ID   RNS_ECOLX               Reviewed;         265 AA.
AC   P16114;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Regulatory protein Rns;
GN   Name=rns;
OS   Escherichia coli.
OG   Plasmid pDEP23.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2563591; DOI=10.1073/pnas.86.3.963;
RA   Caron J., Coffield L.M., Scott J.R.;
RT   "A plasmid-encoded regulatory gene, rns, required for expression of the CS1
RT   and CS2 adhesins of enterotoxigenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:963-967(1989).
CC   -!- FUNCTION: Required for the expression of the CS1 and CS2 adhesins of
CC       enterotoxygenic E.coli.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR   EMBL; J04166; AAA24419.1; -; Genomic_DNA.
DR   PIR; A31457; A31457.
DR   RefSeq; WP_000346363.1; NZ_NNTT01000132.1.
DR   PDB; 6XIU; X-ray; 3.00 A; A/B=1-265.
DR   PDB; 6XIV; X-ray; 2.80 A; A/B=1-265.
DR   PDBsum; 6XIU; -.
DR   PDBsum; 6XIV; -.
DR   AlphaFoldDB; P16114; -.
DR   SMR; P16114; -.
DR   PATRIC; fig|562.7075.peg.4897; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..265
FT                   /note="Regulatory protein Rns"
FT                   /id="PRO_0000194576"
FT   DOMAIN          164..261
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        181..202
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        228..251
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   HELIX           6..8
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          11..18
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          23..26
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          28..38
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          41..43
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          48..51
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          56..65
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           78..92
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           105..107
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   STRAND          109..112
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           116..123
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           130..142
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   TURN            145..147
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           148..156
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           162..170
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   TURN            172..175
FT                   /evidence="ECO:0007829|PDB:6XIU"
FT   HELIX           180..186
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           191..199
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   TURN            200..202
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           205..223
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           228..235
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           240..251
FT                   /evidence="ECO:0007829|PDB:6XIV"
FT   HELIX           255..262
FT                   /evidence="ECO:0007829|PDB:6XIV"
SQ   SEQUENCE   265 AA;  30831 MW;  0CCFB8A818F6E0CE CRC64;
     MDFKYTEEKE TIKINNIMIH KYTVLYTSNC IMDIYSEEEK ITCFSNRLVF LERGVNISVR
     MQKQILSEKP YVAFRLNGDM LRHLKDALMI IYGMSKIDTN ACRSMSRKIM TTEVNKTLLD
     ELKNINSHDN SAFISSLIYL ISKLENNEKI IESIYISSVS FFSDKVRNLI EKDLSRKWTL
     GIIADAFNAS EITIRKRLES ENTNFNQILM QLRMSKAALL LLENSYQISQ ISNMIGISSA
     SYFIRIFNKH YGVTPKQFFT YFKGG