLKCDFMQKK IGDVLTGVIS NVTSFGFFVR
     LNQFFIDGLV HIATLIDDYY YFDSIGLKLI GKSSKNTYCL GDTLKVKVIS VNLNERKIEL
     SLYMSR