ID   RNR_THEMA               Reviewed;         710 AA.
AC   Q9WZI1;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=Ribonuclease R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            Short=RNase R {ECO:0000255|HAMAP-Rule:MF_01895};
DE            EC=3.1.13.1 {ECO:0000255|HAMAP-Rule:MF_01895};
DE   AltName: Full=VacB protein homolog;
GN   Name=rnr {ECO:0000255|HAMAP-Rule:MF_01895}; Synonyms=vacB;
GN   OrderedLocusNames=TM_0722;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
CC   -!- FUNCTION: 3'-5' exoribonuclease that releases 5'-nucleoside
CC       monophosphates and is involved in maturation of structured RNAs.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.13.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01895};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01895}.
CC   -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase R subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01895}.
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DR   EMBL; AE000512; AAD35804.1; -; Genomic_DNA.
DR   PIR; H72341; H72341.
DR   RefSeq; NP_228531.1; NC_000853.1.
DR   RefSeq; WP_004081010.1; NZ_CP011107.1.
DR   AlphaFoldDB; Q9WZI1; -.
DR   SMR; Q9WZI1; -.
DR   STRING; 243274.THEMA_01050; -.
DR   EnsemblBacteria; AAD35804; AAD35804; TM_0722.
DR   KEGG; tma:TM0722; -.
DR   eggNOG; COG0557; Bacteria.
DR   InParanoid; Q9WZI1; -.
DR   OMA; DWYEYRS; -.
DR   OrthoDB; 1602988at2; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 3.
DR   HAMAP; MF_01895; RNase_R; 1.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR040476; CSD2.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR013223; RNase_B_OB_dom.
DR   InterPro; IPR001900; RNase_II/R.
DR   InterPro; IPR022966; RNase_II/R_CS.
DR   InterPro; IPR004476; RNase_II/RNase_R.
DR   InterPro; IPR011805; RNase_R.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   Pfam; PF17876; CSD2; 1.
DR   Pfam; PF08206; OB_RNB; 1.
DR   Pfam; PF00773; RNB; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00357; CSP; 1.
DR   SMART; SM00955; RNB; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 4.
DR   TIGRFAMs; TIGR00358; 3_prime_RNase; 1.
DR   TIGRFAMs; TIGR02063; RNase_R; 1.
DR   PROSITE; PS01175; RIBONUCLEASE_II; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..710
FT                   /note="Ribonuclease R"
FT                   /id="PRO_0000166412"
FT   DOMAIN          625..705
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01895"
SQ   SEQUENCE   710 AA;  81783 MW;  5EC09EBAE3B81912 CRC64;
     MSSIRKKDVE RFILSDDYKP MVLKELYKKF RAKTREQRKK VREVVKKLEK EGRIFRDSRG
     RYRKLGEDLK VGTIEFTRSG YVAFVITDDF EEIAVPVEDT KYAMHKDRVV VEITGTWRGL
     PRGRVVRVLE RGLKRVVGVF DHKGTFGFVV PDDPKIAYDF YVAPENIDGA KPNQKVIAEI
     LKYPSPGKNP EAKVVKVLGD LDDPSIDLPS VIVKHDLPEP GEFPEEVIRE ANAIPARVRK
     KDLVGRKDLR DKVIVTIDGE DAKDFDDAIS VEKLPNGNYL LGVHIADVSH YVKEGSALDQ
     EAFKRGTSVY LIDTVIPMLP FRLSNGICSL VEGKDRLTMS VEMEIDRDGR VVRYDVYPSV
     IKSKKRMIYE RVNEFLEDPS SMKEYEPFKD LIYNAVELAE ILREARRKRG AILDIESDEV
     KVILDENGQV VDIVPRKRGI AEKLIEEFMI RANETVAEIF DHAGLPFMYR VHEEPDPETI
     FQLKNYLEAM GIRAKFSHKI HPGMLQKLLE KVKDHPLRSS VERLLVRSMK RAMYSAVNIG
     HFGLASYAYT HFTSPIRRYP DLVVHRLLKL YLEQNGYFTP EQIEKFSKVL PKIAKHCSRR
     ERVADEAEWD LVAMKKVEYI ARHIGEVFNV VVTNITKFGL FVEIPEKSIS GLVHISTLDD
     YYYYDETRNM LIGKRKGKVF RLGDVLKAKV VRADKIRGEI DFVLVEEDEE